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Serum Levels of Soluble ST2 and IL-10 Are Associated with Disease Severity in Patients with IgA Nephropathy

View Article: PubMed Central - PubMed

ABSTRACT

Background. The IL-33/ST2 axis is involved in humoral immune responses. Method. The concentrations of sera IL-33 and sST2 in 74 patients and 34 healthy controls (HC) were measured by ELISA. Clinical and laboratory data were examined. The potential association between sera IL-33 and sST2 and the clinical parameters in IgAN patients were analyzed. Results. No difference was discovered in IL-33 concentrations between IgAN patients and HCs; however, the sST2 were significantly higher in each stage of IgAN progression than in the HC. The concentration of sST2 was positively correlated with IL-33 levels in IgAN patients. Higher levels of sera IL-2, IL-4, IL-10, IL-17A, and IFN-γ were detected in patients compared to the HC. The concentration of serum sST2 was positively correlated with the levels of IL-10 in IgAN patients. Furthermore, serum sST2 was negatively correlated with the values of eGFR and serum calcium. Serum sST2 was positively correlated with 24-hour urinary protein, serum phosphorus, and serum IgA; however, serum IL-33 was not associated with these. Following treatment, serum sST2 was significantly decreased, while sera IL-4 and IL-10 were significantly increased. Conclusions. Increased sST2 and IL-10 but not IL-33 may be involved in the pathogenic process of IgAN.

No MeSH data available.


Increased serum levels of IL-2, IL-4, IL-10, IL-17A, and IFN-γ in IgAN patients before treatment. CBA was used to detect the levels of sera IL-2, IL-4, IL-17A, IL-10, and IFN-γ ((a)–(e), resp.) in IgAN patients and HCs. Data are expressed as mean values of individual samples from two divided experiments. Horizontal lines represent median values of different groups.
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fig4: Increased serum levels of IL-2, IL-4, IL-10, IL-17A, and IFN-γ in IgAN patients before treatment. CBA was used to detect the levels of sera IL-2, IL-4, IL-17A, IL-10, and IFN-γ ((a)–(e), resp.) in IgAN patients and HCs. Data are expressed as mean values of individual samples from two divided experiments. Horizontal lines represent median values of different groups.

Mentions: To explore the potential function of IL-2, IL-4, IL-10, IL-17A, and IFN-γ in IgAN, we measured the serum levels by cytometric bead array (CBA). We found that the concentrations of sera IL-2, IL-4, IL-10, IL-17A, and IFN-γ were significantly increased in IgAN patients compared to the HCs (p < 0.05, Figures 4(a)–4(e)). No apparent differences in the concentrations of those cytokines were discovered among the different stages of IgAN patients (data not shown). Our data indicates that those cytokines may participate in the pathogenesis of IgAN.


Serum Levels of Soluble ST2 and IL-10 Are Associated with Disease Severity in Patients with IgA Nephropathy
Increased serum levels of IL-2, IL-4, IL-10, IL-17A, and IFN-γ in IgAN patients before treatment. CBA was used to detect the levels of sera IL-2, IL-4, IL-17A, IL-10, and IFN-γ ((a)–(e), resp.) in IgAN patients and HCs. Data are expressed as mean values of individual samples from two divided experiments. Horizontal lines represent median values of different groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC5120178&req=5

fig4: Increased serum levels of IL-2, IL-4, IL-10, IL-17A, and IFN-γ in IgAN patients before treatment. CBA was used to detect the levels of sera IL-2, IL-4, IL-17A, IL-10, and IFN-γ ((a)–(e), resp.) in IgAN patients and HCs. Data are expressed as mean values of individual samples from two divided experiments. Horizontal lines represent median values of different groups.
Mentions: To explore the potential function of IL-2, IL-4, IL-10, IL-17A, and IFN-γ in IgAN, we measured the serum levels by cytometric bead array (CBA). We found that the concentrations of sera IL-2, IL-4, IL-10, IL-17A, and IFN-γ were significantly increased in IgAN patients compared to the HCs (p < 0.05, Figures 4(a)–4(e)). No apparent differences in the concentrations of those cytokines were discovered among the different stages of IgAN patients (data not shown). Our data indicates that those cytokines may participate in the pathogenesis of IgAN.

View Article: PubMed Central - PubMed

ABSTRACT

Background. The IL-33/ST2 axis is involved in humoral immune responses. Method. The concentrations of sera IL-33 and sST2 in 74 patients and 34 healthy controls (HC) were measured by ELISA. Clinical and laboratory data were examined. The potential association between sera IL-33 and sST2 and the clinical parameters in IgAN patients were analyzed. Results. No difference was discovered in IL-33 concentrations between IgAN patients and HCs; however, the sST2 were significantly higher in each stage of IgAN progression than in the HC. The concentration of sST2 was positively correlated with IL-33 levels in IgAN patients. Higher levels of sera IL-2, IL-4, IL-10, IL-17A, and IFN-&gamma; were detected in patients compared to the HC. The concentration of serum sST2 was positively correlated with the levels of IL-10 in IgAN patients. Furthermore, serum sST2 was negatively correlated with the values of eGFR and serum calcium. Serum sST2 was positively correlated with 24-hour urinary protein, serum phosphorus, and serum IgA; however, serum IL-33 was not associated with these. Following treatment, serum sST2 was significantly decreased, while sera IL-4 and IL-10 were significantly increased. Conclusions. Increased sST2 and IL-10 but not IL-33 may be involved in the pathogenic process of IgAN.

No MeSH data available.