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The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis and quantification of GCN2 and eIF2α in liver of rats fed different types of proteins after a protein restricted period: (A) representative Western blot analysis of GCN2 and (B) eIF2α; (C) protein abundance of GCN2; and (D) protein abundance of eIF2α. Values are means ± SEM, n = 3, p < 0.05, a > b > c.
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nutrients-08-00573-f007: Western blot analysis and quantification of GCN2 and eIF2α in liver of rats fed different types of proteins after a protein restricted period: (A) representative Western blot analysis of GCN2 and (B) eIF2α; (C) protein abundance of GCN2; and (D) protein abundance of eIF2α. Values are means ± SEM, n = 3, p < 0.05, a > b > c.

Mentions: Rats fed Cr showed the highest expression of GCN2 after seven and 14 days (p < 0.0001) of eating the experimental diets, while rats fed C, S, B, BCr or SCr showed the lowest expression of GCN2 (Figure 7A,C). Despite the abundance of GCN2 in all groups, we did not observed phosphorylation of its target protein eIF2α (Figure 7B,D).


The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein
Western blot analysis and quantification of GCN2 and eIF2α in liver of rats fed different types of proteins after a protein restricted period: (A) representative Western blot analysis of GCN2 and (B) eIF2α; (C) protein abundance of GCN2; and (D) protein abundance of eIF2α. Values are means ± SEM, n = 3, p < 0.05, a > b > c.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f007: Western blot analysis and quantification of GCN2 and eIF2α in liver of rats fed different types of proteins after a protein restricted period: (A) representative Western blot analysis of GCN2 and (B) eIF2α; (C) protein abundance of GCN2; and (D) protein abundance of eIF2α. Values are means ± SEM, n = 3, p < 0.05, a > b > c.
Mentions: Rats fed Cr showed the highest expression of GCN2 after seven and 14 days (p < 0.0001) of eating the experimental diets, while rats fed C, S, B, BCr or SCr showed the lowest expression of GCN2 (Figure 7A,C). Despite the abundance of GCN2 in all groups, we did not observed phosphorylation of its target protein eIF2α (Figure 7B,D).

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world&rsquo;s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus