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Association between prognostic survival of human colorectal carcinoma and ZNRF3 expression

View Article: PubMed Central - PubMed

ABSTRACT

Purpose: E3 ubiquitin ligase ZNRF3 is linked to the pathogenesis of diseases and tumorigenesis. The present study aims to explore the expression of ZNRF3 and its association with prognostic survival of human colorectal carcinoma.

Methods: A follow-up survey of 168 patients with colorectal carcinoma was performed, and specimens of colorectal tissues were collected for immunohistochemistry and Western blotting analyses. Furthermore, overexpression of ZNRF3 using transient transfection with the recombinant pEGFP-ZNRF3 plasmid and detection of apoptosis and proliferation were performed in HCT-116 cells.

Results: The results showed a diverse feature of ZNRF3 staining, such as strong, moderate, weak, or negative, in colorectal carcinoma tissues. Interestingly, univariate Kaplan–Meier analysis showed that cases with strong or moderate expression of ZNRF3 showed an optimistic disease-free survival and overall survival compared with negative expression of ZNRF3, and multivariate Cox model demonstrated ZNRF3 as an independent prediction index for overall survival and disease-free survival. In vitro, the overexpression of ZNRF3 was related to the negative regulation of Wnt/β-catenin pathway and referred to an induction of apoptosis and suppression of proliferation in ZNRF3-transfected HCT-116 cells.

Conclusion: Our results suggest that the higher expression of ZNRF3 acts as a novel marker of indicating the optimistic prognosis of colorectal carcinoma by suppressing cancer cell growth and facilitating apoptosis.

No MeSH data available.


Distribution of Kaplan–Meier survival functions of OS and DFS in patients with different ZNRF3 expressions.Abbreviations: DFS, disease-free survival; OS, overall survival.
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f3-ott-9-6679: Distribution of Kaplan–Meier survival functions of OS and DFS in patients with different ZNRF3 expressions.Abbreviations: DFS, disease-free survival; OS, overall survival.

Mentions: To evaluate the prognosis significance of ZNRF3 for distant metastasis, Kaplan–Meier curves with a log-rank test for OS and DFS were generated in the 168 patients who accepted radical colectomy. The results showed that patients with ZNRF3 strong positive or moderate positive tumors showed a higher DFS or OS rate compared with patients with ZNRF3-negative tumors (Figure 3; log-rank test, P<0.05). Furthermore, the univariate Cox proportional hazards model showed a significant hazard ratio (HR) of DFS or OS between the ZNRF3 strong positive or moderate positive and ZNRF3-negative groups after surgery (DFS-HR =0.246 or 0.364; OS-HR =0.286 or 0.452, P<0.05; Table 3). In addition, T staging and N staging were correlated with OS and DFS (P<0.05). The further multivariate Cox proportional hazards model revealed that ZNRF3 expression was an independent prognostic marker for tumor recurrence and overall survival (DFS-HR =0.574; OS-HR =0.581, P<0.05; Table 4). Generally, patients with ZNRF3 strong positive or moderate positive tumors had an optimistic DFS and OS compared with patients with ZNRF3-negative tumors.


Association between prognostic survival of human colorectal carcinoma and ZNRF3 expression
Distribution of Kaplan–Meier survival functions of OS and DFS in patients with different ZNRF3 expressions.Abbreviations: DFS, disease-free survival; OS, overall survival.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC5094639&req=5

f3-ott-9-6679: Distribution of Kaplan–Meier survival functions of OS and DFS in patients with different ZNRF3 expressions.Abbreviations: DFS, disease-free survival; OS, overall survival.
Mentions: To evaluate the prognosis significance of ZNRF3 for distant metastasis, Kaplan–Meier curves with a log-rank test for OS and DFS were generated in the 168 patients who accepted radical colectomy. The results showed that patients with ZNRF3 strong positive or moderate positive tumors showed a higher DFS or OS rate compared with patients with ZNRF3-negative tumors (Figure 3; log-rank test, P<0.05). Furthermore, the univariate Cox proportional hazards model showed a significant hazard ratio (HR) of DFS or OS between the ZNRF3 strong positive or moderate positive and ZNRF3-negative groups after surgery (DFS-HR =0.246 or 0.364; OS-HR =0.286 or 0.452, P<0.05; Table 3). In addition, T staging and N staging were correlated with OS and DFS (P<0.05). The further multivariate Cox proportional hazards model revealed that ZNRF3 expression was an independent prognostic marker for tumor recurrence and overall survival (DFS-HR =0.574; OS-HR =0.581, P<0.05; Table 4). Generally, patients with ZNRF3 strong positive or moderate positive tumors had an optimistic DFS and OS compared with patients with ZNRF3-negative tumors.

View Article: PubMed Central - PubMed

ABSTRACT

Purpose: E3 ubiquitin ligase ZNRF3 is linked to the pathogenesis of diseases and tumorigenesis. The present study aims to explore the expression of ZNRF3 and its association with prognostic survival of human colorectal carcinoma.

Methods: A follow-up survey of 168 patients with colorectal carcinoma was performed, and specimens of colorectal tissues were collected for immunohistochemistry and Western blotting analyses. Furthermore, overexpression of ZNRF3 using transient transfection with the recombinant pEGFP-ZNRF3 plasmid and detection of apoptosis and proliferation were performed in HCT-116 cells.

Results: The results showed a diverse feature of ZNRF3 staining, such as strong, moderate, weak, or negative, in colorectal carcinoma tissues. Interestingly, univariate Kaplan&ndash;Meier analysis showed that cases with strong or moderate expression of ZNRF3 showed an optimistic disease-free survival and overall survival compared with negative expression of ZNRF3, and multivariate Cox model demonstrated ZNRF3 as an independent prediction index for overall survival and disease-free survival. In vitro, the overexpression of ZNRF3 was related to the negative regulation of Wnt/&beta;-catenin pathway and referred to an induction of apoptosis and suppression of proliferation in ZNRF3-transfected HCT-116 cells.

Conclusion: Our results suggest that the higher expression of ZNRF3 acts as a novel marker of indicating the optimistic prognosis of colorectal carcinoma by suppressing cancer cell growth and facilitating apoptosis.

No MeSH data available.