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Evaluation of Lethal Giant Larvae as a Schistosomiasis Vaccine Candidate

View Article: PubMed Central - PubMed

ABSTRACT

Schistosomiasis is a neglected tropical disease of humans, and it is considered to be the second most devastating parasitic disease after malaria. Eggs produced by normally developed female worms are important in the transmission of the parasite, and they responsible for the pathogenesis of schistosomiasis. The tumor suppressor gene lethal giant larvae (lgl) has an essential function in establishing apical-basal cell polarity, cell proliferation, differentiation, and tissue organization. In our earlier study, downregulation of the lgl gene induced a significant reduction in the egg hatching rate of Schistosoma japonicum (Sj) eggs. In this study, the Sjlgl gene was used as a vaccine candidate against schistosomiasis, and vaccination achieved and maintained a stable reduction of the egg hatching rate, which is consistent with previous studies, in addition to reducing the worm burden and liver egg burden in some trials.

No MeSH data available.


The selected protein sequence of SjLGL in relation to S. mansoni, M. musculus, and H. sapiens LGL sequences. ClustalX alignment of the derived amino acid sequences of SmLGL (XP_002577079.1), MmLGL (NP_001152876), and HsLGL (NP_004131.3). Regions with a high level of identity and similarity between the LGL sequences are shown in color.
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fig1: The selected protein sequence of SjLGL in relation to S. mansoni, M. musculus, and H. sapiens LGL sequences. ClustalX alignment of the derived amino acid sequences of SmLGL (XP_002577079.1), MmLGL (NP_001152876), and HsLGL (NP_004131.3). Regions with a high level of identity and similarity between the LGL sequences are shown in color.

Mentions: The 849 bp sequence of the selected Sjlgl segment encodes a protein of 282 amino acid residues, with a predicted molecular mass of ~30.76 kDa and a pI of 6.85. A comparison of the amino acid sequences showed that the LGL segment of S. japonicum that was selected in our experiment shared 86%, 36%, and 33% identity with its orthologs in Schistosoma mansoni, Mus musculus, and Homo sapiens, respectively (Figure 1).


Evaluation of Lethal Giant Larvae as a Schistosomiasis Vaccine Candidate
The selected protein sequence of SjLGL in relation to S. mansoni, M. musculus, and H. sapiens LGL sequences. ClustalX alignment of the derived amino acid sequences of SmLGL (XP_002577079.1), MmLGL (NP_001152876), and HsLGL (NP_004131.3). Regions with a high level of identity and similarity between the LGL sequences are shown in color.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5120214&req=5

fig1: The selected protein sequence of SjLGL in relation to S. mansoni, M. musculus, and H. sapiens LGL sequences. ClustalX alignment of the derived amino acid sequences of SmLGL (XP_002577079.1), MmLGL (NP_001152876), and HsLGL (NP_004131.3). Regions with a high level of identity and similarity between the LGL sequences are shown in color.
Mentions: The 849 bp sequence of the selected Sjlgl segment encodes a protein of 282 amino acid residues, with a predicted molecular mass of ~30.76 kDa and a pI of 6.85. A comparison of the amino acid sequences showed that the LGL segment of S. japonicum that was selected in our experiment shared 86%, 36%, and 33% identity with its orthologs in Schistosoma mansoni, Mus musculus, and Homo sapiens, respectively (Figure 1).

View Article: PubMed Central - PubMed

ABSTRACT

Schistosomiasis is a neglected tropical disease of humans, and it is considered to be the second most devastating parasitic disease after malaria. Eggs produced by normally developed female worms are important in the transmission of the parasite, and they responsible for the pathogenesis of schistosomiasis. The tumor suppressor gene lethal giant larvae (lgl) has an essential function in establishing apical-basal cell polarity, cell proliferation, differentiation, and tissue organization. In our earlier study, downregulation of the lgl gene induced a significant reduction in the egg hatching rate of Schistosoma japonicum (Sj) eggs. In this study, the Sjlgl gene was used as a vaccine candidate against schistosomiasis, and vaccination achieved and maintained a stable reduction of the egg hatching rate, which is consistent with previous studies, in addition to reducing the worm burden and liver egg burden in some trials.

No MeSH data available.