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The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein

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ABSTRACT

Between 6% and 11% of the world’s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

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Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; (C) protein abundance of eIF4G in livers of healthy rats fed different types of dietary proteins and (D) representative immunoblot. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
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nutrients-08-00573-f002: Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; (C) protein abundance of eIF4G in livers of healthy rats fed different types of dietary proteins and (D) representative immunoblot. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.

Mentions: After 1 h of feeding, the p-mTORC1/mTORC1 ratio was significantly increased (p < 0.05) in the S, B and BCr groups, while the Cr and P groups had lower phosphorylation of mTORC1 (Figure 2A). The BCr group had the highest p-S6K1/S6K1 ratio among the experimental groups (p < 0.05) indicating a possible increase in ribosomal biogenesis [37]. Interestingly, the Cr group exhibited the lower phosphorylation of S6K1 (Figure 2B) indicating that, although Cr is rich in leucine that can stimulate mTORC1 [38], the deficiency of lysine and tryptophan in Cr could repress the activity of S6K1. We measured the expression of eIF4G, a translation initiation factor involved in the formation of eIF4E [39], and we observed that the expression of eIF4G was significantly increased (p < 0.05) in the B, BCr and P groups; meanwhile, the S, Cr, Sp and Se groups exhibited lower expression (p < 0.05) of eIF4G (Figure 2C).


The mTORC1-Signaling Pathway and Hepatic Polyribosome Profile Are Enhanced after the Recovery of a Protein Restricted Diet by a Combination of Soy or Black Bean with Corn Protein
Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; (C) protein abundance of eIF4G in livers of healthy rats fed different types of dietary proteins and (D) representative immunoblot. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037558&req=5

nutrients-08-00573-f002: Western blot analysis and quantification of the: (A) phosphorylation of mTORC1; (B) phosphorylation of S6K1; (C) protein abundance of eIF4G in livers of healthy rats fed different types of dietary proteins and (D) representative immunoblot. Values are means ± SEM, n = 3. Different letter superscript indicates significant differences among groups, p < 0.05, a > b > c.
Mentions: After 1 h of feeding, the p-mTORC1/mTORC1 ratio was significantly increased (p < 0.05) in the S, B and BCr groups, while the Cr and P groups had lower phosphorylation of mTORC1 (Figure 2A). The BCr group had the highest p-S6K1/S6K1 ratio among the experimental groups (p < 0.05) indicating a possible increase in ribosomal biogenesis [37]. Interestingly, the Cr group exhibited the lower phosphorylation of S6K1 (Figure 2B) indicating that, although Cr is rich in leucine that can stimulate mTORC1 [38], the deficiency of lysine and tryptophan in Cr could repress the activity of S6K1. We measured the expression of eIF4G, a translation initiation factor involved in the formation of eIF4E [39], and we observed that the expression of eIF4G was significantly increased (p < 0.05) in the B, BCr and P groups; meanwhile, the S, Cr, Sp and Se groups exhibited lower expression (p < 0.05) of eIF4G (Figure 2C).

View Article: PubMed Central - PubMed

ABSTRACT

Between 6% and 11% of the world&rsquo;s population suffers from malnutrition or undernutrition associated with poverty, aging or long-term hospitalization. The present work examined the effect of different types of proteins on the mechanistic target of rapamycin (mTORC1)-signaling pathway in: (1) healthy; and (2) protein restricted rats. (1) In total, 200 rats were divided into eight groups and fed one of the following diets: 20% casein (C), soy (S), black bean (B), B + Corn (BCr), Pea (P), spirulina (Sp), sesame (Se) or Corn (Cr). Rats fed C or BCr had the highest body weight gain; rats fed BCr had the highest pS6K1/S6K1 ratio; rats fed B, BCr or P had the highest eIF4G expression; (2) In total, 84 rats were fed 0.5% C for 21 day and protein rehabilitated with different proteins. The S, soy + Corn (SCr) and BCr groups had the highest body weight gain. Rats fed SCr and BCr had the highest eIF4G expression and liver polysome formation. These findings suggest that the quality of the dietary proteins modulate the mTORC1-signaling pathway. In conclusion, the combination of BCr or SCr are the best proteins for dietary protein rehabilitation due to the significant increase in body weight, activation of the mTORC1-signaling pathway in liver and muscle, and liver polysome formation.

No MeSH data available.


Related in: MedlinePlus