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Fabrication of functional hollow microspheres constructed from MOF shells: Promising drug delivery systems with high loading capacity and targeted transport

View Article: PubMed Central - PubMed

ABSTRACT

An advanced multifunctional, hollow metal-organic framework (MOF) drug delivery system with a high drug loading level and targeted delivery was designed and fabricated for the first time and applied to inhibit tumour cell growth. This hollow MOF targeting drug delivery system was prepared via a simple post-synthetic surface modification procedure, starting from hollow ZIF-8 successfully obtained for the first time via a mild phase transformation under solvothermal conditions. As a result, the hollow ZIF-8 exhibits a higher loading capacity for the model anticancer drug 5-fluorouracil (5-FU). Subsequently, 5-FU-loaded ZIF-8 was encapsulated into polymer layers (FA-CHI-5-FAM) with three components: a chitosan (CHI) backbone, the imaging agent 5-carboxyfluorescein (5-FAM), and the targeting reagent folic acid (FA). Thus, an advanced drug delivery system, ZIF-8/5-FU@FA-CHI-5-FAM, was fabricated. A cell imaging assay demonstrated that ZIF-8/5-FU@FA-CHI-5-FAM could target and be taken up by MGC-803 cells. Furthermore, the as-prepared ZIF-8/5-FU@FA-CHI-5-FAM exhibited stronger cell growth inhibitory effects on MGC-803 cells because of the release of 5-FU, as confirmed by a cell viability assay. In addition, a drug release experiment in vitro indicated that ZIF-8/5-FU@FA-CHI-5-FAM exhibited high loading capacity (51%) and a sustained drug release behaviour. Therefore, ZIF-8/5-FU@FA-CHI-5-FAM could provide targeted drug transportation, imaging tracking and localized sustained release.

No MeSH data available.


Drug release profiles for ZIF-8/5-FU@FA-CHI-5-FAM nanocrystals in PBS buffer solution at pH = 7.4 (A) and pH = 5 (B).
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f9: Drug release profiles for ZIF-8/5-FU@FA-CHI-5-FAM nanocrystals in PBS buffer solution at pH = 7.4 (A) and pH = 5 (B).

Mentions: For the efficient and effective delivery of 5-FU, we designed a multifunctional hollow MOF that exhibits targeting ability and a high drug loading level. Here, the 5-FU encapsulation efficiency on ZIF-8/5-FU@FA-CHI-5-FAM is 51%, which is higher than the 32% encapsulation efficiency for the original ZIF-8. The large specific surface area of the hollow ZIF-8 is reasonably regarded as a cause of the high drug loadings. The BET surface area of the hollow ZIF-8 is as high as 1596 m2/g, whereas the BET surface area of solid ZIF-8 is only 136.7 m2/g (Supplementary Figures S9 and S10). Additionally, the controlled release of 5-FU from ZIF-8/5-FU@FA-CHI-5-FAM is conducted in PBS buffer solutions (pH = 7.4 and pH = 5) at 37 °C, and the drug delivery profiles are shown in Fig. 9. The concentration of 5-FU released from ZIF-8/5-FU@FA-CHI-5-FAM is obtained according to the standard equation. The UV-vis absorption spectra of 5-FU at different concentrations in PBS buffer solution and the standard 5-FU concentration curve are shown in Supplementary Figures S10 and S11, respectively. Through the curve fitting, we calculated the standard equation of 5-FU as A = 0.00186c + 0.06753, R2 = 0.99994, where A is the absorbance, c is the concentration of 5-FU solution and R is the correlation coefficient.


Fabrication of functional hollow microspheres constructed from MOF shells: Promising drug delivery systems with high loading capacity and targeted transport
Drug release profiles for ZIF-8/5-FU@FA-CHI-5-FAM nanocrystals in PBS buffer solution at pH = 7.4 (A) and pH = 5 (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120282&req=5

f9: Drug release profiles for ZIF-8/5-FU@FA-CHI-5-FAM nanocrystals in PBS buffer solution at pH = 7.4 (A) and pH = 5 (B).
Mentions: For the efficient and effective delivery of 5-FU, we designed a multifunctional hollow MOF that exhibits targeting ability and a high drug loading level. Here, the 5-FU encapsulation efficiency on ZIF-8/5-FU@FA-CHI-5-FAM is 51%, which is higher than the 32% encapsulation efficiency for the original ZIF-8. The large specific surface area of the hollow ZIF-8 is reasonably regarded as a cause of the high drug loadings. The BET surface area of the hollow ZIF-8 is as high as 1596 m2/g, whereas the BET surface area of solid ZIF-8 is only 136.7 m2/g (Supplementary Figures S9 and S10). Additionally, the controlled release of 5-FU from ZIF-8/5-FU@FA-CHI-5-FAM is conducted in PBS buffer solutions (pH = 7.4 and pH = 5) at 37 °C, and the drug delivery profiles are shown in Fig. 9. The concentration of 5-FU released from ZIF-8/5-FU@FA-CHI-5-FAM is obtained according to the standard equation. The UV-vis absorption spectra of 5-FU at different concentrations in PBS buffer solution and the standard 5-FU concentration curve are shown in Supplementary Figures S10 and S11, respectively. Through the curve fitting, we calculated the standard equation of 5-FU as A = 0.00186c + 0.06753, R2 = 0.99994, where A is the absorbance, c is the concentration of 5-FU solution and R is the correlation coefficient.

View Article: PubMed Central - PubMed

ABSTRACT

An advanced multifunctional, hollow metal-organic framework (MOF) drug delivery system with a high drug loading level and targeted delivery was designed and fabricated for the first time and applied to inhibit tumour cell growth. This hollow MOF targeting drug delivery system was prepared via a simple post-synthetic surface modification procedure, starting from hollow ZIF-8 successfully obtained for the first time via a mild phase transformation under solvothermal conditions. As a result, the hollow ZIF-8 exhibits a higher loading capacity for the model anticancer drug 5-fluorouracil (5-FU). Subsequently, 5-FU-loaded ZIF-8 was encapsulated into polymer layers (FA-CHI-5-FAM) with three components: a chitosan (CHI) backbone, the imaging agent 5-carboxyfluorescein (5-FAM), and the targeting reagent folic acid (FA). Thus, an advanced drug delivery system, ZIF-8/5-FU@FA-CHI-5-FAM, was fabricated. A cell imaging assay demonstrated that ZIF-8/5-FU@FA-CHI-5-FAM could target and be taken up by MGC-803 cells. Furthermore, the as-prepared ZIF-8/5-FU@FA-CHI-5-FAM exhibited stronger cell growth inhibitory effects on MGC-803 cells because of the release of 5-FU, as confirmed by a cell viability assay. In addition, a drug release experiment in vitro indicated that ZIF-8/5-FU@FA-CHI-5-FAM exhibited high loading capacity (51%) and a sustained drug release behaviour. Therefore, ZIF-8/5-FU@FA-CHI-5-FAM could provide targeted drug transportation, imaging tracking and localized sustained release.

No MeSH data available.