Limits...
The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus

mRNA gene expression and Western blot analysis of SREBP1C and FASN in healthy rats: (A) Srebp1c and (B) Fasn mRNA abundance; (C) protein abundance of SREBP1c; and (D) FASN. Values are means ± SEM, n = 3. Different letter superscript in the bars indicates significant differences among groups, p < 0.05, a > b > c.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f004: mRNA gene expression and Western blot analysis of SREBP1C and FASN in healthy rats: (A) Srebp1c and (B) Fasn mRNA abundance; (C) protein abundance of SREBP1c; and (D) FASN. Values are means ± SEM, n = 3. Different letter superscript in the bars indicates significant differences among groups, p < 0.05, a > b > c.

Mentions: Diets low in lysine lead to fatty liver [40]. For this reason, we assessed the gene expression of the transcription factor Srebp1c, which is involved in fatty acid synthesis [41]. Srebp1c expression was significantly increased (p < 0.05) in the Cr and Se groups by 3.1- and 1.4-fold, respectively, with respect the C group. Interestingly, the S, BCr, Sp and P groups showed the lowest gene expression of Srebp1c (Figure 4A). The protein expression of SREBP1c was significantly increased (p < 0.05) in the rats fed P, Sp, Se and Cr, while the rats fed S, B and BCr showed the lowest expression of SREBP1C (Figure 4C). The fatty acid synthase gene (Fasn), the target gene of SREBP-1c, was significantly increased (p < 0.05) in the Cr group followed by the Se group after refeeding (Figure 4B). The protein expression of FASN increased in the Se and Cr groups after refeeding by 59% and 36.2%, respectively, in comparison with the C group (Figure 4D).


The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein
mRNA gene expression and Western blot analysis of SREBP1C and FASN in healthy rats: (A) Srebp1c and (B) Fasn mRNA abundance; (C) protein abundance of SREBP1c; and (D) FASN. Values are means ± SEM, n = 3. Different letter superscript in the bars indicates significant differences among groups, p < 0.05, a > b > c.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f004: mRNA gene expression and Western blot analysis of SREBP1C and FASN in healthy rats: (A) Srebp1c and (B) Fasn mRNA abundance; (C) protein abundance of SREBP1c; and (D) FASN. Values are means ± SEM, n = 3. Different letter superscript in the bars indicates significant differences among groups, p < 0.05, a > b > c.
Mentions: Diets low in lysine lead to fatty liver [40]. For this reason, we assessed the gene expression of the transcription factor Srebp1c, which is involved in fatty acid synthesis [41]. Srebp1c expression was significantly increased (p < 0.05) in the Cr and Se groups by 3.1- and 1.4-fold, respectively, with respect the C group. Interestingly, the S, BCr, Sp and P groups showed the lowest gene expression of Srebp1c (Figure 4A). The protein expression of SREBP1c was significantly increased (p < 0.05) in the rats fed P, Sp, Se and Cr, while the rats fed S, B and BCr showed the lowest expression of SREBP1C (Figure 4C). The fatty acid synthase gene (Fasn), the target gene of SREBP-1c, was significantly increased (p < 0.05) in the Cr group followed by the Se group after refeeding (Figure 4B). The protein expression of FASN increased in the Se and Cr groups after refeeding by 59% and 36.2%, respectively, in comparison with the C group (Figure 4D).

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world&rsquo;s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus