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First Case of Actinomycetoma in France Due to a Novel Nocardia Species, Nocardia boironii sp. nov.

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ABSTRACT

Bacterial mycetoma is an endemic infection in areas with tropical and subtropical climates. Thus, its presence in temperate climate areas remains rare. We report here the first case of autochthonous actinomycetoma in continental France originating from a Nocardia species other than N. brasiliensis, namely, Nocardia boironii. Considering the history of the patient, the infection source of strain OFN 14.177T may be from frequent contact with the soil over many years because of his gardening activities. The discovery of a French autochthonous Nocardia species responsible for actinomycetoma reveals the importance of considering the possibility of having autochthonous infections of this type in nontropical countries, not only imported cases from tropical countries. However, further studies are needed to elucidate the real incidence of this new species.

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(a) DNA-DNA hybridizations of strain OFN 14.177T with the species having the highest similarity percentages in the 16S rRNA, hsp65, sod, and secA1 genes. (b) Susceptibility of strain OFN 14.177T and type strains of genetically closest species against antibiotics. The MIC (CMI) break points were obtained from reference 42 except for those for tigecycline (43). -, no data. (c) Similarity percentages of the sequences of the rRNA 16S, hsp65, sod, and secA1 genes between strain OFN 14.177T and the phylogenetically closest species. The highest similarity values for each gene are indicated in boldface type. (d to f) Physiological characteristics of the OFN 14.177T strain and Nocardia reference type strains (strain 1, N. boironii sp. nov. OFN 14.177T; strain 2, N. altamirensis DSM 44997T; strain 3, N. vulneris DSM 45737T; strain 4, N. brasiliensis ATCC 19296T; strain 5, N. iowensis DSM 45197T; strain 6, N. tenerifensis DSM 44704T). Growth in carbon source and decomposition are shown as percentages (wt/vol). Reactions: −, negative; +, positive; W, weak.
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fig2: (a) DNA-DNA hybridizations of strain OFN 14.177T with the species having the highest similarity percentages in the 16S rRNA, hsp65, sod, and secA1 genes. (b) Susceptibility of strain OFN 14.177T and type strains of genetically closest species against antibiotics. The MIC (CMI) break points were obtained from reference 42 except for those for tigecycline (43). -, no data. (c) Similarity percentages of the sequences of the rRNA 16S, hsp65, sod, and secA1 genes between strain OFN 14.177T and the phylogenetically closest species. The highest similarity values for each gene are indicated in boldface type. (d to f) Physiological characteristics of the OFN 14.177T strain and Nocardia reference type strains (strain 1, N. boironii sp. nov. OFN 14.177T; strain 2, N. altamirensis DSM 44997T; strain 3, N. vulneris DSM 45737T; strain 4, N. brasiliensis ATCC 19296T; strain 5, N. iowensis DSM 45197T; strain 6, N. tenerifensis DSM 44704T). Growth in carbon source and decomposition are shown as percentages (wt/vol). Reactions: −, negative; +, positive; W, weak.

Mentions: An aerobic, Gram-positive bacterium, positive for catalase and presenting filamentous thin branched bacilli that easily fragment into bacillus- or coccus-shaped elements. The color of the mycelium is yellow-orange. The aerial mycelium is white with a nonhomogeneous distribution. The colonies measure 1 to 3 mm in diameter on Bennett agar. Growth occurs at 28°C and 37°C, but not at 45°C. Major fatty acids include palmitic acid (C16:0) (46.69%), tuberculostearic acid (C10-Me-18:0) (22.86%), and oleic acid (C18:1w9c) (12.29%). The mycolic acids are 46 to 60 carbon atoms in length (the main mycolic acids have a chain length of 52 and 54 carbon atoms). The DNA G+C content of the type strain is 68.2 mol%. Physiological and biochemical characteristics of strain OFN 14.177T are presented in Fig. 2d, e, and f.


