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Complete genome sequence of Pseudomonas citronellolis P3B5, a candidate for microbial phyllo-remediation of hydrocarbon-contaminated sites

View Article: PubMed Central - PubMed

ABSTRACT

Pseudomonas citronellolis is a Gram negative, motile gammaproteobacterium belonging to the order Pseudomonadales and the family Pseudomonadaceae. We isolated strain P3B5 from the phyllosphere of basil plants (Ocimum basilicum L.). Here we describe the physiology of this microorganism, its full genome sequence, and detailed annotation. The 6.95 Mbp genome contains 6071 predicted protein coding sequences and 96 RNA coding sequences. P. citronellolis has been the subject of many studies including the investigation of long-chain aliphatic compounds and terpene degradation. Plant leaves are covered by long-chain aliphates making up a waxy layer that is associated with the leaf cuticle. In addition, basil leaves are known to contain high amounts of terpenoid substances, hinting to a potential nutrient niche that might be exploited by P. citronellolis. Furthermore, the isolated strain exhibited resistance to several antibiotics. To evaluate the potential of this strain as source of transferable antibiotic resistance genes on raw consumed herbs we therefore investigated if those resistances are encoded on mobile genetic elements. The availability of the genome will be helpful for comparative genomics of the phylogenetically broad pseudomonads, in particular with the sequence of the P. citronellolis type strain PRJDB205 not yet publicly available. The genome is discussed with respect to a phyllosphere related lifestyle, aliphate and terpenoid degradation, and antibiotic resistance.

Electronic supplementary material: The online version of this article (doi:10.1186/s40793-016-0190-6) contains supplementary material, which is available to authorized users.

No MeSH data available.


a Scanning-electron micrograph of P. citronellolis P3B5. bP. citronellolis P3B5 grown on LB agar for 4 days. cP. citronellolis P3B5 grown in M9 minimal medium for 20 h excited by UV light exhibiting strong fluorescence. d Growth of P. citronellolis P3B5 was analyzed by measuring the optical density at 600 nm at the different temperatures for 24 h. 12 to 15 replicate measurements were performed for each temperature. By plotting the observed growth rate during the exponential growth phase at different temperatures, it was determined that the ideal growth temperature of P. citronellolis P3B5 is around 37 °C. No growth was observed at 4 °C
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Fig1: a Scanning-electron micrograph of P. citronellolis P3B5. bP. citronellolis P3B5 grown on LB agar for 4 days. cP. citronellolis P3B5 grown in M9 minimal medium for 20 h excited by UV light exhibiting strong fluorescence. d Growth of P. citronellolis P3B5 was analyzed by measuring the optical density at 600 nm at the different temperatures for 24 h. 12 to 15 replicate measurements were performed for each temperature. By plotting the observed growth rate during the exponential growth phase at different temperatures, it was determined that the ideal growth temperature of P. citronellolis P3B5 is around 37 °C. No growth was observed at 4 °C

Mentions: The species P. citronellolis was proposed and described by Seubert [7] and isolated from soil collected under pine trees in northern Virginia, USA. P. citronellolis is a Gram negative, rod-shaped, gammaproteobacterium that is approximately 2 μm in length and 0.5 μm in width (Fig. 1a), motile by one polar flagellum, and non-spore-forming [7]. On Lysogeny Broth agar P. citronellolis forms white, round colonies (Fig. 1b), that produce green fluorescent pigments after several days of incubation. Grown in liquid M9 minimal medium the production of green fluorescent pigments is even more pronounced (Fig. 1c).Fig. 1


Complete genome sequence of Pseudomonas citronellolis P3B5, a candidate for microbial phyllo-remediation of hydrocarbon-contaminated sites
a Scanning-electron micrograph of P. citronellolis P3B5. bP. citronellolis P3B5 grown on LB agar for 4 days. cP. citronellolis P3B5 grown in M9 minimal medium for 20 h excited by UV light exhibiting strong fluorescence. d Growth of P. citronellolis P3B5 was analyzed by measuring the optical density at 600 nm at the different temperatures for 24 h. 12 to 15 replicate measurements were performed for each temperature. By plotting the observed growth rate during the exponential growth phase at different temperatures, it was determined that the ideal growth temperature of P. citronellolis P3B5 is around 37 °C. No growth was observed at 4 °C
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5037603&req=5

Fig1: a Scanning-electron micrograph of P. citronellolis P3B5. bP. citronellolis P3B5 grown on LB agar for 4 days. cP. citronellolis P3B5 grown in M9 minimal medium for 20 h excited by UV light exhibiting strong fluorescence. d Growth of P. citronellolis P3B5 was analyzed by measuring the optical density at 600 nm at the different temperatures for 24 h. 12 to 15 replicate measurements were performed for each temperature. By plotting the observed growth rate during the exponential growth phase at different temperatures, it was determined that the ideal growth temperature of P. citronellolis P3B5 is around 37 °C. No growth was observed at 4 °C
Mentions: The species P. citronellolis was proposed and described by Seubert [7] and isolated from soil collected under pine trees in northern Virginia, USA. P. citronellolis is a Gram negative, rod-shaped, gammaproteobacterium that is approximately 2 μm in length and 0.5 μm in width (Fig. 1a), motile by one polar flagellum, and non-spore-forming [7]. On Lysogeny Broth agar P. citronellolis forms white, round colonies (Fig. 1b), that produce green fluorescent pigments after several days of incubation. Grown in liquid M9 minimal medium the production of green fluorescent pigments is even more pronounced (Fig. 1c).Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

Pseudomonas citronellolis is a Gram negative, motile gammaproteobacterium belonging to the order Pseudomonadales and the family Pseudomonadaceae. We isolated strain P3B5 from the phyllosphere of basil plants (Ocimum basilicum L.). Here we describe the physiology of this microorganism, its full genome sequence, and detailed annotation. The 6.95 Mbp genome contains 6071 predicted protein coding sequences and 96 RNA coding sequences. P. citronellolis has been the subject of many studies including the investigation of long-chain aliphatic compounds and terpene degradation. Plant leaves are covered by long-chain aliphates making up a waxy layer that is associated with the leaf cuticle. In addition, basil leaves are known to contain high amounts of terpenoid substances, hinting to a potential nutrient niche that might be exploited by P. citronellolis. Furthermore, the isolated strain exhibited resistance to several antibiotics. To evaluate the potential of this strain as source of transferable antibiotic resistance genes on raw consumed herbs we therefore investigated if those resistances are encoded on mobile genetic elements. The availability of the genome will be helpful for comparative genomics of the phylogenetically broad pseudomonads, in particular with the sequence of the P. citronellolis type strain PRJDB205 not yet publicly available. The genome is discussed with respect to a phyllosphere related lifestyle, aliphate and terpenoid degradation, and antibiotic resistance.

Electronic supplementary material: The online version of this article (doi:10.1186/s40793-016-0190-6) contains supplementary material, which is available to authorized users.

No MeSH data available.