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Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses

View Article: PubMed Central - PubMed

ABSTRACT

To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-IκB, IκB, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-IκB, Cox-2, and iNOS but up-regulated IκB in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-κB pathway.

No MeSH data available.


Related in: MedlinePlus

The effects of matrine on the expression of IκB, p-IκB, Cox-2, and iNOS in the synovial tissues of CIA rats. (a) Representative blots of IκB, p-IκB, Cox-2, and iNOS proteins; (b–f) Relative expression levels of IκB, p-IκB, IκB/p-IκB ration, Cox-2, and iNOS. Norm: normal rats; Cont: control rats; Dex: rats treated with dexamethasone. The data are presented as the mean ± SD (n = 4); * p < 0.05, ** p < 0.01, compared with the Cont.
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ijms-17-01410-f008: The effects of matrine on the expression of IκB, p-IκB, Cox-2, and iNOS in the synovial tissues of CIA rats. (a) Representative blots of IκB, p-IκB, Cox-2, and iNOS proteins; (b–f) Relative expression levels of IκB, p-IκB, IκB/p-IκB ration, Cox-2, and iNOS. Norm: normal rats; Cont: control rats; Dex: rats treated with dexamethasone. The data are presented as the mean ± SD (n = 4); * p < 0.05, ** p < 0.01, compared with the Cont.

Mentions: In the present investigation, we also evaluated the protein expression levels of IκB, p-IκB, Cox-2, and iNOS in synovial tissues of CIA rats using western blot assays. As shown in Figure 8, our results indicate that the expression level of IκB was significantly lower in control CIA rats (p < 0.01) than in normal rats. However, the expression levels of p-IκB, Cox-2, and iNOS were markedly up-regulated (p < 0.01) in these rats compared with those levels in normal rats. After treatment with dexamethasone (0.05 mg/kg/days), the expression levels of p-IκB, Cox-2, and iNOS were significantly down-regulated (p < 0.01) and the expression of IκB was elevated (p < 0.01) compared with levels in the control CIA rats. Furthermore, we also found that matrine (80 mg/kg) decreased the expression levels of p-IκB, Cox-2, and iNOS and increased the expression of IκB (p < 0.01) compared with the levels observed in the control CIA rats. Additionally, when administered at a dose of 40 mg/kg, matrine decreased the expression of p-IκB, Cox-2, and iNOS (p < 0.05) but had no effect on the expression of IκB (p > 0.05). Moreover, when administered at a dose of 20 mg/kg, matrine decreased the expression of Cox-2 and iNOS (p < 0.05) but did not affect the expression of IκB and p-IκB (p > 0.05) compared with the levels in the control CIA rats.


Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses
The effects of matrine on the expression of IκB, p-IκB, Cox-2, and iNOS in the synovial tissues of CIA rats. (a) Representative blots of IκB, p-IκB, Cox-2, and iNOS proteins; (b–f) Relative expression levels of IκB, p-IκB, IκB/p-IκB ration, Cox-2, and iNOS. Norm: normal rats; Cont: control rats; Dex: rats treated with dexamethasone. The data are presented as the mean ± SD (n = 4); * p < 0.05, ** p < 0.01, compared with the Cont.
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5037690&req=5

ijms-17-01410-f008: The effects of matrine on the expression of IκB, p-IκB, Cox-2, and iNOS in the synovial tissues of CIA rats. (a) Representative blots of IκB, p-IκB, Cox-2, and iNOS proteins; (b–f) Relative expression levels of IκB, p-IκB, IκB/p-IκB ration, Cox-2, and iNOS. Norm: normal rats; Cont: control rats; Dex: rats treated with dexamethasone. The data are presented as the mean ± SD (n = 4); * p < 0.05, ** p < 0.01, compared with the Cont.
Mentions: In the present investigation, we also evaluated the protein expression levels of IκB, p-IκB, Cox-2, and iNOS in synovial tissues of CIA rats using western blot assays. As shown in Figure 8, our results indicate that the expression level of IκB was significantly lower in control CIA rats (p < 0.01) than in normal rats. However, the expression levels of p-IκB, Cox-2, and iNOS were markedly up-regulated (p < 0.01) in these rats compared with those levels in normal rats. After treatment with dexamethasone (0.05 mg/kg/days), the expression levels of p-IκB, Cox-2, and iNOS were significantly down-regulated (p < 0.01) and the expression of IκB was elevated (p < 0.01) compared with levels in the control CIA rats. Furthermore, we also found that matrine (80 mg/kg) decreased the expression levels of p-IκB, Cox-2, and iNOS and increased the expression of IκB (p < 0.01) compared with the levels observed in the control CIA rats. Additionally, when administered at a dose of 40 mg/kg, matrine decreased the expression of p-IκB, Cox-2, and iNOS (p < 0.05) but had no effect on the expression of IκB (p > 0.05). Moreover, when administered at a dose of 20 mg/kg, matrine decreased the expression of Cox-2 and iNOS (p < 0.05) but did not affect the expression of IκB and p-IκB (p > 0.05) compared with the levels in the control CIA rats.

View Article: PubMed Central - PubMed

ABSTRACT

To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-&alpha;, IL-1&beta;, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-I&kappa;B, I&kappa;B, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-&alpha;, IL-1&beta;, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-I&kappa;B, Cox-2, and iNOS but up-regulated I&kappa;B in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-&kappa;B pathway.

No MeSH data available.


Related in: MedlinePlus