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Overexpression of TaNAC2D Displays Opposite Responses to Abiotic Stresses between Seedling and Mature Stage of Transgenic Arabidopsis

View Article: PubMed Central - PubMed

ABSTRACT

Environmental stresses frequently affect plant growth and development, and many genes have been found to be induced by unfavorable environmental conditions. Here, we reported the biological functions of TaNAC2D, a stress-related NAC (NAM, ATAF, and CUC) gene from wheat. TaNAC2D showed transcriptional activator activity in yeast. TaNAC2D-GFP fusion protein was localized in the nucleus of wheat mesophyll protoplasts. TaNAC2D transcript abundance was significantly induced by NaCl, PEG6000, and abscisic acid (ABA) at seedling stage, and repressed by NaCl and PEG6000 at mature plant stage. When TaNAC2D was introduced into Arabidopsis, the 35-day-old soil-grown TaNAC2D-overexpression (TaNAC2D-OX) plants displayed slower stomatal closure, higher water loss rate, and more sensitivity to salt and drought stresses compared with WT plants. In contrast, TaNAC2D-OX seedlings, grown on 1/2 MS medium supplemented with different concentrations of NaCl, Mannitol, and MV, had enhanced tolerances to salt, osmotic and oxidative stresses during seed germination and post-germination periods. The opposite stress-responsive phenotypes of transgenic Arabidopsis were consistent with the expression patterns of TaNAC2D in wheat. Moreover, under high salinity and dehydration conditions, three marker genes, including NCED3, RD29A, and RD29B, were down-regulated in 35-day-old TaNAC2D-OX plants grown in soil and up-regulated in 14-day-old TaNAC2D-OX seedlings grown on 1/2 MS medium. Our results suggest that the change in growth stages and environmental conditions may regulate TaNAC2D’s function.

No MeSH data available.


Expression patterns of TaNAC2D. The 10-day-old wheat seedlings and the 50-day-old mature wheat plants were treated with NaCl, PEG6000, ABA, and H2O2, transcript levels of TaNAC2D in wheat leaves were determined by qRT-PCR and normalized to TaActin. Data are means ± SE of three biological replicates. Asterisks indicate significant differences from mock (∗P < 0.05, ∗∗P < 0.01).
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Figure 2: Expression patterns of TaNAC2D. The 10-day-old wheat seedlings and the 50-day-old mature wheat plants were treated with NaCl, PEG6000, ABA, and H2O2, transcript levels of TaNAC2D in wheat leaves were determined by qRT-PCR and normalized to TaActin. Data are means ± SE of three biological replicates. Asterisks indicate significant differences from mock (∗P < 0.05, ∗∗P < 0.01).

Mentions: Organ-specific analysis revealed that TaNAC2D transcripts were expressed in all organs except endosperm of wheat, and highly expressed in leaves (Supplementary Figure S3). Moreover, we further examined the expression levels of TaNAC2D under abiotic stress. The qRT-PCR results showed that TaNAC2D was significantly induced by NaCl, PEG, and ABA at seedling stage, and repressed by NaCl and PEG at mature plant stage (Figure 2). These results indicate that the expression of TaNAC2D is regulated at different stages under stress conditions.


Overexpression of TaNAC2D Displays Opposite Responses to Abiotic Stresses between Seedling and Mature Stage of Transgenic Arabidopsis
Expression patterns of TaNAC2D. The 10-day-old wheat seedlings and the 50-day-old mature wheat plants were treated with NaCl, PEG6000, ABA, and H2O2, transcript levels of TaNAC2D in wheat leaves were determined by qRT-PCR and normalized to TaActin. Data are means ± SE of three biological replicates. Asterisks indicate significant differences from mock (∗P < 0.05, ∗∗P < 0.01).
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Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120104&req=5

Figure 2: Expression patterns of TaNAC2D. The 10-day-old wheat seedlings and the 50-day-old mature wheat plants were treated with NaCl, PEG6000, ABA, and H2O2, transcript levels of TaNAC2D in wheat leaves were determined by qRT-PCR and normalized to TaActin. Data are means ± SE of three biological replicates. Asterisks indicate significant differences from mock (∗P < 0.05, ∗∗P < 0.01).
Mentions: Organ-specific analysis revealed that TaNAC2D transcripts were expressed in all organs except endosperm of wheat, and highly expressed in leaves (Supplementary Figure S3). Moreover, we further examined the expression levels of TaNAC2D under abiotic stress. The qRT-PCR results showed that TaNAC2D was significantly induced by NaCl, PEG, and ABA at seedling stage, and repressed by NaCl and PEG at mature plant stage (Figure 2). These results indicate that the expression of TaNAC2D is regulated at different stages under stress conditions.

View Article: PubMed Central - PubMed

ABSTRACT

Environmental stresses frequently affect plant growth and development, and many genes have been found to be induced by unfavorable environmental conditions. Here, we reported the biological functions of TaNAC2D, a stress-related NAC (NAM, ATAF, and CUC) gene from wheat. TaNAC2D showed transcriptional activator activity in yeast. TaNAC2D-GFP fusion protein was localized in the nucleus of wheat mesophyll protoplasts. TaNAC2D transcript abundance was significantly induced by NaCl, PEG6000, and abscisic acid (ABA) at seedling stage, and repressed by NaCl and PEG6000 at mature plant stage. When TaNAC2D was introduced into Arabidopsis, the 35-day-old soil-grown TaNAC2D-overexpression (TaNAC2D-OX) plants displayed slower stomatal closure, higher water loss rate, and more sensitivity to salt and drought stresses compared with WT plants. In contrast, TaNAC2D-OX seedlings, grown on 1/2 MS medium supplemented with different concentrations of NaCl, Mannitol, and MV, had enhanced tolerances to salt, osmotic and oxidative stresses during seed germination and post-germination periods. The opposite stress-responsive phenotypes of transgenic Arabidopsis were consistent with the expression patterns of TaNAC2D in wheat. Moreover, under high salinity and dehydration conditions, three marker genes, including NCED3, RD29A, and RD29B, were down-regulated in 35-day-old TaNAC2D-OX plants grown in soil and up-regulated in 14-day-old TaNAC2D-OX seedlings grown on 1/2 MS medium. Our results suggest that the change in growth stages and environmental conditions may regulate TaNAC2D&rsquo;s function.

No MeSH data available.