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Dendrophthoe pentandra (L.) Miq extract effectively inhibits inflammation, proliferation and induces p53 expression on colitis-associated colon cancer

View Article: PubMed Central - PubMed

ABSTRACT

Background: Indonesian mistletoe grows on various trees. Mango Mistletoes (Dendrophthoe pentandra) is one type of mistletoe that grown on mango tree (.benalu mangga in bahasa Indonesia). Our study used mistletoe as a parasitic plant that has been used for traditional medicine. It has been known that Dendrophtoe pentandra extract (DPE) anti-inflammatory and anticancer. Furthermore, it is necessary to follow-up study in vivo to evaluate the response to treatment of new cancer therapeutic agents. This research aimed to determine the levels of IL-22, myeloperoxide (MPO), proliferation and wild-type p53 expression after the administration of DPE to murine models of CAC.

Methods: Mouse colitis associated colon cancer (CAC) was induced firstly by azoxymethane (AOM) and followed by administration of drinking water containing 5 % dextran sodium sulfate (DSS) in a cycle protocol, each cycle consisted of seven days of 5 % DSS in the drinking water and followed by seven days of regular water. This study consists of five treatment groups: I was treated water only (control), II was administrated by (DSS only, without DPE), (III-V) were administrated by DPE (125 mg/kg BW, 250 mg/kg BW and 500 mg/kg BW) respectively. The administrated of DPE were started from the 8th weeks, were continued until 21 weeks. At the end of 21 weeks of the experiment, mice were sacrificed, colon tissue was removed, and then subjected to ELISA, flow cytometry, real-time PCR and histology examination.

Results: Administration of DPE 250 mg/kgBW significantly reduce the levels of IL-22 and MPO compared with DSS only group (p < 0.001; p < 0.001). Colonic epithelial cells proliferation of group IV (DPE 250 mg/kgBW) were significantly lower than III and V groups. There was no significant change in the S phase in mice were treated DPE 125 mg/kg BW and 500 mg/kg BW, while administration of DPE 250 mg/kg BW was able to increase the percentage of cells in S phase. The expression of mRNA p53 was up regulated in mice received DPE 125 mg/kg BW.

Conclusion: These findings indicate that the DPE could inhibit colonic epithelial cells proliferation through p53 pathway independently. This study also showed that DPE could be potential sources of new therapy.

No MeSH data available.


Relative gene expression of p53 from the colon of CAC models. RNA was extracted from colons, and p53 gene expression was analyzed by real time PCR and normalized by β-actin mRNA. Results are representative of four independent experiments. Results are mean ± SD. Data with statistically not significant differences (ns = not significant)
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Fig6: Relative gene expression of p53 from the colon of CAC models. RNA was extracted from colons, and p53 gene expression was analyzed by real time PCR and normalized by β-actin mRNA. Results are representative of four independent experiments. Results are mean ± SD. Data with statistically not significant differences (ns = not significant)

Mentions: The AOM and DSS administration to Balb/C mice decreased the expression of p53 (p = 0.029). The expression of p53 at DSS only group decreased 49 % when compared with mice were received DPE of 125 mg/kg BW group. The expression of p53 responded to administration of DPE in a manner inversely doses dependent. The dose of DPE 125 mg group slightly increased p53 expression when it was compared with a DSS only group (p = 0.06). The difference was not statistically significant between DPE I 125 mg/kg, DPE II DPE 250 mg/kg and III DPE 500 mg/kg groups. Although there were slightly increased p53 expression in DPE I 125 mg/kg treatment group, the colons from CAC mice (Fig. 6).Fig. 6


Dendrophthoe pentandra (L.) Miq extract effectively inhibits inflammation, proliferation and induces p53 expression on colitis-associated colon cancer
Relative gene expression of p53 from the colon of CAC models. RNA was extracted from colons, and p53 gene expression was analyzed by real time PCR and normalized by β-actin mRNA. Results are representative of four independent experiments. Results are mean ± SD. Data with statistically not significant differences (ns = not significant)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5037598&req=5

Fig6: Relative gene expression of p53 from the colon of CAC models. RNA was extracted from colons, and p53 gene expression was analyzed by real time PCR and normalized by β-actin mRNA. Results are representative of four independent experiments. Results are mean ± SD. Data with statistically not significant differences (ns = not significant)
Mentions: The AOM and DSS administration to Balb/C mice decreased the expression of p53 (p = 0.029). The expression of p53 at DSS only group decreased 49 % when compared with mice were received DPE of 125 mg/kg BW group. The expression of p53 responded to administration of DPE in a manner inversely doses dependent. The dose of DPE 125 mg group slightly increased p53 expression when it was compared with a DSS only group (p = 0.06). The difference was not statistically significant between DPE I 125 mg/kg, DPE II DPE 250 mg/kg and III DPE 500 mg/kg groups. Although there were slightly increased p53 expression in DPE I 125 mg/kg treatment group, the colons from CAC mice (Fig. 6).Fig. 6

View Article: PubMed Central - PubMed

ABSTRACT

Background: Indonesian mistletoe grows on various trees. Mango Mistletoes (Dendrophthoe pentandra) is one type of mistletoe that grown on mango tree (.benalu mangga in bahasa Indonesia). Our study used mistletoe as a parasitic plant that has been used for traditional medicine. It has been known that Dendrophtoe pentandra extract (DPE) anti-inflammatory and anticancer. Furthermore, it is necessary to follow-up study in vivo to evaluate the response to treatment of new cancer therapeutic agents. This research aimed to determine the levels of IL-22, myeloperoxide (MPO), proliferation and wild-type p53 expression after the administration of DPE to murine models of CAC.

Methods: Mouse colitis associated colon cancer (CAC) was induced firstly by azoxymethane (AOM) and followed by administration of drinking water containing 5 % dextran sodium sulfate (DSS) in a cycle protocol, each cycle consisted of seven days of 5 % DSS in the drinking water and followed by seven days of regular water. This study consists of five treatment groups: I was treated water only (control), II was administrated by (DSS only, without DPE), (III-V) were administrated by DPE (125 mg/kg BW, 250 mg/kg BW and 500 mg/kg BW) respectively. The administrated of DPE were started from the 8th weeks, were continued until 21 weeks. At the end of 21 weeks of the experiment, mice were sacrificed, colon tissue was removed, and then subjected to ELISA, flow cytometry, real-time PCR and histology examination.

Results: Administration of DPE 250 mg/kgBW significantly reduce the levels of IL-22 and MPO compared with DSS only group (p < 0.001; p < 0.001). Colonic epithelial cells proliferation of group IV (DPE 250 mg/kgBW) were significantly lower than III and V groups. There was no significant change in the S phase in mice were treated DPE 125 mg/kg BW and 500 mg/kg BW, while administration of DPE 250 mg/kg BW was able to increase the percentage of cells in S phase. The expression of mRNA p53 was up regulated in mice received DPE 125 mg/kg BW.

Conclusion: These findings indicate that the DPE could inhibit colonic epithelial cells proliferation through p53 pathway independently. This study also showed that DPE could be potential sources of new therapy.

No MeSH data available.