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Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk

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ABSTRACT

The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was performed using SDS-PAGE and SE-specific ELISA, and amino acid analysis was used to assign its protein concentration. The solution was then used to prepare six PT materials (four milk and two buffer batches) at a ng/g toxin level, which included one blank and one SEA-containing milk as specificity control. Suitable material homogeneity and stability were assessed using screening and quantitative ELISAs. Among the methods used by the participants, ELISA-based methods demonstrated their efficiency for the detection of SEB in both simple and complex matrices. The results serve as a basis for further improving the detection capabilities in expert laboratories and can therefore be considered as a contribution to biopreparedness.

No MeSH data available.


Homogeneity data obtained for the buffer (B1 and B2) and milk (M2 and M3) samples spiked by SEB. (a): in buffer (B1), (b): in buffer (B2), (c): in milk (M2), (d): in milk (M3).
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toxins-08-00268-f002: Homogeneity data obtained for the buffer (B1 and B2) and milk (M2 and M3) samples spiked by SEB. (a): in buffer (B1), (b): in buffer (B2), (c): in milk (M2), (d): in milk (M3).

Mentions: Homogeneity data for buffer (B1 and B2) and milk (M2 and M3) samples spiked with SEB are presented in Figure 2.


Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk
Homogeneity data obtained for the buffer (B1 and B2) and milk (M2 and M3) samples spiked by SEB. (a): in buffer (B1), (b): in buffer (B2), (c): in milk (M2), (d): in milk (M3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037494&req=5

toxins-08-00268-f002: Homogeneity data obtained for the buffer (B1 and B2) and milk (M2 and M3) samples spiked by SEB. (a): in buffer (B1), (b): in buffer (B2), (c): in milk (M2), (d): in milk (M3).
Mentions: Homogeneity data for buffer (B1 and B2) and milk (M2 and M3) samples spiked with SEB are presented in Figure 2.

View Article: PubMed Central - PubMed

ABSTRACT

The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was performed using SDS-PAGE and SE-specific ELISA, and amino acid analysis was used to assign its protein concentration. The solution was then used to prepare six PT materials (four milk and two buffer batches) at a ng/g toxin level, which included one blank and one SEA-containing milk as specificity control. Suitable material homogeneity and stability were assessed using screening and quantitative ELISAs. Among the methods used by the participants, ELISA-based methods demonstrated their efficiency for the detection of SEB in both simple and complex matrices. The results serve as a basis for further improving the detection capabilities in expert laboratories and can therefore be considered as a contribution to biopreparedness.

No MeSH data available.