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Transcriptome profiling shows gene regulation patterns in ginsenoside pathway in response to methyl jasmonate in Panax Quinquefolium adventitious root

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ABSTRACT

Here, we combine elicitors and transcriptomics to investigate the inducible biosynthesis of the ginsenoside from the Panax quinquefolium. Treatment of P. quinquefolium adventitious root with methyl jasmonate (MJ) results in an increase in ginsenoside content (43.66 mg/g compared to 8.32 mg/g in control group). Therefore, we sequenced the transcriptome of native and MJ treated adventitious root in order to elucidate the key differentially expressed genes (DEGs) in the ginsenoside biosynthetic pathway. Through DEG analysis, we found that 5,759 unigenes were up-regulated and 6,389 unigenes down-regulated in response to MJ treatment. Several defense-related genes (48) were identified, participating in salicylic acid (SA), jasmonic acid (JA), nitric oxide (NO) and abscisic acid (ABA) signal pathway. Additionally, we mapped 72 unigenes to the ginsenoside biosynthetic pathway. Four cytochrome P450s (CYP450) were likely to catalyze hydroxylation at C-16 (c15743_g1, c39772_g1, c55422_g1) and C-30 (c52011_g1) of the triterpene backbone. UDP-xylose synthases (c52571_g3) was selected as the candidate, which was likely to involve in ginsenoside Rb3 biosynthesis.

No MeSH data available.


Functional classification and pathway assignment of unigenes by KEGG.
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f4: Functional classification and pathway assignment of unigenes by KEGG.

Mentions: Pathway-based analysis provides information on biological functions and the synthesis of secondary metabolites, particularly at the molecular level. For MJ treated P. quinquefolium adventitious roots, a total of 16,371 transcripts were assigned to 32 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways (Fig. 4). As shown in Fig. 4, the majority of KEGG assigned transcripts were involved in signal transduction (1,050). A large pool of transcripts fell within the area of carbohydrate metabolism biosynthesis (898). Additional transcripts were mapped to the area of lipid metabolism biosynthesis (467) and metabolism of terpenoids and polyketides biosynthesis (258).


Transcriptome profiling shows gene regulation patterns in ginsenoside pathway in response to methyl jasmonate in Panax Quinquefolium adventitious root
Functional classification and pathway assignment of unigenes by KEGG.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120341&req=5

f4: Functional classification and pathway assignment of unigenes by KEGG.
Mentions: Pathway-based analysis provides information on biological functions and the synthesis of secondary metabolites, particularly at the molecular level. For MJ treated P. quinquefolium adventitious roots, a total of 16,371 transcripts were assigned to 32 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways (Fig. 4). As shown in Fig. 4, the majority of KEGG assigned transcripts were involved in signal transduction (1,050). A large pool of transcripts fell within the area of carbohydrate metabolism biosynthesis (898). Additional transcripts were mapped to the area of lipid metabolism biosynthesis (467) and metabolism of terpenoids and polyketides biosynthesis (258).

View Article: PubMed Central - PubMed

ABSTRACT

Here, we combine elicitors and transcriptomics to investigate the inducible biosynthesis of the ginsenoside from the Panax quinquefolium. Treatment of P. quinquefolium adventitious root with methyl jasmonate (MJ) results in an increase in ginsenoside content (43.66 mg/g compared to 8.32 mg/g in control group). Therefore, we sequenced the transcriptome of native and MJ treated adventitious root in order to elucidate the key differentially expressed genes (DEGs) in the ginsenoside biosynthetic pathway. Through DEG analysis, we found that 5,759 unigenes were up-regulated and 6,389 unigenes down-regulated in response to MJ treatment. Several defense-related genes (48) were identified, participating in salicylic acid (SA), jasmonic acid (JA), nitric oxide (NO) and abscisic acid (ABA) signal pathway. Additionally, we mapped 72 unigenes to the ginsenoside biosynthetic pathway. Four cytochrome P450s (CYP450) were likely to catalyze hydroxylation at C-16 (c15743_g1, c39772_g1, c55422_g1) and C-30 (c52011_g1) of the triterpene backbone. UDP-xylose synthases (c52571_g3) was selected as the candidate, which was likely to involve in ginsenoside Rb3 biosynthesis.

No MeSH data available.