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The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

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ABSTRACT

Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

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mRNA gene expression and Western blot analysis of SNAT2 in liver of rats fed different types of proteins after a protein restricted period: (A) Snat2 mRNA abundance; (B) protein abundance of Snat2; and (C) representative Western blot analysis. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among time, p < 0.05, a > b > c.
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nutrients-08-00573-f006: mRNA gene expression and Western blot analysis of SNAT2 in liver of rats fed different types of proteins after a protein restricted period: (A) Snat2 mRNA abundance; (B) protein abundance of Snat2; and (C) representative Western blot analysis. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among time, p < 0.05, a > b > c.

Mentions: Previous studies demonstrated that SNAT2 was upregulated in response to amino acid deprivation [42]. Thus, we evaluated SNAT2 as a possible amino acid sensor and if it could be regulated by the physiological state. The gene expression of Snat2 was significantly increased (p < 0.05) in the SCr and Cr groups after one day of eating the experimental diets (Figure 6A); interestingly after seven and 14 days of eating the experimental diets there is a significant decrease (p < 0.05) in the gene expression of Snat2 (Figure 6B) regardless the experimental diet indicating adaptive regulation, a process in which amino acid starvation induces the transport activity [43]. The protein expression of SNAT2 was significantly elevated (p < 0.05) after one day of eating the experimental diets and decreased after seven and 14 days of dietary protein rehabilitation (Figure 6C).


The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein
mRNA gene expression and Western blot analysis of SNAT2 in liver of rats fed different types of proteins after a protein restricted period: (A) Snat2 mRNA abundance; (B) protein abundance of Snat2; and (C) representative Western blot analysis. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among time, p < 0.05, a > b > c.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f006: mRNA gene expression and Western blot analysis of SNAT2 in liver of rats fed different types of proteins after a protein restricted period: (A) Snat2 mRNA abundance; (B) protein abundance of Snat2; and (C) representative Western blot analysis. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among time, p < 0.05, a > b > c.
Mentions: Previous studies demonstrated that SNAT2 was upregulated in response to amino acid deprivation [42]. Thus, we evaluated SNAT2 as a possible amino acid sensor and if it could be regulated by the physiological state. The gene expression of Snat2 was significantly increased (p < 0.05) in the SCr and Cr groups after one day of eating the experimental diets (Figure 6A); interestingly after seven and 14 days of eating the experimental diets there is a significant decrease (p < 0.05) in the gene expression of Snat2 (Figure 6B) regardless the experimental diet indicating adaptive regulation, a process in which amino acid starvation induces the transport activity [43]. The protein expression of SNAT2 was significantly elevated (p < 0.05) after one day of eating the experimental diets and decreased after seven and 14 days of dietary protein rehabilitation (Figure 6C).

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world&rsquo;s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus