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NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF- κ B Signaling Pathway

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the present study is to investigate the protective effects and relevant mechanisms exerted by NEMO-binding domain peptide (NBD) against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. The ALI model was induced by intratracheally administered atomized LPS (5 mg/kg) to BABL/c mice. Half an hour before LPS administration, we treated the mice with increasing concentrations of intratracheally administered NBD or saline aerosol. Two hours after LPS administration, each group of mice was sacrificed. We observed that NBD pretreatment significantly attenuated LPS-induced lung histopathological injury in a dose-dependent manner. Western blotting established that NBD pretreatment obviously attenuated LPS-induced IκB-α and NF-κBp65 activation and NOX1, NOX2, and NOX4 overexpression. Furthermore, NBD pretreatment increased SOD and T-AOC activity and decreased MDA levels in lung tissue. In addition, NBD also inhibited TNF-α and IL-1β secretion in BALF after LPS challenge. In conclusion, NBD protects against LPS-induced ALI in mice.

No MeSH data available.


Related in: MedlinePlus

Effects of NBD on pulmonary vascular leakage in mice with ALI. BALF protein concentrations were assessed to evaluate pulmonary vascular leakage. The data indicated that NBD can noticeably reduce BALF protein levels. Data are expressed as the mean ± SD (n = 6). A represents versus control group, AP < 0.05; B represents versus model group, BP < 0.05; C represents versus N-NBD group, CP < 0.05; D  represents versus NBD-2 group, DP < 0.05.
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fig2: Effects of NBD on pulmonary vascular leakage in mice with ALI. BALF protein concentrations were assessed to evaluate pulmonary vascular leakage. The data indicated that NBD can noticeably reduce BALF protein levels. Data are expressed as the mean ± SD (n = 6). A represents versus control group, AP < 0.05; B represents versus model group, BP < 0.05; C represents versus N-NBD group, CP < 0.05; D  represents versus NBD-2 group, DP < 0.05.

Mentions: Uncontrolled inflammation causes vascular leakage in the lung in CLP-induced acute lung injury. BALF protein levels were assessed at 2 h after LPS injection to evaluate the effects of NBD on alveolar-capillary membrane barrier integrity and pulmonary vascular leakage. The results indicated that the total protein concentration in the BALF was increased after LPS administration. However, the total protein concentration was markedly decreased in a dose-dependent manner in the NBD group compared with the ALI group (P < 0.05) (Figure 2). The total numbers of cells and neutrophils in the BALF were also quantified at 2 h after LPS administration. The results indicated that the total numbers of cells and neutrophils in the BALF were significantly increased in the ALI group compared to the control group (P < 0.05). Similar to the above results, NBD treatment significantly decreased the total numbers of cells and neutrophils in the BALF (P < 0.05) (Figure 3).


NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF- κ B Signaling Pathway
Effects of NBD on pulmonary vascular leakage in mice with ALI. BALF protein concentrations were assessed to evaluate pulmonary vascular leakage. The data indicated that NBD can noticeably reduce BALF protein levels. Data are expressed as the mean ± SD (n = 6). A represents versus control group, AP < 0.05; B represents versus model group, BP < 0.05; C represents versus N-NBD group, CP < 0.05; D  represents versus NBD-2 group, DP < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
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fig2: Effects of NBD on pulmonary vascular leakage in mice with ALI. BALF protein concentrations were assessed to evaluate pulmonary vascular leakage. The data indicated that NBD can noticeably reduce BALF protein levels. Data are expressed as the mean ± SD (n = 6). A represents versus control group, AP < 0.05; B represents versus model group, BP < 0.05; C represents versus N-NBD group, CP < 0.05; D  represents versus NBD-2 group, DP < 0.05.
Mentions: Uncontrolled inflammation causes vascular leakage in the lung in CLP-induced acute lung injury. BALF protein levels were assessed at 2 h after LPS injection to evaluate the effects of NBD on alveolar-capillary membrane barrier integrity and pulmonary vascular leakage. The results indicated that the total protein concentration in the BALF was increased after LPS administration. However, the total protein concentration was markedly decreased in a dose-dependent manner in the NBD group compared with the ALI group (P < 0.05) (Figure 2). The total numbers of cells and neutrophils in the BALF were also quantified at 2 h after LPS administration. The results indicated that the total numbers of cells and neutrophils in the BALF were significantly increased in the ALI group compared to the control group (P < 0.05). Similar to the above results, NBD treatment significantly decreased the total numbers of cells and neutrophils in the BALF (P < 0.05) (Figure 3).

View Article: PubMed Central - PubMed

ABSTRACT

The aim of the present study is to investigate the protective effects and relevant mechanisms exerted by NEMO-binding domain peptide (NBD) against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. The ALI model was induced by intratracheally administered atomized LPS (5&thinsp;mg/kg) to BABL/c mice. Half an hour before LPS administration, we treated the mice with increasing concentrations of intratracheally administered NBD or saline aerosol. Two hours after LPS administration, each group of mice was sacrificed. We observed that NBD pretreatment significantly attenuated LPS-induced lung histopathological injury in a dose-dependent manner. Western blotting established that NBD pretreatment obviously attenuated LPS-induced I&kappa;B-&alpha; and NF-&kappa;Bp65 activation and NOX1, NOX2, and NOX4 overexpression. Furthermore, NBD pretreatment increased SOD and T-AOC activity and decreased MDA levels in lung tissue. In addition, NBD also inhibited TNF-&alpha; and IL-1&beta; secretion in BALF after LPS challenge. In conclusion, NBD protects against LPS-induced ALI in mice.

No MeSH data available.


Related in: MedlinePlus