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Autologous bone grafts with MSCs or FGF-2 accelerate bone union in large bone defects

View Article: PubMed Central - PubMed

ABSTRACT

Bacground: Although the contribution of fibroblast growth factor (FGF)-2 and mesenchymal stromal cells (MSCs) to bone formation is well known, few studies have investigated the combination of an autologous bone graft with FGF-2 or MSCs for large bone defects.

Methods: We studied an atrophic non-union model with a large bone defect, created by resecting a 10-mm section from the center of each femoral shaft of 12-week-old Sprague-Dawley rats. The periosteum of the proximal and distal ends of the femur was cauterized circumferentially, and excised portions were used in the contralateral femur as autologous bone grafts. The rats were randomized to three groups and given no further treatment (group A), administered FGF-2 at 20 μg/20 μL (group B), or 1.0 × 106 MSCs (group C). Radiographs were taken every 2 weeks up to 12 weeks, with CT performed at 12 weeks. Harvested femurs were stained with toluidine blue and evaluated using radiographic and histology scores.

Results: Radiographic and histological evaluation showed that bone union had been achieved at 12 weeks in group C, while group B showed callus formation and bridging callus but non-union, and in group A, callus formation alone was evident. Both radiographic and histological scores were significantly higher at 2, 4, 6, 8, 10, and 12 weeks in groups B and C than group A and also significantly higher in group C than group B at 12 weeks.

Conclusions: These data suggest that autologous bone grafts in combination with MSCs benefit difficult cases which cannot be treated with autologous bone grafts alone.

No MeSH data available.


CT images in each group at 12 weeks. In CT images, there was abundant callus formation around the junction of femur and bone graft in all groups; however, there was no bridging callus formation in group A or B. In contrast, complete bone union could be observed in group C
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Fig4: CT images in each group at 12 weeks. In CT images, there was abundant callus formation around the junction of femur and bone graft in all groups; however, there was no bridging callus formation in group A or B. In contrast, complete bone union could be observed in group C

Mentions: In the radiographs at 2 weeks, no callus formation was evident in any group. However, there was obvious callus formation on the proximal and distal sides of the femur and on both sides of the autologous bone graft in groups B and C at 4 weeks while callus formation was only present on the proximal and distal sides of the femur with no callus on the autologous bone graft in group A. Bridging callus formation could be observed at 6 weeks in group C and was also observed at 10 weeks in group B. In group A, there was no bridging callus formation at 12 weeks. The bridging callus in group C was remodeled, and bone union was observed at 12 weeks (Fig. 2). The radiographic scores in groups B and C were significantly higher than that in group A and there was no significant difference between groups B and C at 2, 4, 6, 8, or 10 weeks. At 12 weeks, the radiographic score in group C was significantly higher than that in group B (Table 1) (Fig. 3). In CT images, there was abundant callus formation around the junction of femur and bone graft in all groups; however, there was no bridging callus formation in group A or B. In contrast, complete bone union could be observed in group C (Fig. 4).Fig. 2


Autologous bone grafts with MSCs or FGF-2 accelerate bone union in large bone defects
CT images in each group at 12 weeks. In CT images, there was abundant callus formation around the junction of femur and bone graft in all groups; however, there was no bridging callus formation in group A or B. In contrast, complete bone union could be observed in group C
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5037630&req=5

Fig4: CT images in each group at 12 weeks. In CT images, there was abundant callus formation around the junction of femur and bone graft in all groups; however, there was no bridging callus formation in group A or B. In contrast, complete bone union could be observed in group C
Mentions: In the radiographs at 2 weeks, no callus formation was evident in any group. However, there was obvious callus formation on the proximal and distal sides of the femur and on both sides of the autologous bone graft in groups B and C at 4 weeks while callus formation was only present on the proximal and distal sides of the femur with no callus on the autologous bone graft in group A. Bridging callus formation could be observed at 6 weeks in group C and was also observed at 10 weeks in group B. In group A, there was no bridging callus formation at 12 weeks. The bridging callus in group C was remodeled, and bone union was observed at 12 weeks (Fig. 2). The radiographic scores in groups B and C were significantly higher than that in group A and there was no significant difference between groups B and C at 2, 4, 6, 8, or 10 weeks. At 12 weeks, the radiographic score in group C was significantly higher than that in group B (Table 1) (Fig. 3). In CT images, there was abundant callus formation around the junction of femur and bone graft in all groups; however, there was no bridging callus formation in group A or B. In contrast, complete bone union could be observed in group C (Fig. 4).Fig. 2

View Article: PubMed Central - PubMed

ABSTRACT

Bacground: Although the contribution of fibroblast growth factor (FGF)-2 and mesenchymal stromal cells (MSCs) to bone formation is well known, few studies have investigated the combination of an autologous bone graft with FGF-2 or MSCs for large bone defects.

Methods: We studied an atrophic non-union model with a large bone defect, created by resecting a 10-mm section from the center of each femoral shaft of 12-week-old Sprague-Dawley rats. The periosteum of the proximal and distal ends of the femur was cauterized circumferentially, and excised portions were used in the contralateral femur as autologous bone grafts. The rats were randomized to three groups and given no further treatment (group A), administered FGF-2 at 20 μg/20 μL (group B), or 1.0 × 106 MSCs (group C). Radiographs were taken every 2 weeks up to 12 weeks, with CT performed at 12 weeks. Harvested femurs were stained with toluidine blue and evaluated using radiographic and histology scores.

Results: Radiographic and histological evaluation showed that bone union had been achieved at 12 weeks in group C, while group B showed callus formation and bridging callus but non-union, and in group A, callus formation alone was evident. Both radiographic and histological scores were significantly higher at 2, 4, 6, 8, 10, and 12 weeks in groups B and C than group A and also significantly higher in group C than group B at 12 weeks.

Conclusions: These data suggest that autologous bone grafts in combination with MSCs benefit difficult cases which cannot be treated with autologous bone grafts alone.

No MeSH data available.