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Multifaceted Roles of ALG-2 in Ca 2+ -Regulated Membrane Trafficking

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ABSTRACT

ALG-2 (gene name: PDCD6) is a penta-EF-hand Ca2+-binding protein and interacts with a variety of proteins in a Ca2+-dependent fashion. ALG-2 recognizes different types of identified motifs in Pro-rich regions by using different hydrophobic pockets, but other unknown modes of binding are also used for non-Pro-rich proteins. Most ALG-2-interacting proteins associate directly or indirectly with the plasma membrane or organelle membranes involving the endosomal sorting complex required for transport (ESCRT) system, coat protein complex II (COPII)-dependent ER-to-Golgi vesicular transport, and signal transduction from membrane receptors to downstream players. Binding of ALG-2 to targets may induce conformational change of the proteins. The ALG-2 dimer may also function as a Ca2+-dependent adaptor to bridge different partners and connect the subnetwork of interacting proteins.

No MeSH data available.


Three types of Pro-rich ALG-2-binding motifs. Amino acid sequences of human Pro-rich proteins containing ALG-2-binding motifs (Type 1, Type 2, and Type 3) are presented. X, any amino acid; Φ, hydrophobic; Ω, large side chain. Amino acids compatible with ALG-2-binding motifs are indicated in red except for X positions. IST1, increased sodium tolerance-1; PLSCR3, phospholipid scramblase 3; PATL1, protein associated with topoisomerase II (PAT)-like 1; CHERP; calcium homeostasis and endoplasmic reticulum protein.
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ijms-17-01401-f003: Three types of Pro-rich ALG-2-binding motifs. Amino acid sequences of human Pro-rich proteins containing ALG-2-binding motifs (Type 1, Type 2, and Type 3) are presented. X, any amino acid; Φ, hydrophobic; Ω, large side chain. Amino acids compatible with ALG-2-binding motifs are indicated in red except for X positions. IST1, increased sodium tolerance-1; PLSCR3, phospholipid scramblase 3; PATL1, protein associated with topoisomerase II (PAT)-like 1; CHERP; calcium homeostasis and endoplasmic reticulum protein.

Mentions: Determination of ALG-2-binding sites in the interacting proteins and amino acid substitution experiments have revealed three types of Pro-based ALG-2-binding motifs (ABMs) (Figure 3) and one non-Pro-based ABM.


Multifaceted Roles of ALG-2 in Ca 2+ -Regulated Membrane Trafficking
Three types of Pro-rich ALG-2-binding motifs. Amino acid sequences of human Pro-rich proteins containing ALG-2-binding motifs (Type 1, Type 2, and Type 3) are presented. X, any amino acid; Φ, hydrophobic; Ω, large side chain. Amino acids compatible with ALG-2-binding motifs are indicated in red except for X positions. IST1, increased sodium tolerance-1; PLSCR3, phospholipid scramblase 3; PATL1, protein associated with topoisomerase II (PAT)-like 1; CHERP; calcium homeostasis and endoplasmic reticulum protein.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5037681&req=5

ijms-17-01401-f003: Three types of Pro-rich ALG-2-binding motifs. Amino acid sequences of human Pro-rich proteins containing ALG-2-binding motifs (Type 1, Type 2, and Type 3) are presented. X, any amino acid; Φ, hydrophobic; Ω, large side chain. Amino acids compatible with ALG-2-binding motifs are indicated in red except for X positions. IST1, increased sodium tolerance-1; PLSCR3, phospholipid scramblase 3; PATL1, protein associated with topoisomerase II (PAT)-like 1; CHERP; calcium homeostasis and endoplasmic reticulum protein.
Mentions: Determination of ALG-2-binding sites in the interacting proteins and amino acid substitution experiments have revealed three types of Pro-based ALG-2-binding motifs (ABMs) (Figure 3) and one non-Pro-based ABM.

View Article: PubMed Central - PubMed

ABSTRACT

ALG-2 (gene name: PDCD6) is a penta-EF-hand Ca2+-binding protein and interacts with a variety of proteins in a Ca2+-dependent fashion. ALG-2 recognizes different types of identified motifs in Pro-rich regions by using different hydrophobic pockets, but other unknown modes of binding are also used for non-Pro-rich proteins. Most ALG-2-interacting proteins associate directly or indirectly with the plasma membrane or organelle membranes involving the endosomal sorting complex required for transport (ESCRT) system, coat protein complex II (COPII)-dependent ER-to-Golgi vesicular transport, and signal transduction from membrane receptors to downstream players. Binding of ALG-2 to targets may induce conformational change of the proteins. The ALG-2 dimer may also function as a Ca2+-dependent adaptor to bridge different partners and connect the subnetwork of interacting proteins.

No MeSH data available.