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Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus

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ABSTRACT

In this study the effects of phosphate, potassium, yeast extract, and trace metals on the growth of Mucor indicus and chitosan, chitin, and metabolite production by the fungus were investigated. Maximum yield of chitosan (0.32 g/g cell wall) was obtained in a phosphate-free medium. Reversely, cell growth and ethanol formation by the fungus were positively affected in the presence of phosphate. In a phosphate-free medium, the highest chitosan content (0.42 g/g cell wall) and cell growth (0.66 g/g sugar) were obtained at 2.5 g/L of KOH. Potassium concentration had no significant effect on ethanol and glycerol yields. The presence of trace metals significantly increased the chitosan yield at an optimal phosphate and potassium concentration (0.50 g/g cell wall). By contrast, production of ethanol by the fungus was negatively affected (0.33 g/g sugars). A remarkable increase in chitin and decrease in chitosan were observed in the absence of yeast extract and concentrations lower than 2 g/L. The maximum chitosan yield of 51% cell wall was obtained at 5 g/L of yeast extract when the medium contained no phosphate, 2.5 g/L KOH, and 1 mL/L trace metal solution.

No MeSH data available.


Effect of different potassium hydroxide concentrations on GlcN yield (g/g AIM) (black bars) and GlcNAc yield (g/g AIM) (white bars). Error bars represent the ± standard deviation (SD) of values obtained from independent experiments performed in triplicate. Average SD: p <0.02.
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ijms-17-01429-f002: Effect of different potassium hydroxide concentrations on GlcN yield (g/g AIM) (black bars) and GlcNAc yield (g/g AIM) (white bars). Error bars represent the ± standard deviation (SD) of values obtained from independent experiments performed in triplicate. Average SD: p <0.02.

Mentions: The highest chitin and chitosan levels were obtained in the medium with no phosphoric acid (Figure 1) and ethanol and glycerol yields were relatively high at this condition (Table 1). Therefore, the medium with no phosphoric acid was supplemented with different concentrations of KOH and changes in the yield of the products are summarized in Table 2 and Figure 2. The fungal growth was enhanced by increasing the potassium hydroxide concentration from 0.0 to 2.5 g/L (from 2.44 to 3.32 g/L, respectively). A slightly lower biomass concentration (3.10 g/L) was achieved at 2.88 g/L KOH. Potassium concentration had no significant effect on protein and AIM yields (Table 2).


Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus
Effect of different potassium hydroxide concentrations on GlcN yield (g/g AIM) (black bars) and GlcNAc yield (g/g AIM) (white bars). Error bars represent the ± standard deviation (SD) of values obtained from independent experiments performed in triplicate. Average SD: p <0.02.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037708&req=5

ijms-17-01429-f002: Effect of different potassium hydroxide concentrations on GlcN yield (g/g AIM) (black bars) and GlcNAc yield (g/g AIM) (white bars). Error bars represent the ± standard deviation (SD) of values obtained from independent experiments performed in triplicate. Average SD: p <0.02.
Mentions: The highest chitin and chitosan levels were obtained in the medium with no phosphoric acid (Figure 1) and ethanol and glycerol yields were relatively high at this condition (Table 1). Therefore, the medium with no phosphoric acid was supplemented with different concentrations of KOH and changes in the yield of the products are summarized in Table 2 and Figure 2. The fungal growth was enhanced by increasing the potassium hydroxide concentration from 0.0 to 2.5 g/L (from 2.44 to 3.32 g/L, respectively). A slightly lower biomass concentration (3.10 g/L) was achieved at 2.88 g/L KOH. Potassium concentration had no significant effect on protein and AIM yields (Table 2).

View Article: PubMed Central - PubMed

ABSTRACT

In this study the effects of phosphate, potassium, yeast extract, and trace metals on the growth of Mucor indicus and chitosan, chitin, and metabolite production by the fungus were investigated. Maximum yield of chitosan (0.32 g/g cell wall) was obtained in a phosphate-free medium. Reversely, cell growth and ethanol formation by the fungus were positively affected in the presence of phosphate. In a phosphate-free medium, the highest chitosan content (0.42 g/g cell wall) and cell growth (0.66 g/g sugar) were obtained at 2.5 g/L of KOH. Potassium concentration had no significant effect on ethanol and glycerol yields. The presence of trace metals significantly increased the chitosan yield at an optimal phosphate and potassium concentration (0.50 g/g cell wall). By contrast, production of ethanol by the fungus was negatively affected (0.33 g/g sugars). A remarkable increase in chitin and decrease in chitosan were observed in the absence of yeast extract and concentrations lower than 2 g/L. The maximum chitosan yield of 51% cell wall was obtained at 5 g/L of yeast extract when the medium contained no phosphate, 2.5 g/L KOH, and 1 mL/L trace metal solution.

No MeSH data available.