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Multifaceted Roles of ALG-2 in Ca 2+ -Regulated Membrane Trafficking

View Article: PubMed Central - PubMed

ABSTRACT

ALG-2 (gene name: PDCD6) is a penta-EF-hand Ca2+-binding protein and interacts with a variety of proteins in a Ca2+-dependent fashion. ALG-2 recognizes different types of identified motifs in Pro-rich regions by using different hydrophobic pockets, but other unknown modes of binding are also used for non-Pro-rich proteins. Most ALG-2-interacting proteins associate directly or indirectly with the plasma membrane or organelle membranes involving the endosomal sorting complex required for transport (ESCRT) system, coat protein complex II (COPII)-dependent ER-to-Golgi vesicular transport, and signal transduction from membrane receptors to downstream players. Binding of ALG-2 to targets may induce conformational change of the proteins. The ALG-2 dimer may also function as a Ca2+-dependent adaptor to bridge different partners and connect the subnetwork of interacting proteins.

No MeSH data available.


Related in: MedlinePlus

Topologically opposite budding of vesicles. Cytosolic proteins are recruited to the budding sites, but orientations of membrane deformation are opposite. (A) Endosomal sorting complex required for transport (ESCRT)-associated vesicle budding occurs from the cytosolic face of the membrane towards the extracellular space or into the organelle lumen; (B) Membrane coated with coat proteins (clathrin, coat protein complex I (COPI), and COPII) buds from the plasma membrane or organelle membranes into the cytosolic space.
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ijms-17-01401-f004: Topologically opposite budding of vesicles. Cytosolic proteins are recruited to the budding sites, but orientations of membrane deformation are opposite. (A) Endosomal sorting complex required for transport (ESCRT)-associated vesicle budding occurs from the cytosolic face of the membrane towards the extracellular space or into the organelle lumen; (B) Membrane coated with coat proteins (clathrin, coat protein complex I (COPI), and COPII) buds from the plasma membrane or organelle membranes into the cytosolic space.

Mentions: Membrane trafficking is mediated by vesicles that are carried from originating membranes to target membranes. Biogenesis of vesicles is initiated by deformation of the lipid bilayer in a restricted membrane area and then by budding and finally by abscission. Budding occurs in topologically opposite directions: (i) budding away from the cytosol; and (ii) budding into the cytosol (Figure 4). ALG-2 participates in part and plays regulatory roles by Ca2+-dependently interacting with key players in both systems.


Multifaceted Roles of ALG-2 in Ca 2+ -Regulated Membrane Trafficking
Topologically opposite budding of vesicles. Cytosolic proteins are recruited to the budding sites, but orientations of membrane deformation are opposite. (A) Endosomal sorting complex required for transport (ESCRT)-associated vesicle budding occurs from the cytosolic face of the membrane towards the extracellular space or into the organelle lumen; (B) Membrane coated with coat proteins (clathrin, coat protein complex I (COPI), and COPII) buds from the plasma membrane or organelle membranes into the cytosolic space.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5037681&req=5

ijms-17-01401-f004: Topologically opposite budding of vesicles. Cytosolic proteins are recruited to the budding sites, but orientations of membrane deformation are opposite. (A) Endosomal sorting complex required for transport (ESCRT)-associated vesicle budding occurs from the cytosolic face of the membrane towards the extracellular space or into the organelle lumen; (B) Membrane coated with coat proteins (clathrin, coat protein complex I (COPI), and COPII) buds from the plasma membrane or organelle membranes into the cytosolic space.
Mentions: Membrane trafficking is mediated by vesicles that are carried from originating membranes to target membranes. Biogenesis of vesicles is initiated by deformation of the lipid bilayer in a restricted membrane area and then by budding and finally by abscission. Budding occurs in topologically opposite directions: (i) budding away from the cytosol; and (ii) budding into the cytosol (Figure 4). ALG-2 participates in part and plays regulatory roles by Ca2+-dependently interacting with key players in both systems.

View Article: PubMed Central - PubMed

ABSTRACT

ALG-2 (gene name: PDCD6) is a penta-EF-hand Ca2+-binding protein and interacts with a variety of proteins in a Ca2+-dependent fashion. ALG-2 recognizes different types of identified motifs in Pro-rich regions by using different hydrophobic pockets, but other unknown modes of binding are also used for non-Pro-rich proteins. Most ALG-2-interacting proteins associate directly or indirectly with the plasma membrane or organelle membranes involving the endosomal sorting complex required for transport (ESCRT) system, coat protein complex II (COPII)-dependent ER-to-Golgi vesicular transport, and signal transduction from membrane receptors to downstream players. Binding of ALG-2 to targets may induce conformational change of the proteins. The ALG-2 dimer may also function as a Ca2+-dependent adaptor to bridge different partners and connect the subnetwork of interacting proteins.

No MeSH data available.


Related in: MedlinePlus