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Transcriptome profiling shows gene regulation patterns in ginsenoside pathway in response to methyl jasmonate in Panax Quinquefolium adventitious root

View Article: PubMed Central - PubMed

ABSTRACT

Here, we combine elicitors and transcriptomics to investigate the inducible biosynthesis of the ginsenoside from the Panax quinquefolium. Treatment of P. quinquefolium adventitious root with methyl jasmonate (MJ) results in an increase in ginsenoside content (43.66 mg/g compared to 8.32 mg/g in control group). Therefore, we sequenced the transcriptome of native and MJ treated adventitious root in order to elucidate the key differentially expressed genes (DEGs) in the ginsenoside biosynthetic pathway. Through DEG analysis, we found that 5,759 unigenes were up-regulated and 6,389 unigenes down-regulated in response to MJ treatment. Several defense-related genes (48) were identified, participating in salicylic acid (SA), jasmonic acid (JA), nitric oxide (NO) and abscisic acid (ABA) signal pathway. Additionally, we mapped 72 unigenes to the ginsenoside biosynthetic pathway. Four cytochrome P450s (CYP450) were likely to catalyze hydroxylation at C-16 (c15743_g1, c39772_g1, c55422_g1) and C-30 (c52011_g1) of the triterpene backbone. UDP-xylose synthases (c52571_g3) was selected as the candidate, which was likely to involve in ginsenoside Rb3 biosynthesis.

No MeSH data available.


Accumulation of signal molecules:NO (a), SA (b), JA (c), ABA (d) in adventitious roots of P. quinquefolium that were affected by MJ.
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f1: Accumulation of signal molecules:NO (a), SA (b), JA (c), ABA (d) in adventitious roots of P. quinquefolium that were affected by MJ.

Mentions: The results of this study showed that MJ can induce NO, SA, JA and ABA accumulation in P. quinquefolium adventitious roots. As shown in Fig. 1, increase of NO, SA, JA and ABA were observed, reaching the highest level (732.44 μmol·gprotein−1, 0.08 ng·g−1, 1.08 ng·g−1 and 21.11 ng·mL−1) at 24 h, respectively.


Transcriptome profiling shows gene regulation patterns in ginsenoside pathway in response to methyl jasmonate in Panax Quinquefolium adventitious root
Accumulation of signal molecules:NO (a), SA (b), JA (c), ABA (d) in adventitious roots of P. quinquefolium that were affected by MJ.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120341&req=5

f1: Accumulation of signal molecules:NO (a), SA (b), JA (c), ABA (d) in adventitious roots of P. quinquefolium that were affected by MJ.
Mentions: The results of this study showed that MJ can induce NO, SA, JA and ABA accumulation in P. quinquefolium adventitious roots. As shown in Fig. 1, increase of NO, SA, JA and ABA were observed, reaching the highest level (732.44 μmol·gprotein−1, 0.08 ng·g−1, 1.08 ng·g−1 and 21.11 ng·mL−1) at 24 h, respectively.

View Article: PubMed Central - PubMed

ABSTRACT

Here, we combine elicitors and transcriptomics to investigate the inducible biosynthesis of the ginsenoside from the Panax quinquefolium. Treatment of P. quinquefolium adventitious root with methyl jasmonate (MJ) results in an increase in ginsenoside content (43.66 mg/g compared to 8.32 mg/g in control group). Therefore, we sequenced the transcriptome of native and MJ treated adventitious root in order to elucidate the key differentially expressed genes (DEGs) in the ginsenoside biosynthetic pathway. Through DEG analysis, we found that 5,759 unigenes were up-regulated and 6,389 unigenes down-regulated in response to MJ treatment. Several defense-related genes (48) were identified, participating in salicylic acid (SA), jasmonic acid (JA), nitric oxide (NO) and abscisic acid (ABA) signal pathway. Additionally, we mapped 72 unigenes to the ginsenoside biosynthetic pathway. Four cytochrome P450s (CYP450) were likely to catalyze hydroxylation at C-16 (c15743_g1, c39772_g1, c55422_g1) and C-30 (c52011_g1) of the triterpene backbone. UDP-xylose synthases (c52571_g3) was selected as the candidate, which was likely to involve in ginsenoside Rb3 biosynthesis.

No MeSH data available.