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The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

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ABSTRACT

Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

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Related in: MedlinePlus

Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; (C) protein abundance of muscle eIF4G; and (D) representative Western blot of rats fed different types of protein for seven days after a protein restricted period. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a> b > c.
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nutrients-08-00573-f010: Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; (C) protein abundance of muscle eIF4G; and (D) representative Western blot of rats fed different types of protein for seven days after a protein restricted period. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a> b > c.

Mentions: Skeletal muscle is one of the main sites of protein synthesis. After a meal, as nutrients are absorbed, the rate of protein synthesis increases [44]. Because the Cr diet contains three times the leucine requirement, and leucine increases protein synthesis, we were interested in evaluating whether the Cr diet could modify muscle mTORC1-signaling pathway. No changes were observed in the phosphorylation of mTORC1 and S6K1 in muscle after one day of consuming the different experimental diets (Figure S1). Interestingly, after seven days of consuming the experimental diets, we observed a significant increase in the mTORC1 phosphorylation in the B and BCr groups followed by the S group. The Cr and SCr groups showed the lowest p-mTORC1/mTORC1 ratio (Figure 10A). The BCr group followed by the SCr group showed the highest (p < 0.05) p-S6K1/S6k1 ratio among the groups (Figure 10B). The protein expression of eIF4G in muscle was significantly increased in the S and BCr groups (Figure 10C).


The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein
Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; (C) protein abundance of muscle eIF4G; and (D) representative Western blot of rats fed different types of protein for seven days after a protein restricted period. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a> b > c.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f010: Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; (C) protein abundance of muscle eIF4G; and (D) representative Western blot of rats fed different types of protein for seven days after a protein restricted period. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a> b > c.
Mentions: Skeletal muscle is one of the main sites of protein synthesis. After a meal, as nutrients are absorbed, the rate of protein synthesis increases [44]. Because the Cr diet contains three times the leucine requirement, and leucine increases protein synthesis, we were interested in evaluating whether the Cr diet could modify muscle mTORC1-signaling pathway. No changes were observed in the phosphorylation of mTORC1 and S6K1 in muscle after one day of consuming the different experimental diets (Figure S1). Interestingly, after seven days of consuming the experimental diets, we observed a significant increase in the mTORC1 phosphorylation in the B and BCr groups followed by the S group. The Cr and SCr groups showed the lowest p-mTORC1/mTORC1 ratio (Figure 10A). The BCr group followed by the SCr group showed the highest (p < 0.05) p-S6K1/S6k1 ratio among the groups (Figure 10B). The protein expression of eIF4G in muscle was significantly increased in the S and BCr groups (Figure 10C).

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world&rsquo;s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus