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The Effect of Silica Nanoparticles on Human Corneal Epithelial Cells

View Article: PubMed Central - PubMed

ABSTRACT

Ocular drug delivery is an interesting field in current research. Silica nanoparticles (SiNPs) are promising drug carriers for ophthalmic drug delivery. However, little is known about the toxicity of SiNPs on ocular surface cells such as human corneal epithelial cells (HCECs). In this study, we evaluated the cytotoxicity induced by 50, 100 and 150 nm sizes of SiNPs on cultured HCECs for up to 48 hours. SiNPs were up-taken by HCECs inside cytoplasmic vacuoles. Cellular reactive oxygen species generation was mildly elevated, dose dependently, with SiNPs, but no significant decrease of cellular viability was observed up to concentrations of 100 μg/ml for three different sized SiNPs. Western blot assays revealed that both cellular autophagy and mammalian target of rapamycin (mTOR) pathways were activated with the addition of SiNPs. Our findings suggested that 50, 100 and 150 nm sized SiNPs did not induce significant cytotoxicity in cultured HCECs.

No MeSH data available.


Related in: MedlinePlus

Cellular viability assay.Cellular viability using CCK-8 (A) and lactose dehydrogenase (LDH) after 24 h exposure to 25 μg/mL, 50 μg/mL, or 100 μg/mL of SiNP treated HCEC. No significant changes were observed with SiNPs. Quadruplicates of each treatment group were used in each independent experiment. Values are the mean ± SEM from four independent experiments.
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f6: Cellular viability assay.Cellular viability using CCK-8 (A) and lactose dehydrogenase (LDH) after 24 h exposure to 25 μg/mL, 50 μg/mL, or 100 μg/mL of SiNP treated HCEC. No significant changes were observed with SiNPs. Quadruplicates of each treatment group were used in each independent experiment. Values are the mean ± SEM from four independent experiments.

Mentions: HCEC viability was not affected with the treatment of SiNPs (Fig. 6A) Accordingly, LDH level was unchanged with the treatment of SiNPs (Fig. 6B). The size and concentration of SiNPs did not affect the viability or the LDH levels significantly. In addition, TUNEL assay following 24 h treatment of SiNPs showed that none of the SiNPs sizes induced apoptosis (Fig. 7). Only 6.46, 8.55, and 7.89% of apoptotic cells were detected with the treatment of 100 μg/mL of 50, 100, 150 nm SiNPs, respectively. When considering 3.83~7.49% of apoptotic cells in the negative controls, the proportion of apoptotic cells with SiNPs treatment were considered to be in the normal range.


The Effect of Silica Nanoparticles on Human Corneal Epithelial Cells
Cellular viability assay.Cellular viability using CCK-8 (A) and lactose dehydrogenase (LDH) after 24 h exposure to 25 μg/mL, 50 μg/mL, or 100 μg/mL of SiNP treated HCEC. No significant changes were observed with SiNPs. Quadruplicates of each treatment group were used in each independent experiment. Values are the mean ± SEM from four independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120337&req=5

f6: Cellular viability assay.Cellular viability using CCK-8 (A) and lactose dehydrogenase (LDH) after 24 h exposure to 25 μg/mL, 50 μg/mL, or 100 μg/mL of SiNP treated HCEC. No significant changes were observed with SiNPs. Quadruplicates of each treatment group were used in each independent experiment. Values are the mean ± SEM from four independent experiments.
Mentions: HCEC viability was not affected with the treatment of SiNPs (Fig. 6A) Accordingly, LDH level was unchanged with the treatment of SiNPs (Fig. 6B). The size and concentration of SiNPs did not affect the viability or the LDH levels significantly. In addition, TUNEL assay following 24 h treatment of SiNPs showed that none of the SiNPs sizes induced apoptosis (Fig. 7). Only 6.46, 8.55, and 7.89% of apoptotic cells were detected with the treatment of 100 μg/mL of 50, 100, 150 nm SiNPs, respectively. When considering 3.83~7.49% of apoptotic cells in the negative controls, the proportion of apoptotic cells with SiNPs treatment were considered to be in the normal range.

View Article: PubMed Central - PubMed

ABSTRACT

Ocular drug delivery is an interesting field in current research. Silica nanoparticles (SiNPs) are promising drug carriers for ophthalmic drug delivery. However, little is known about the toxicity of SiNPs on ocular surface cells such as human corneal epithelial cells (HCECs). In this study, we evaluated the cytotoxicity induced by 50, 100 and 150 nm sizes of SiNPs on cultured HCECs for up to 48 hours. SiNPs were up-taken by HCECs inside cytoplasmic vacuoles. Cellular reactive oxygen species generation was mildly elevated, dose dependently, with SiNPs, but no significant decrease of cellular viability was observed up to concentrations of 100 μg/ml for three different sized SiNPs. Western blot assays revealed that both cellular autophagy and mammalian target of rapamycin (mTOR) pathways were activated with the addition of SiNPs. Our findings suggested that 50, 100 and 150 nm sized SiNPs did not induce significant cytotoxicity in cultured HCECs.

No MeSH data available.


Related in: MedlinePlus