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Transcriptome profiling shows gene regulation patterns in ginsenoside pathway in response to methyl jasmonate in Panax Quinquefolium adventitious root

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ABSTRACT

Here, we combine elicitors and transcriptomics to investigate the inducible biosynthesis of the ginsenoside from the Panax quinquefolium. Treatment of P. quinquefolium adventitious root with methyl jasmonate (MJ) results in an increase in ginsenoside content (43.66 mg/g compared to 8.32 mg/g in control group). Therefore, we sequenced the transcriptome of native and MJ treated adventitious root in order to elucidate the key differentially expressed genes (DEGs) in the ginsenoside biosynthetic pathway. Through DEG analysis, we found that 5,759 unigenes were up-regulated and 6,389 unigenes down-regulated in response to MJ treatment. Several defense-related genes (48) were identified, participating in salicylic acid (SA), jasmonic acid (JA), nitric oxide (NO) and abscisic acid (ABA) signal pathway. Additionally, we mapped 72 unigenes to the ginsenoside biosynthetic pathway. Four cytochrome P450s (CYP450) were likely to catalyze hydroxylation at C-16 (c15743_g1, c39772_g1, c55422_g1) and C-30 (c52011_g1) of the triterpene backbone. UDP-xylose synthases (c52571_g3) was selected as the candidate, which was likely to involve in ginsenoside Rb3 biosynthesis.

No MeSH data available.


Histogram of unigene KOG classification.
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f3: Histogram of unigene KOG classification.

Mentions: COG is a classification system based on orthologous genes. Orthologous genes have the same function and a common ancestor. We annotated 12,354 unigenes to 26 groups using the COG database (Fig. 3). The largest number (1,929) of the annotated unigenes fell within the general functional prediction only (R), while the fewest number of unigenes (1) were annotated as an unnamed protein (X). Additional assignments included 593 unigenes within the secondary metabolites biosynthesis, transport and catabolism (Q) category and 79 as defense mechanism unigenes (V) (Fig. 3).


Transcriptome profiling shows gene regulation patterns in ginsenoside pathway in response to methyl jasmonate in Panax Quinquefolium adventitious root
Histogram of unigene KOG classification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120341&req=5

f3: Histogram of unigene KOG classification.
Mentions: COG is a classification system based on orthologous genes. Orthologous genes have the same function and a common ancestor. We annotated 12,354 unigenes to 26 groups using the COG database (Fig. 3). The largest number (1,929) of the annotated unigenes fell within the general functional prediction only (R), while the fewest number of unigenes (1) were annotated as an unnamed protein (X). Additional assignments included 593 unigenes within the secondary metabolites biosynthesis, transport and catabolism (Q) category and 79 as defense mechanism unigenes (V) (Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

Here, we combine elicitors and transcriptomics to investigate the inducible biosynthesis of the ginsenoside from the Panax quinquefolium. Treatment of P. quinquefolium adventitious root with methyl jasmonate (MJ) results in an increase in ginsenoside content (43.66 mg/g compared to 8.32 mg/g in control group). Therefore, we sequenced the transcriptome of native and MJ treated adventitious root in order to elucidate the key differentially expressed genes (DEGs) in the ginsenoside biosynthetic pathway. Through DEG analysis, we found that 5,759 unigenes were up-regulated and 6,389 unigenes down-regulated in response to MJ treatment. Several defense-related genes (48) were identified, participating in salicylic acid (SA), jasmonic acid (JA), nitric oxide (NO) and abscisic acid (ABA) signal pathway. Additionally, we mapped 72 unigenes to the ginsenoside biosynthetic pathway. Four cytochrome P450s (CYP450) were likely to catalyze hydroxylation at C-16 (c15743_g1, c39772_g1, c55422_g1) and C-30 (c52011_g1) of the triterpene backbone. UDP-xylose synthases (c52571_g3) was selected as the candidate, which was likely to involve in ginsenoside Rb3 biosynthesis.

No MeSH data available.