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Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses

View Article: PubMed Central - PubMed

ABSTRACT

To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-IκB, IκB, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-IκB, Cox-2, and iNOS but up-regulated IκB in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-κB pathway.

No MeSH data available.


Related in: MedlinePlus

The effects of matrine on the histopathological characteristics of joints and synovial tissues in CIA rats (×40). A–F: normal rats (A); control RA rats (B), arrows indicate cell infiltration (blue) or bone erosion (black); RA rats treated with dexamethasone (C); RA rats treated with matrine at 20 (D); 40 (E); and 80 (F) mg/kg/days.
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ijms-17-01410-f005: The effects of matrine on the histopathological characteristics of joints and synovial tissues in CIA rats (×40). A–F: normal rats (A); control RA rats (B), arrows indicate cell infiltration (blue) or bone erosion (black); RA rats treated with dexamethasone (C); RA rats treated with matrine at 20 (D); 40 (E); and 80 (F) mg/kg/days.

Mentions: The results of histopathological examinations of the CIA rat joints and synovial tissues are shown in Figure 5. Intact articular cartilage and normal joints and synovial tissues were observed in the normal rats. However, severe bone destruction and inflammation were observed in the H&E sections of the control CIA rats. The results of the histological analyses indicated that inflammation as well as joint and synovial tissue destruction were significantly reduced after treatment with dexamethasone and matrine.


Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses
The effects of matrine on the histopathological characteristics of joints and synovial tissues in CIA rats (×40). A–F: normal rats (A); control RA rats (B), arrows indicate cell infiltration (blue) or bone erosion (black); RA rats treated with dexamethasone (C); RA rats treated with matrine at 20 (D); 40 (E); and 80 (F) mg/kg/days.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037690&req=5

ijms-17-01410-f005: The effects of matrine on the histopathological characteristics of joints and synovial tissues in CIA rats (×40). A–F: normal rats (A); control RA rats (B), arrows indicate cell infiltration (blue) or bone erosion (black); RA rats treated with dexamethasone (C); RA rats treated with matrine at 20 (D); 40 (E); and 80 (F) mg/kg/days.
Mentions: The results of histopathological examinations of the CIA rat joints and synovial tissues are shown in Figure 5. Intact articular cartilage and normal joints and synovial tissues were observed in the normal rats. However, severe bone destruction and inflammation were observed in the H&E sections of the control CIA rats. The results of the histological analyses indicated that inflammation as well as joint and synovial tissue destruction were significantly reduced after treatment with dexamethasone and matrine.

View Article: PubMed Central - PubMed

ABSTRACT

To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-IκB, IκB, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-IκB, Cox-2, and iNOS but up-regulated IκB in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-κB pathway.

No MeSH data available.


Related in: MedlinePlus