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The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; and (C) protein abundance of eIF4G in the livers of rats fed different types of proteins after a protein restricted period; and (D) representative Western blot analysis. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
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nutrients-08-00573-f005: Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; and (C) protein abundance of eIF4G in the livers of rats fed different types of proteins after a protein restricted period; and (D) representative Western blot analysis. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.

Mentions: The major differences in the mTORC1-signaling pathway in the liver were observed after one day of consuming the experimental diets. The BCr group showed the highest increase in the phosphorylation of mTORC1 (p < 0.05) (Figure 5A), whereas the SCr group showed the highest pS6K1/S6K ratio (p < 0.05) (Figure 5B), and the SCr group followed by the BCr group showed the highest expression of eIF4G (p < 0.05) (Figure 5C). These results suggest that the combination of dietary proteins significantly increases the hepatic mTORC1-signaling pathway, which could be related to an increase in protein synthesis.


The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein
Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; and (C) protein abundance of eIF4G in the livers of rats fed different types of proteins after a protein restricted period; and (D) representative Western blot analysis. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f005: Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; and (C) protein abundance of eIF4G in the livers of rats fed different types of proteins after a protein restricted period; and (D) representative Western blot analysis. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
Mentions: The major differences in the mTORC1-signaling pathway in the liver were observed after one day of consuming the experimental diets. The BCr group showed the highest increase in the phosphorylation of mTORC1 (p < 0.05) (Figure 5A), whereas the SCr group showed the highest pS6K1/S6K ratio (p < 0.05) (Figure 5B), and the SCr group followed by the BCr group showed the highest expression of eIF4G (p < 0.05) (Figure 5C). These results suggest that the combination of dietary proteins significantly increases the hepatic mTORC1-signaling pathway, which could be related to an increase in protein synthesis.

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world&rsquo;s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus