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Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses

View Article: PubMed Central - PubMed

ABSTRACT

To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-IκB, IκB, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-IκB, Cox-2, and iNOS but up-regulated IκB in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-κB pathway.

No MeSH data available.


Related in: MedlinePlus

Effects of matrine on thymus and spleen indices in CIA rats. (a) Index of spleen; (b) Index of thymus. Norm: normal rats; Cont: control rats; Dex: rats treated with dexamethasone. The data are presented as the mean ± SD (n = 10); * p < 0.05, ** p < 0.01, compared with the Cont.
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ijms-17-01410-f004: Effects of matrine on thymus and spleen indices in CIA rats. (a) Index of spleen; (b) Index of thymus. Norm: normal rats; Cont: control rats; Dex: rats treated with dexamethasone. The data are presented as the mean ± SD (n = 10); * p < 0.05, ** p < 0.01, compared with the Cont.

Mentions: As shown in Figure 4, the thymus and spleen indices were significantly higher in CIA rats (p < 0.01) than in normal rats. After treatment with dexamethasone (0.05 mg/kg/d), both the thymus and the spleen indices were reduced (p < 0.01) compared with those of the control group. Similarly, treatment with matrine also reversed the increase in the thymus and spleen indices when administered at a dose of 80 mg/kg (p < 0.05, compared with the control group). In addition, when administered at a dose of 40 mg/kg, matrine significantly decreased the spleen index (p < 0.05, compared with the control group).


Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses
Effects of matrine on thymus and spleen indices in CIA rats. (a) Index of spleen; (b) Index of thymus. Norm: normal rats; Cont: control rats; Dex: rats treated with dexamethasone. The data are presented as the mean ± SD (n = 10); * p < 0.05, ** p < 0.01, compared with the Cont.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5037690&req=5

ijms-17-01410-f004: Effects of matrine on thymus and spleen indices in CIA rats. (a) Index of spleen; (b) Index of thymus. Norm: normal rats; Cont: control rats; Dex: rats treated with dexamethasone. The data are presented as the mean ± SD (n = 10); * p < 0.05, ** p < 0.01, compared with the Cont.
Mentions: As shown in Figure 4, the thymus and spleen indices were significantly higher in CIA rats (p < 0.01) than in normal rats. After treatment with dexamethasone (0.05 mg/kg/d), both the thymus and the spleen indices were reduced (p < 0.01) compared with those of the control group. Similarly, treatment with matrine also reversed the increase in the thymus and spleen indices when administered at a dose of 80 mg/kg (p < 0.05, compared with the control group). In addition, when administered at a dose of 40 mg/kg, matrine significantly decreased the spleen index (p < 0.05, compared with the control group).

View Article: PubMed Central - PubMed

ABSTRACT

To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-&alpha;, IL-1&beta;, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-I&kappa;B, I&kappa;B, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-&alpha;, IL-1&beta;, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-I&kappa;B, Cox-2, and iNOS but up-regulated I&kappa;B in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-&kappa;B pathway.

No MeSH data available.


Related in: MedlinePlus