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The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

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Related in: MedlinePlus

Hepatic polysome profile of rats fed different types of dietary proteins after a protein restricted period: (A) 0.5% casein; (B) casein; (C) soy; (D) black bean; (E) black bean + corn; (F) soy + corn; (G) corn; and (H) area under the curve analysis. The values are mean ± SEM, n = 3 per group. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
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nutrients-08-00573-f009: Hepatic polysome profile of rats fed different types of dietary proteins after a protein restricted period: (A) 0.5% casein; (B) casein; (C) soy; (D) black bean; (E) black bean + corn; (F) soy + corn; (G) corn; and (H) area under the curve analysis. The values are mean ± SEM, n = 3 per group. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.

Mentions: The results indicate that each type of dietary protein generates a specific pattern of expression and phosphorylation of factors involved in the initiation of translation leading to a specific pattern of polysome formation. In general, there was a significant increase (p < 0.05) in polysome formation in all groups in comparison with the LP group (Figure 9A–G). The polysome profiles in the livers of rats fed SCr, S and BCr showed the highest polysome formation (Figure 9H) indicating a possible increase in the initiation of protein translation.


The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein
Hepatic polysome profile of rats fed different types of dietary proteins after a protein restricted period: (A) 0.5% casein; (B) casein; (C) soy; (D) black bean; (E) black bean + corn; (F) soy + corn; (G) corn; and (H) area under the curve analysis. The values are mean ± SEM, n = 3 per group. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f009: Hepatic polysome profile of rats fed different types of dietary proteins after a protein restricted period: (A) 0.5% casein; (B) casein; (C) soy; (D) black bean; (E) black bean + corn; (F) soy + corn; (G) corn; and (H) area under the curve analysis. The values are mean ± SEM, n = 3 per group. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
Mentions: The results indicate that each type of dietary protein generates a specific pattern of expression and phosphorylation of factors involved in the initiation of translation leading to a specific pattern of polysome formation. In general, there was a significant increase (p < 0.05) in polysome formation in all groups in comparison with the LP group (Figure 9A–G). The polysome profiles in the livers of rats fed SCr, S and BCr showed the highest polysome formation (Figure 9H) indicating a possible increase in the initiation of protein translation.

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world&rsquo;s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus