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Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus

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ABSTRACT

In this study the effects of phosphate, potassium, yeast extract, and trace metals on the growth of Mucor indicus and chitosan, chitin, and metabolite production by the fungus were investigated. Maximum yield of chitosan (0.32 g/g cell wall) was obtained in a phosphate-free medium. Reversely, cell growth and ethanol formation by the fungus were positively affected in the presence of phosphate. In a phosphate-free medium, the highest chitosan content (0.42 g/g cell wall) and cell growth (0.66 g/g sugar) were obtained at 2.5 g/L of KOH. Potassium concentration had no significant effect on ethanol and glycerol yields. The presence of trace metals significantly increased the chitosan yield at an optimal phosphate and potassium concentration (0.50 g/g cell wall). By contrast, production of ethanol by the fungus was negatively affected (0.33 g/g sugars). A remarkable increase in chitin and decrease in chitosan were observed in the absence of yeast extract and concentrations lower than 2 g/L. The maximum chitosan yield of 51% cell wall was obtained at 5 g/L of yeast extract when the medium contained no phosphate, 2.5 g/L KOH, and 1 mL/L trace metal solution.

No MeSH data available.


Effect of different concentrations of yeast extract on GlcN yield (g/g AIM) (black bars) and GlcNAc yield (white bars) (g/g AIM). Error bars represent the ± standard deviation (SD) of values obtained from independent experiments performed in triplicate. Average SD: p <0.03 (black bars); p <0.015 (white bars).
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ijms-17-01429-f004: Effect of different concentrations of yeast extract on GlcN yield (g/g AIM) (black bars) and GlcNAc yield (white bars) (g/g AIM). Error bars represent the ± standard deviation (SD) of values obtained from independent experiments performed in triplicate. Average SD: p <0.03 (black bars); p <0.015 (white bars).

Mentions: As shown in Table 3, by increasing the biomass production in the presence of yeast extract, the AIM yield was reduced. Maximum AIM yield (0.22 g/g biomass) was obtained at 1 g/L of yeast extract and was 57% more than the minimum yield (0.14 g/g biomass), which occurred at 6 g/L yeast extract. As illustrated in Figure 4, an enhancement in GlcN yield (from 0.08 to 0.51 g/g AIM) was observed by increasing the concentrations of yeast extract (up to 5 g/L). However, GlcN yield was reduced at higher concentrations of yeast extract.


Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus
Effect of different concentrations of yeast extract on GlcN yield (g/g AIM) (black bars) and GlcNAc yield (white bars) (g/g AIM). Error bars represent the ± standard deviation (SD) of values obtained from independent experiments performed in triplicate. Average SD: p <0.03 (black bars); p <0.015 (white bars).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037708&req=5

ijms-17-01429-f004: Effect of different concentrations of yeast extract on GlcN yield (g/g AIM) (black bars) and GlcNAc yield (white bars) (g/g AIM). Error bars represent the ± standard deviation (SD) of values obtained from independent experiments performed in triplicate. Average SD: p <0.03 (black bars); p <0.015 (white bars).
Mentions: As shown in Table 3, by increasing the biomass production in the presence of yeast extract, the AIM yield was reduced. Maximum AIM yield (0.22 g/g biomass) was obtained at 1 g/L of yeast extract and was 57% more than the minimum yield (0.14 g/g biomass), which occurred at 6 g/L yeast extract. As illustrated in Figure 4, an enhancement in GlcN yield (from 0.08 to 0.51 g/g AIM) was observed by increasing the concentrations of yeast extract (up to 5 g/L). However, GlcN yield was reduced at higher concentrations of yeast extract.

View Article: PubMed Central - PubMed

ABSTRACT

In this study the effects of phosphate, potassium, yeast extract, and trace metals on the growth of Mucor indicus and chitosan, chitin, and metabolite production by the fungus were investigated. Maximum yield of chitosan (0.32 g/g cell wall) was obtained in a phosphate-free medium. Reversely, cell growth and ethanol formation by the fungus were positively affected in the presence of phosphate. In a phosphate-free medium, the highest chitosan content (0.42 g/g cell wall) and cell growth (0.66 g/g sugar) were obtained at 2.5 g/L of KOH. Potassium concentration had no significant effect on ethanol and glycerol yields. The presence of trace metals significantly increased the chitosan yield at an optimal phosphate and potassium concentration (0.50 g/g cell wall). By contrast, production of ethanol by the fungus was negatively affected (0.33 g/g sugars). A remarkable increase in chitin and decrease in chitosan were observed in the absence of yeast extract and concentrations lower than 2 g/L. The maximum chitosan yield of 51% cell wall was obtained at 5 g/L of yeast extract when the medium contained no phosphate, 2.5 g/L KOH, and 1 mL/L trace metal solution.

No MeSH data available.