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The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus

mRNA gene expression and Western blot analysis of SNAT2 in liver of healthy rats fed different types of dietary protein: (A) Snat2 mRNA abundance; (B) protein abundance of Snat2; and (C) representative immunoblot of SNAT2. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among different times of feeding, p < 0.05, a > b > c.
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nutrients-08-00573-f003: mRNA gene expression and Western blot analysis of SNAT2 in liver of healthy rats fed different types of dietary protein: (A) Snat2 mRNA abundance; (B) protein abundance of Snat2; and (C) representative immunoblot of SNAT2. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among different times of feeding, p < 0.05, a > b > c.

Mentions: Gene and protein expression of SNAT2 was not modified by the type of dietary protein (Figure 3A,B), however it was increased during the fasting period and decreased after feeding period (inset Figure 3A,B) (p < 0.05).


The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein
mRNA gene expression and Western blot analysis of SNAT2 in liver of healthy rats fed different types of dietary protein: (A) Snat2 mRNA abundance; (B) protein abundance of Snat2; and (C) representative immunoblot of SNAT2. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among different times of feeding, p < 0.05, a > b > c.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f003: mRNA gene expression and Western blot analysis of SNAT2 in liver of healthy rats fed different types of dietary protein: (A) Snat2 mRNA abundance; (B) protein abundance of Snat2; and (C) representative immunoblot of SNAT2. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among different times of feeding, p < 0.05, a > b > c.
Mentions: Gene and protein expression of SNAT2 was not modified by the type of dietary protein (Figure 3A,B), however it was increased during the fasting period and decreased after feeding period (inset Figure 3A,B) (p < 0.05).

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world&rsquo;s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus