Limits...
Eicosapentaenoic and Docosahexaenoic Acid-Enriched High Fat Diet Delays Skeletal Muscle Degradation in Mice

View Article: PubMed Central - PubMed

ABSTRACT

Low-grade chronic inflammatory conditions such as ageing, obesity and related metabolic disorders are associated with deterioration of skeletal muscle (SkM). Human studies have shown that marine fatty acids influence SkM function, though the underlying mechanisms of action are unknown. As a model of diet-induced obesity, we fed C57BL/6J mice either a high fat diet (HFD) with purified marine fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (HFD-ED), a HFD with corn oil, or normal mouse chow for 8 weeks; and used transcriptomics to identify the molecular effects of EPA and DHA on SkM. Consumption of ED-enriched HFD modulated SkM metabolism through increased gene expression of mitochondrial β-oxidation and slow-fiber type genes compared with HFD-corn oil fed mice. Furthermore, HFD-ED intake increased nuclear localization of nuclear factor of activated T-cells (Nfatc4) protein, which controls fiber-type composition. This data suggests a role for EPA and DHA in mitigating some of the molecular responses due to a HFD in SkM. Overall, the results suggest that increased consumption of the marine fatty acids EPA and DHA may aid in the prevention of molecular processes that lead to muscle deterioration commonly associated with obesity-induced low-grade inflammation.

No MeSH data available.


Related in: MedlinePlus

Pathway analysis of the gSkM transcriptome for the comparison HFD-ED versus HFD-corn oil fed mice. The illustration is based on the Kegg pathway database (http://www.genome.jp/kegg/pathway.html). (a) Nerve-Muscle contraction: Genes highlighted in red are upregulated in gSkM of mice fed HFD-ED compared with HFD-corn oil; (b) Relative Troponin C1 protein level in HFD-corn oil (black bars) fed mice was lower than HFD-ED (white bars). * Different at p < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5037530&req=5

nutrients-08-00543-f002: Pathway analysis of the gSkM transcriptome for the comparison HFD-ED versus HFD-corn oil fed mice. The illustration is based on the Kegg pathway database (http://www.genome.jp/kegg/pathway.html). (a) Nerve-Muscle contraction: Genes highlighted in red are upregulated in gSkM of mice fed HFD-ED compared with HFD-corn oil; (b) Relative Troponin C1 protein level in HFD-corn oil (black bars) fed mice was lower than HFD-ED (white bars). * Different at p < 0.05.

Mentions: Skeletal muscle fibers require stimulation from the neuromuscular junctions acting on the cholinergic nicotine receptor on the muscle cells to initiate contraction. These efferent cholinergic nerves are crucial for voluntary control of skeletal muscles. Gene expression of the cholinergic receptor nicotinic α polypeptide 1 (Chrna1), insulin growth factor receptor (Igfr) and two l-type voltage dependent Ca-channel genes (Cacna1s and Cacnb2), two calcium ATPases (Atp1a1 and Atp2a2, a.k.a Serca 1 and 2, respectively), were upregulated by HFD-ED compared with HFD-corn oil fed mice (Figure 2a).


Eicosapentaenoic and Docosahexaenoic Acid-Enriched High Fat Diet Delays Skeletal Muscle Degradation in Mice
Pathway analysis of the gSkM transcriptome for the comparison HFD-ED versus HFD-corn oil fed mice. The illustration is based on the Kegg pathway database (http://www.genome.jp/kegg/pathway.html). (a) Nerve-Muscle contraction: Genes highlighted in red are upregulated in gSkM of mice fed HFD-ED compared with HFD-corn oil; (b) Relative Troponin C1 protein level in HFD-corn oil (black bars) fed mice was lower than HFD-ED (white bars). * Different at p < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037530&req=5

nutrients-08-00543-f002: Pathway analysis of the gSkM transcriptome for the comparison HFD-ED versus HFD-corn oil fed mice. The illustration is based on the Kegg pathway database (http://www.genome.jp/kegg/pathway.html). (a) Nerve-Muscle contraction: Genes highlighted in red are upregulated in gSkM of mice fed HFD-ED compared with HFD-corn oil; (b) Relative Troponin C1 protein level in HFD-corn oil (black bars) fed mice was lower than HFD-ED (white bars). * Different at p < 0.05.
Mentions: Skeletal muscle fibers require stimulation from the neuromuscular junctions acting on the cholinergic nicotine receptor on the muscle cells to initiate contraction. These efferent cholinergic nerves are crucial for voluntary control of skeletal muscles. Gene expression of the cholinergic receptor nicotinic α polypeptide 1 (Chrna1), insulin growth factor receptor (Igfr) and two l-type voltage dependent Ca-channel genes (Cacna1s and Cacnb2), two calcium ATPases (Atp1a1 and Atp2a2, a.k.a Serca 1 and 2, respectively), were upregulated by HFD-ED compared with HFD-corn oil fed mice (Figure 2a).

View Article: PubMed Central - PubMed

ABSTRACT

Low-grade chronic inflammatory conditions such as ageing, obesity and related metabolic disorders are associated with deterioration of skeletal muscle (SkM). Human studies have shown that marine fatty acids influence SkM function, though the underlying mechanisms of action are unknown. As a model of diet-induced obesity, we fed C57BL/6J mice either a high fat diet (HFD) with purified marine fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (HFD-ED), a HFD with corn oil, or normal mouse chow for 8 weeks; and used transcriptomics to identify the molecular effects of EPA and DHA on SkM. Consumption of ED-enriched HFD modulated SkM metabolism through increased gene expression of mitochondrial &beta;-oxidation and slow-fiber type genes compared with HFD-corn oil fed mice. Furthermore, HFD-ED intake increased nuclear localization of nuclear factor of activated T-cells (Nfatc4) protein, which controls fiber-type composition. This data suggests a role for EPA and DHA in mitigating some of the molecular responses due to a HFD in SkM. Overall, the results suggest that increased consumption of the marine fatty acids EPA and DHA may aid in the prevention of molecular processes that lead to muscle deterioration commonly associated with obesity-induced low-grade inflammation.

No MeSH data available.


Related in: MedlinePlus