First Case of Actinomycetoma in France Due to a Novel Nocardia Species, Nocardia boironii sp. nov.
(a) DNA-DNA hybridizations of strain OFN 14.177T with the species having the highest similarity percentages in the 16S rRNA, hsp65, sod, and secA1 genes. (b) Susceptibility of strain OFN 14.177T and type strains of genetically closest species against antibiotics. The MIC (CMI) break points were obtained from reference 42 except for those for tigecycline (43). -, no data. (c) Similarity percentages of the sequences of the rRNA 16S, hsp65, sod, and secA1 genes between strain OFN 14.177T and the phylogenetically closest species. The highest similarity values for each gene are indicated in boldface type. (d to f) Physiological characteristics of the OFN 14.177T strain and Nocardia reference type strains (strain 1, N. boironii sp. nov. OFN 14.177T; strain 2, N. altamirensis DSM 44997T; strain 3, N. vulneris DSM 45737T; strain 4, N. brasiliensis ATCC 19296T; strain 5, N. iowensis DSM 45197T; strain 6, N. tenerifensis DSM 44704T). Growth in carbon source and decomposition are shown as percentages (wt/vol). Reactions: −, negative; +, positive; W, weak.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5120171&req=5

fig2: (a) DNA-DNA hybridizations of strain OFN 14.177T with the species having the highest similarity percentages in the 16S rRNA, hsp65, sod, and secA1 genes. (b) Susceptibility of strain OFN 14.177T and type strains of genetically closest species against antibiotics. The MIC (CMI) break points were obtained from reference 42 except for those for tigecycline (43). -, no data. (c) Similarity percentages of the sequences of the rRNA 16S, hsp65, sod, and secA1 genes between strain OFN 14.177T and the phylogenetically closest species. The highest similarity values for each gene are indicated in boldface type. (d to f) Physiological characteristics of the OFN 14.177T strain and Nocardia reference type strains (strain 1, N. boironii sp. nov. OFN 14.177T; strain 2, N. altamirensis DSM 44997T; strain 3, N. vulneris DSM 45737T; strain 4, N. brasiliensis ATCC 19296T; strain 5, N. iowensis DSM 45197T; strain 6, N. tenerifensis DSM 44704T). Growth in carbon source and decomposition are shown as percentages (wt/vol). Reactions: −, negative; +, positive; W, weak.
Mentions: An aerobic, Gram-positive bacterium, positive for catalase and presenting filamentous thin branched bacilli that easily fragment into bacillus- or coccus-shaped elements. The color of the mycelium is yellow-orange. The aerial mycelium is white with a nonhomogeneous distribution. The colonies measure 1 to 3 mm in diameter on Bennett agar. Growth occurs at 28°C and 37°C, but not at 45°C. Major fatty acids include palmitic acid (C16:0) (46.69%), tuberculostearic acid (C10-Me-18:0) (22.86%), and oleic acid (C18:1w9c) (12.29%). The mycolic acids are 46 to 60 carbon atoms in length (the main mycolic acids have a chain length of 52 and 54 carbon atoms). The DNA G+C content of the type strain is 68.2 mol%. Physiological and biochemical characteristics of strain OFN 14.177T are presented in Fig. 2d, e, and f.

View Article: PubMed Central - PubMed

ABSTRACT

Bacterial mycetoma is an endemic infection in areas with tropical and subtropical climates. Thus, its presence in temperate climate areas remains rare. We report here the first case of autochthonous actinomycetoma in continental France originating from a Nocardia species other than N. brasiliensis, namely, Nocardia boironii. Considering the history of the patient, the infection source of strain OFN 14.177T may be from frequent contact with the soil over many years because of his gardening activities. The discovery of a French autochthonous Nocardia species responsible for actinomycetoma reveals the importance of considering the possibility of having autochthonous infections of this type in nontropical countries, not only imported cases from tropical countries. However, further studies are needed to elucidate the real incidence of this new species.

No MeSH data available.


Related in: MedlinePlus