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The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus

mRNA gene expression and Western blot analysis of SREBP1C and FASN in rats fed different types of proteins after a protein restricted period: (A) Srebp1c mRNA abundance; (B) Fasn mRNA abundance; (C) abundance of SREBP1c; and (D) abundance of FASN. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
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nutrients-08-00573-f008: mRNA gene expression and Western blot analysis of SREBP1C and FASN in rats fed different types of proteins after a protein restricted period: (A) Srebp1c mRNA abundance; (B) Fasn mRNA abundance; (C) abundance of SREBP1c; and (D) abundance of FASN. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.

Mentions: Srebp1c gene expression was significantly increased in the C, Cr and LP groups (p < 0.05), and, interestingly, the S group showed the lowest expression of Srebp1c (p < 0.05) (Figure 8A). SREBP1c protein expression was significantly increased (p < 0.05) in the Cr group by 2.7-fold in comparison with the LP group (Figure 8C). The gene expression of Fasn was significantly increased (p < 0.05) in the Cr group, and the rats fed S had the lowest expression of Fasn (p < 0.05) (Figure 8B). The highest protein expression of FASN was observed in the Cr group and was significantly decreased by the combination of Cr with S or B (Figure 8D).


The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein
mRNA gene expression and Western blot analysis of SREBP1C and FASN in rats fed different types of proteins after a protein restricted period: (A) Srebp1c mRNA abundance; (B) Fasn mRNA abundance; (C) abundance of SREBP1c; and (D) abundance of FASN. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f008: mRNA gene expression and Western blot analysis of SREBP1C and FASN in rats fed different types of proteins after a protein restricted period: (A) Srebp1c mRNA abundance; (B) Fasn mRNA abundance; (C) abundance of SREBP1c; and (D) abundance of FASN. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
Mentions: Srebp1c gene expression was significantly increased in the C, Cr and LP groups (p < 0.05), and, interestingly, the S group showed the lowest expression of Srebp1c (p < 0.05) (Figure 8A). SREBP1c protein expression was significantly increased (p < 0.05) in the Cr group by 2.7-fold in comparison with the LP group (Figure 8C). The gene expression of Fasn was significantly increased (p < 0.05) in the Cr group, and the rats fed S had the lowest expression of Fasn (p < 0.05) (Figure 8B). The highest protein expression of FASN was observed in the Cr group and was significantly decreased by the combination of Cr with S or B (Figure 8D).

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world&rsquo;s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus