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Calcium-Sensing Receptor in Human Peripheral Blood T Lymphocytes Is Involved in the AMI Onset and Progression through the NF- κ B Signaling Pathway

View Article: PubMed Central - PubMed

ABSTRACT

Acute myocardial infarction (AMI) is a condition triggered by an inflammatory process that seriously affects human health. Calcium-sensing receptor (CaSR) in T lymphocytes is involved during the inflammation reaction. However, the relationship between them is not very clear. In this study, we collected human peripheral blood T lymphocytes from patients with AMI and in different stages of percutaneous coronary intervention (PCI) (at the onset of AMI, the first day after PCI (PCI-1), PCI-3, and PCI-5) to study the CaSR and NF-κB pathway protein expression, cytokine release and T cell apoptosis. The results showed that the expressions of CaSR, P-p65, Caspase-12, and the secretions of Th-1 and Th-2 type cytokines were increased at the onset of AMI, especially on the PCI-1. Meanwhile, the apoptosis rate of CD3+, CD4+ and CD8+ T lymphocytes also increased. However, from PCI-3, all the indicators began to decline. In addition, we also found that positive CaSR small interfering RNA (siRNA) transfection in T lymphocytes and NF-κB pathway blocker Bay-11-7082 reversed the increased expressions of CaSR, P-p65, Caspase-12, reduced the secretions of Th-1 and Th-2 type cytokines, and decreased T lymphocytes apoptosis rate not only in the AMI patients but also in the normal controls. All of these results indicated that CaSR in the human peripheral blood T lymphocytes were involved in the AMI onset and progression, which probably was related to the NF-κB pathway. Our study demonstrated the relationship between AMI and CaSR, and will provide new effective prevention theory and new targets for drug treatment.

No MeSH data available.


Related in: MedlinePlus

Protein and mRNA expressions of calcium-sensing receptor (CaSR) (A,B) and Caspase12 (D,E) in T lymphocytes and the phosphorylation level of NF-κB pathway protein (C) during the different stages in acute myocardial infarction (AMI). Protein expressions in T lymphocytes were tested by Western blot and qRT-PCR quantitative analysis (n = 20). Expression protein was quantified by densitometry. Expression results are representative of five experiments. * p < 0.05 vs. normal group; #p < 0.05 vs. AMI group; ☆p < 0.05 vs. PCI-1 group; □p < 0.05 vs. PCI-3 group.
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ijms-17-01397-f001: Protein and mRNA expressions of calcium-sensing receptor (CaSR) (A,B) and Caspase12 (D,E) in T lymphocytes and the phosphorylation level of NF-κB pathway protein (C) during the different stages in acute myocardial infarction (AMI). Protein expressions in T lymphocytes were tested by Western blot and qRT-PCR quantitative analysis (n = 20). Expression protein was quantified by densitometry. Expression results are representative of five experiments. * p < 0.05 vs. normal group; #p < 0.05 vs. AMI group; ☆p < 0.05 vs. PCI-1 group; □p < 0.05 vs. PCI-3 group.

Mentions: Western blotting detected two relative molecular mass of 120–170 KDa and 90–110 KDa of the CaSR protein. qRT-PCR quantitative analysis was used to detected the CaSR mRNA expression. From the results, it can find that both the CaSR protein and mRNA in T cells increased at the AMI onset and PCI-1 compared normal group, especially in the PCI-1 (p < 0.05), then began to descend from PCI-3. The CaSR mRNA level descended more quickly than CaSR protein. (Figure 1A,B).


Calcium-Sensing Receptor in Human Peripheral Blood T Lymphocytes Is Involved in the AMI Onset and Progression through the NF- κ B Signaling Pathway
Protein and mRNA expressions of calcium-sensing receptor (CaSR) (A,B) and Caspase12 (D,E) in T lymphocytes and the phosphorylation level of NF-κB pathway protein (C) during the different stages in acute myocardial infarction (AMI). Protein expressions in T lymphocytes were tested by Western blot and qRT-PCR quantitative analysis (n = 20). Expression protein was quantified by densitometry. Expression results are representative of five experiments. * p < 0.05 vs. normal group; #p < 0.05 vs. AMI group; ☆p < 0.05 vs. PCI-1 group; □p < 0.05 vs. PCI-3 group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037677&req=5

ijms-17-01397-f001: Protein and mRNA expressions of calcium-sensing receptor (CaSR) (A,B) and Caspase12 (D,E) in T lymphocytes and the phosphorylation level of NF-κB pathway protein (C) during the different stages in acute myocardial infarction (AMI). Protein expressions in T lymphocytes were tested by Western blot and qRT-PCR quantitative analysis (n = 20). Expression protein was quantified by densitometry. Expression results are representative of five experiments. * p < 0.05 vs. normal group; #p < 0.05 vs. AMI group; ☆p < 0.05 vs. PCI-1 group; □p < 0.05 vs. PCI-3 group.
Mentions: Western blotting detected two relative molecular mass of 120–170 KDa and 90–110 KDa of the CaSR protein. qRT-PCR quantitative analysis was used to detected the CaSR mRNA expression. From the results, it can find that both the CaSR protein and mRNA in T cells increased at the AMI onset and PCI-1 compared normal group, especially in the PCI-1 (p < 0.05), then began to descend from PCI-3. The CaSR mRNA level descended more quickly than CaSR protein. (Figure 1A,B).

View Article: PubMed Central - PubMed

ABSTRACT

Acute myocardial infarction (AMI) is a condition triggered by an inflammatory process that seriously affects human health. Calcium-sensing receptor (CaSR) in T lymphocytes is involved during the inflammation reaction. However, the relationship between them is not very clear. In this study, we collected human peripheral blood T lymphocytes from patients with AMI and in different stages of percutaneous coronary intervention (PCI) (at the onset of AMI, the first day after PCI (PCI-1), PCI-3, and PCI-5) to study the CaSR and NF-&kappa;B pathway protein expression, cytokine release and T cell apoptosis. The results showed that the expressions of CaSR, P-p65, Caspase-12, and the secretions of Th-1 and Th-2 type cytokines were increased at the onset of AMI, especially on the PCI-1. Meanwhile, the apoptosis rate of CD3+, CD4+ and CD8+ T lymphocytes also increased. However, from PCI-3, all the indicators began to decline. In addition, we also found that positive CaSR small interfering RNA (siRNA) transfection in T lymphocytes and NF-&kappa;B pathway blocker Bay-11-7082 reversed the increased expressions of CaSR, P-p65, Caspase-12, reduced the secretions of Th-1 and Th-2 type cytokines, and decreased T lymphocytes apoptosis rate not only in the AMI patients but also in the normal controls. All of these results indicated that CaSR in the human peripheral blood T lymphocytes were involved in the AMI onset and progression, which probably was related to the NF-&kappa;B pathway. Our study demonstrated the relationship between AMI and CaSR, and will provide new effective prevention theory and new targets for drug treatment.

No MeSH data available.


Related in: MedlinePlus