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Saponin-Based Nanoemulsification Improves the Antioxidant Properties of Vitamin A and E in AML-12 Cells

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ABSTRACT

Our work aimed to investigate the protective effects of saponin-based nanoemulsions of vitamin A and E against oxidative stress-induced cellular damage in AML-12 cells. Saponin nanoemulsions of vitamin A (SAN) and vitamin E (SEN) were prepared by high-pressure homogenization and characterized in terms of size, zeta potential, and polydispersity index. SEN and SAN protect AML-12 cells against oxidative stress-induced cellular damage more efficiently via scavenging reactive oxygen species (ROS), and reducing DNA damage, protein carbonylation, and lipid peroxidation. These results provide valuable information for the development of nanoemulsion-based delivery systems that would improve the antioxidant properties of vitamin A and E.

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Nanoemulsion protection against H2O2-induced hepatocyte cytotoxicity. AML-12 cells were exposed to exogenous H2O2 for 16 h. Cell viability was determined by MTT assay. Vitamin and VE, vitamin E;nanoemulsion treatment protected hepatocytes against H2O2-induced cytotoxicity. C, control; SN, empty saponin nanoemulsion; VA, vitamin A; SAN, saponin nanoemulsion of vitamin A; VE, vitamin E; SEN, saponin nanoemulsion of vitamin E. All values are represented as mean ± SEM from three or more independent studies. * p < 0.05.
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ijms-17-01406-f001: Nanoemulsion protection against H2O2-induced hepatocyte cytotoxicity. AML-12 cells were exposed to exogenous H2O2 for 16 h. Cell viability was determined by MTT assay. Vitamin and VE, vitamin E;nanoemulsion treatment protected hepatocytes against H2O2-induced cytotoxicity. C, control; SN, empty saponin nanoemulsion; VA, vitamin A; SAN, saponin nanoemulsion of vitamin A; VE, vitamin E; SEN, saponin nanoemulsion of vitamin E. All values are represented as mean ± SEM from three or more independent studies. * p < 0.05.

Mentions: H2O2 is widely used as an oxidizing reagent to trigger oxidative stress. In our study, H2O2 was used to induce oxidative stress at various concentrations for 16 h, and cell viability was determined after 16 h of exposure. H2O2 exposure reduced cell viability in a dose-dependent manner (Figure S3b). To examine the protective effects of SAN and SEN, mouse hepatocytes were co-treated with nanoemulsions and 0.1 mM H2O2, and cell viability was measured. Results showed that SEN significantly protected (p < 0.05) against H2O2-induced cytotoxicity compared with vitamin E whereas A and SAN showed almost similar effect; however, SN alone did not provide a protective effect (Figure 1).


Saponin-Based Nanoemulsification Improves the Antioxidant Properties of Vitamin A and E in AML-12 Cells
Nanoemulsion protection against H2O2-induced hepatocyte cytotoxicity. AML-12 cells were exposed to exogenous H2O2 for 16 h. Cell viability was determined by MTT assay. Vitamin and VE, vitamin E;nanoemulsion treatment protected hepatocytes against H2O2-induced cytotoxicity. C, control; SN, empty saponin nanoemulsion; VA, vitamin A; SAN, saponin nanoemulsion of vitamin A; VE, vitamin E; SEN, saponin nanoemulsion of vitamin E. All values are represented as mean ± SEM from three or more independent studies. * p < 0.05.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5037686&req=5

ijms-17-01406-f001: Nanoemulsion protection against H2O2-induced hepatocyte cytotoxicity. AML-12 cells were exposed to exogenous H2O2 for 16 h. Cell viability was determined by MTT assay. Vitamin and VE, vitamin E;nanoemulsion treatment protected hepatocytes against H2O2-induced cytotoxicity. C, control; SN, empty saponin nanoemulsion; VA, vitamin A; SAN, saponin nanoemulsion of vitamin A; VE, vitamin E; SEN, saponin nanoemulsion of vitamin E. All values are represented as mean ± SEM from three or more independent studies. * p < 0.05.
Mentions: H2O2 is widely used as an oxidizing reagent to trigger oxidative stress. In our study, H2O2 was used to induce oxidative stress at various concentrations for 16 h, and cell viability was determined after 16 h of exposure. H2O2 exposure reduced cell viability in a dose-dependent manner (Figure S3b). To examine the protective effects of SAN and SEN, mouse hepatocytes were co-treated with nanoemulsions and 0.1 mM H2O2, and cell viability was measured. Results showed that SEN significantly protected (p < 0.05) against H2O2-induced cytotoxicity compared with vitamin E whereas A and SAN showed almost similar effect; however, SN alone did not provide a protective effect (Figure 1).

View Article: PubMed Central - PubMed

ABSTRACT

Our work aimed to investigate the protective effects of saponin-based nanoemulsions of vitamin A and E against oxidative stress-induced cellular damage in AML-12 cells. Saponin nanoemulsions of vitamin A (SAN) and vitamin E (SEN) were prepared by high-pressure homogenization and characterized in terms of size, zeta potential, and polydispersity index. SEN and SAN protect AML-12 cells against oxidative stress-induced cellular damage more efficiently via scavenging reactive oxygen species (ROS), and reducing DNA damage, protein carbonylation, and lipid peroxidation. These results provide valuable information for the development of nanoemulsion-based delivery systems that would improve the antioxidant properties of vitamin A and E.

No MeSH data available.


Related in: MedlinePlus