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Calcium-Sensing Receptor in Human Peripheral Blood T Lymphocytes Is Involved in the AMI Onset and Progression through the NF- κ B Signaling Pathway

View Article: PubMed Central - PubMed

ABSTRACT

Acute myocardial infarction (AMI) is a condition triggered by an inflammatory process that seriously affects human health. Calcium-sensing receptor (CaSR) in T lymphocytes is involved during the inflammation reaction. However, the relationship between them is not very clear. In this study, we collected human peripheral blood T lymphocytes from patients with AMI and in different stages of percutaneous coronary intervention (PCI) (at the onset of AMI, the first day after PCI (PCI-1), PCI-3, and PCI-5) to study the CaSR and NF-κB pathway protein expression, cytokine release and T cell apoptosis. The results showed that the expressions of CaSR, P-p65, Caspase-12, and the secretions of Th-1 and Th-2 type cytokines were increased at the onset of AMI, especially on the PCI-1. Meanwhile, the apoptosis rate of CD3+, CD4+ and CD8+ T lymphocytes also increased. However, from PCI-3, all the indicators began to decline. In addition, we also found that positive CaSR small interfering RNA (siRNA) transfection in T lymphocytes and NF-κB pathway blocker Bay-11-7082 reversed the increased expressions of CaSR, P-p65, Caspase-12, reduced the secretions of Th-1 and Th-2 type cytokines, and decreased T lymphocytes apoptosis rate not only in the AMI patients but also in the normal controls. All of these results indicated that CaSR in the human peripheral blood T lymphocytes were involved in the AMI onset and progression, which probably was related to the NF-κB pathway. Our study demonstrated the relationship between AMI and CaSR, and will provide new effective prevention theory and new targets for drug treatment.

No MeSH data available.


Related in: MedlinePlus

Apoptosis rate of T lymphocytes. FACS results showed that the apoptosis rate of all the T cells of CD3+ labeled with APC (A); CD3+CD4+ labeled with PE (B); and CD3+CD8+ labeled with APC-Cy7 (C) increased at the onset of AMI and PCI-1, especially the CD3+ lymphocytes. However, they all returned to normal levels at PCI-3. The apoptosis rate of all these T cells was quantified by densitometry (n = 20) (D). * p < 0.05 vs. Normal group; #p < 0.05 vs. AMI group; ☆p < 0.05 vs. PCI-1 group.
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ijms-17-01397-f002: Apoptosis rate of T lymphocytes. FACS results showed that the apoptosis rate of all the T cells of CD3+ labeled with APC (A); CD3+CD4+ labeled with PE (B); and CD3+CD8+ labeled with APC-Cy7 (C) increased at the onset of AMI and PCI-1, especially the CD3+ lymphocytes. However, they all returned to normal levels at PCI-3. The apoptosis rate of all these T cells was quantified by densitometry (n = 20) (D). * p < 0.05 vs. Normal group; #p < 0.05 vs. AMI group; ☆p < 0.05 vs. PCI-1 group.

Mentions: Using the Fluorescence activated cell sorting (FACS), we observed the T-cell apoptosis condition. Our results indicated that the apoptosis ratio of CD3+ T lymphocytes was about 1.5-fold in AMI, and 3-fold in PCI-1 as much as the control group was (Figure 2A) (p < 0.05); the apoptosis ratio of CD4+ T lymphocytes was about 2.2-fold in AMI and 3.3-fold in PCI-1, as much as the control group (Figure 2B) (p < 0.05); and the apoptosis ratio of CD8+ T lymphocytes increased to 2-fold in AMI and to 3.4-fold in PCI-1 more than the control group (p < 0.05). They all returned to normal in PCI-3 (Figure 2C).


Calcium-Sensing Receptor in Human Peripheral Blood T Lymphocytes Is Involved in the AMI Onset and Progression through the NF- κ B Signaling Pathway
Apoptosis rate of T lymphocytes. FACS results showed that the apoptosis rate of all the T cells of CD3+ labeled with APC (A); CD3+CD4+ labeled with PE (B); and CD3+CD8+ labeled with APC-Cy7 (C) increased at the onset of AMI and PCI-1, especially the CD3+ lymphocytes. However, they all returned to normal levels at PCI-3. The apoptosis rate of all these T cells was quantified by densitometry (n = 20) (D). * p < 0.05 vs. Normal group; #p < 0.05 vs. AMI group; ☆p < 0.05 vs. PCI-1 group.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5037677&req=5

ijms-17-01397-f002: Apoptosis rate of T lymphocytes. FACS results showed that the apoptosis rate of all the T cells of CD3+ labeled with APC (A); CD3+CD4+ labeled with PE (B); and CD3+CD8+ labeled with APC-Cy7 (C) increased at the onset of AMI and PCI-1, especially the CD3+ lymphocytes. However, they all returned to normal levels at PCI-3. The apoptosis rate of all these T cells was quantified by densitometry (n = 20) (D). * p < 0.05 vs. Normal group; #p < 0.05 vs. AMI group; ☆p < 0.05 vs. PCI-1 group.
Mentions: Using the Fluorescence activated cell sorting (FACS), we observed the T-cell apoptosis condition. Our results indicated that the apoptosis ratio of CD3+ T lymphocytes was about 1.5-fold in AMI, and 3-fold in PCI-1 as much as the control group was (Figure 2A) (p < 0.05); the apoptosis ratio of CD4+ T lymphocytes was about 2.2-fold in AMI and 3.3-fold in PCI-1, as much as the control group (Figure 2B) (p < 0.05); and the apoptosis ratio of CD8+ T lymphocytes increased to 2-fold in AMI and to 3.4-fold in PCI-1 more than the control group (p < 0.05). They all returned to normal in PCI-3 (Figure 2C).

View Article: PubMed Central - PubMed

ABSTRACT

Acute myocardial infarction (AMI) is a condition triggered by an inflammatory process that seriously affects human health. Calcium-sensing receptor (CaSR) in T lymphocytes is involved during the inflammation reaction. However, the relationship between them is not very clear. In this study, we collected human peripheral blood T lymphocytes from patients with AMI and in different stages of percutaneous coronary intervention (PCI) (at the onset of AMI, the first day after PCI (PCI-1), PCI-3, and PCI-5) to study the CaSR and NF-&kappa;B pathway protein expression, cytokine release and T cell apoptosis. The results showed that the expressions of CaSR, P-p65, Caspase-12, and the secretions of Th-1 and Th-2 type cytokines were increased at the onset of AMI, especially on the PCI-1. Meanwhile, the apoptosis rate of CD3+, CD4+ and CD8+ T lymphocytes also increased. However, from PCI-3, all the indicators began to decline. In addition, we also found that positive CaSR small interfering RNA (siRNA) transfection in T lymphocytes and NF-&kappa;B pathway blocker Bay-11-7082 reversed the increased expressions of CaSR, P-p65, Caspase-12, reduced the secretions of Th-1 and Th-2 type cytokines, and decreased T lymphocytes apoptosis rate not only in the AMI patients but also in the normal controls. All of these results indicated that CaSR in the human peripheral blood T lymphocytes were involved in the AMI onset and progression, which probably was related to the NF-&kappa;B pathway. Our study demonstrated the relationship between AMI and CaSR, and will provide new effective prevention theory and new targets for drug treatment.

No MeSH data available.


Related in: MedlinePlus