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Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses

View Article: PubMed Central - PubMed

ABSTRACT

To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-IκB, IκB, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-IκB, Cox-2, and iNOS but up-regulated IκB in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-κB pathway.

No MeSH data available.


The HPLC profile of matrine. (A) sample; (B) solvent.
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ijms-17-01410-f009: The HPLC profile of matrine. (A) sample; (B) solvent.

Mentions: Dried S. flavescens roots were powdered and extracted three times under reflux using 70% aqueous ethanol (each extraction period lasted 1.5 h). The solvent was evaporated under vacuum to yield crude total extracts. Then, the extracts were dissolved in 2% HCl (pH 3.5) and partitioned with ether. The pH of the aqueous solution was re-adjusted with NH4OH to 9.0, and the solution was then extracted with CHCl3. The CHCl3 extract (crude alkaloid) was subjected to silica gel (100–200 mesh) column chromatography and eluted using ethyl acetate-methanol (20:1~1:2) to obtain six fractions, referred to as fractions A–F. Using a series of chromatographic techniques, such as silica gel column chromatography (200–300 mesh) and Sephadex LH-20 chromatography, matrine was isolated from fraction C and identified using NMR (Figure S1). In addition, the purity of the matrine obtained in our study was over 90%, according to the HPLC area normalization method; this result was further confirmed by our findings that the purity factors were within the scope of the threshold value and that the threshold curves did not intersect the purity curves [34] (Figure 9).


Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses
The HPLC profile of matrine. (A) sample; (B) solvent.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037690&req=5

ijms-17-01410-f009: The HPLC profile of matrine. (A) sample; (B) solvent.
Mentions: Dried S. flavescens roots were powdered and extracted three times under reflux using 70% aqueous ethanol (each extraction period lasted 1.5 h). The solvent was evaporated under vacuum to yield crude total extracts. Then, the extracts were dissolved in 2% HCl (pH 3.5) and partitioned with ether. The pH of the aqueous solution was re-adjusted with NH4OH to 9.0, and the solution was then extracted with CHCl3. The CHCl3 extract (crude alkaloid) was subjected to silica gel (100–200 mesh) column chromatography and eluted using ethyl acetate-methanol (20:1~1:2) to obtain six fractions, referred to as fractions A–F. Using a series of chromatographic techniques, such as silica gel column chromatography (200–300 mesh) and Sephadex LH-20 chromatography, matrine was isolated from fraction C and identified using NMR (Figure S1). In addition, the purity of the matrine obtained in our study was over 90%, according to the HPLC area normalization method; this result was further confirmed by our findings that the purity factors were within the scope of the threshold value and that the threshold curves did not intersect the purity curves [34] (Figure 9).

View Article: PubMed Central - PubMed

ABSTRACT

To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-IκB, IκB, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-IκB, Cox-2, and iNOS but up-regulated IκB in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-κB pathway.

No MeSH data available.