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Fabrication of functional hollow microspheres constructed from MOF shells: Promising drug delivery systems with high loading capacity and targeted transport

View Article: PubMed Central - PubMed

ABSTRACT

An advanced multifunctional, hollow metal-organic framework (MOF) drug delivery system with a high drug loading level and targeted delivery was designed and fabricated for the first time and applied to inhibit tumour cell growth. This hollow MOF targeting drug delivery system was prepared via a simple post-synthetic surface modification procedure, starting from hollow ZIF-8 successfully obtained for the first time via a mild phase transformation under solvothermal conditions. As a result, the hollow ZIF-8 exhibits a higher loading capacity for the model anticancer drug 5-fluorouracil (5-FU). Subsequently, 5-FU-loaded ZIF-8 was encapsulated into polymer layers (FA-CHI-5-FAM) with three components: a chitosan (CHI) backbone, the imaging agent 5-carboxyfluorescein (5-FAM), and the targeting reagent folic acid (FA). Thus, an advanced drug delivery system, ZIF-8/5-FU@FA-CHI-5-FAM, was fabricated. A cell imaging assay demonstrated that ZIF-8/5-FU@FA-CHI-5-FAM could target and be taken up by MGC-803 cells. Furthermore, the as-prepared ZIF-8/5-FU@FA-CHI-5-FAM exhibited stronger cell growth inhibitory effects on MGC-803 cells because of the release of 5-FU, as confirmed by a cell viability assay. In addition, a drug release experiment in vitro indicated that ZIF-8/5-FU@FA-CHI-5-FAM exhibited high loading capacity (51%) and a sustained drug release behaviour. Therefore, ZIF-8/5-FU@FA-CHI-5-FAM could provide targeted drug transportation, imaging tracking and localized sustained release.

No MeSH data available.


Viabilities of MGC-803 cells in the presence of ZIF-8 @ FA-CHI-5-FAM (A), ZIF-8/5-FU@FA-CHI-5-FAM (B) and 5-FU (C), as assayed by MTT.
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f8: Viabilities of MGC-803 cells in the presence of ZIF-8 @ FA-CHI-5-FAM (A), ZIF-8/5-FU@FA-CHI-5-FAM (B) and 5-FU (C), as assayed by MTT.

Mentions: In vitro cell viabilities of ZIF-8@ FA-CHI-5-FAM (Fig. 8A), ZIF-8/5-FU@FA-CHI-5-FAM (Fig. 8B) and free 5-FU (Fig. 8C) on MGC-803 cells are evaluated by MTT assay to study the bio-toxicity of ZIF-8@ FA-CHI-5-FAM and the therapeutic effect of ZIF-8/5-FU@FA-CHI-5-FAM. After 48 h incubation with MGC-803 cells, the MGC-803 cells treated with ZIF-8@ FA-CHI-5-FAM show no obvious toxicity when the concentration of the ZIF-8@FA-CHI-5-FAM is 200 μg/mL. However, ZIF-8/5-FU@FA-CHI-5-FAM exhibits a stronger cell growth inhibition effect on MGC-803 cells compared to ZIF-8@FA-CHI-5-FAM at the same concentration. Only 200 μg/mL of ZIF-8/5-FU@FA-CHI-5-FAM induces 55% death of the MGC-803 cells. Additionally, the cytotoxicity of ZIF-8/5-FU@FA-CHI-5-FAM is reduced against MGC-803 cells compared to the cytotoxicity of 5-FU alone, which is attributed to the lower amount of 5-FU contained in ZIF-8/5-FU@FA-CHI-5-FAM. Thus, we conclude that 5-FU can release from ZIF-8/5-FU@FA-CHI-5-FAM and induce cell death, which indicates that the ZIF-8/5-FU@FA-CHI-5-FAM can be potentially applied as an efficient drug-delivery system for cancer treatment.


Fabrication of functional hollow microspheres constructed from MOF shells: Promising drug delivery systems with high loading capacity and targeted transport
Viabilities of MGC-803 cells in the presence of ZIF-8 @ FA-CHI-5-FAM (A), ZIF-8/5-FU@FA-CHI-5-FAM (B) and 5-FU (C), as assayed by MTT.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120282&req=5

f8: Viabilities of MGC-803 cells in the presence of ZIF-8 @ FA-CHI-5-FAM (A), ZIF-8/5-FU@FA-CHI-5-FAM (B) and 5-FU (C), as assayed by MTT.
Mentions: In vitro cell viabilities of ZIF-8@ FA-CHI-5-FAM (Fig. 8A), ZIF-8/5-FU@FA-CHI-5-FAM (Fig. 8B) and free 5-FU (Fig. 8C) on MGC-803 cells are evaluated by MTT assay to study the bio-toxicity of ZIF-8@ FA-CHI-5-FAM and the therapeutic effect of ZIF-8/5-FU@FA-CHI-5-FAM. After 48 h incubation with MGC-803 cells, the MGC-803 cells treated with ZIF-8@ FA-CHI-5-FAM show no obvious toxicity when the concentration of the ZIF-8@FA-CHI-5-FAM is 200 μg/mL. However, ZIF-8/5-FU@FA-CHI-5-FAM exhibits a stronger cell growth inhibition effect on MGC-803 cells compared to ZIF-8@FA-CHI-5-FAM at the same concentration. Only 200 μg/mL of ZIF-8/5-FU@FA-CHI-5-FAM induces 55% death of the MGC-803 cells. Additionally, the cytotoxicity of ZIF-8/5-FU@FA-CHI-5-FAM is reduced against MGC-803 cells compared to the cytotoxicity of 5-FU alone, which is attributed to the lower amount of 5-FU contained in ZIF-8/5-FU@FA-CHI-5-FAM. Thus, we conclude that 5-FU can release from ZIF-8/5-FU@FA-CHI-5-FAM and induce cell death, which indicates that the ZIF-8/5-FU@FA-CHI-5-FAM can be potentially applied as an efficient drug-delivery system for cancer treatment.

View Article: PubMed Central - PubMed

ABSTRACT

An advanced multifunctional, hollow metal-organic framework (MOF) drug delivery system with a high drug loading level and targeted delivery was designed and fabricated for the first time and applied to inhibit tumour cell growth. This hollow MOF targeting drug delivery system was prepared via a simple post-synthetic surface modification procedure, starting from hollow ZIF-8 successfully obtained for the first time via a mild phase transformation under solvothermal conditions. As a result, the hollow ZIF-8 exhibits a higher loading capacity for the model anticancer drug 5-fluorouracil (5-FU). Subsequently, 5-FU-loaded ZIF-8 was encapsulated into polymer layers (FA-CHI-5-FAM) with three components: a chitosan (CHI) backbone, the imaging agent 5-carboxyfluorescein (5-FAM), and the targeting reagent folic acid (FA). Thus, an advanced drug delivery system, ZIF-8/5-FU@FA-CHI-5-FAM, was fabricated. A cell imaging assay demonstrated that ZIF-8/5-FU@FA-CHI-5-FAM could target and be taken up by MGC-803 cells. Furthermore, the as-prepared ZIF-8/5-FU@FA-CHI-5-FAM exhibited stronger cell growth inhibitory effects on MGC-803 cells because of the release of 5-FU, as confirmed by a cell viability assay. In addition, a drug release experiment in vitro indicated that ZIF-8/5-FU@FA-CHI-5-FAM exhibited high loading capacity (51%) and a sustained drug release behaviour. Therefore, ZIF-8/5-FU@FA-CHI-5-FAM could provide targeted drug transportation, imaging tracking and localized sustained release.

No MeSH data available.