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The Contents of Ustiloxins A and B along with Their Distribution in Rice False Smut Balls

View Article: PubMed Central - PubMed

ABSTRACT

Ustiloxins are cyclopeptide mycotoxins isolated from rice false smut balls (FSBs), the ball-like colonies transformed from the individual grains through the filament infection by the fungal pathogen Villosiclava virens. There were no obvious relations between ustiloxin content and any of the collection areas, collection times, or average weight of each FSB. The rice false smut balls at early, middle, and late maturity stages were respectively divided into different parts (glume, chlamydospores, mycelia, and pseudoparenchyma). The highest content of ustiloxins A and B of rice FSBs was found at the early maturity stage. Both ustiloxins A and B were mainly distributed in the middle layer containing mycelia and immature chlamydospores of the FSBs. When the rice FSBs were at the early maturity stage, the total yield of ustiloxins A and B in the middle layer of each ball was 48.3 µg, which was 3.20-fold of the yield (15.1 µg) of the inner part of the ball. The rice FSBs at the early maturity stage are the appropriate materials for the production of ustiloxins A and B.

No MeSH data available.


HPLC analysis of ustiloxins A and B in rice FSBs. (a) HPLC profile of the crude extract from rice FSBs at the early maturity stage. Arabic numerals 1 and 2 in the figure represent ustiloxins A and B, respectively; (b) HPLC profile of the standard ustiloxins B and A. The retention times of ustiloxins B and A were 8.4 and 16.0 min, respectively.
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toxins-08-00262-f002: HPLC analysis of ustiloxins A and B in rice FSBs. (a) HPLC profile of the crude extract from rice FSBs at the early maturity stage. Arabic numerals 1 and 2 in the figure represent ustiloxins A and B, respectively; (b) HPLC profile of the standard ustiloxins B and A. The retention times of ustiloxins B and A were 8.4 and 16.0 min, respectively.

Mentions: The method validation including precision, accuracy, limits of detection, and quantification on HPLC analysis of ustiloxins A and B in samples has been described in our previous report [14]. The HPLC conditions in this study were consistent with those reported previously [14]. The calibration curves for ustiloxin A was Y = 1478.4X + 207,672, R2 = 0.9998, and, for ustiloxin B, Y = 1165.9X + 387,106, R2 = 0.9996, where Y was the peak area, and X was the injection quality (µg) of analyte. The results showed good linearity for the range of 0.045–7.50 µg in the sample injected. Figure 2 shows the HPLC profiles of the crude extract of rice FSBs and standard ustiloxins A and B, respectively. The main ustiloxins A and B in the crude extract were identified by comparison of their retention times with the authentic ustiloxins as well as the UV absorption spectra. HPLC analysis was completed in 25 min.


The Contents of Ustiloxins A and B along with Their Distribution in Rice False Smut Balls
HPLC analysis of ustiloxins A and B in rice FSBs. (a) HPLC profile of the crude extract from rice FSBs at the early maturity stage. Arabic numerals 1 and 2 in the figure represent ustiloxins A and B, respectively; (b) HPLC profile of the standard ustiloxins B and A. The retention times of ustiloxins B and A were 8.4 and 16.0 min, respectively.
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Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037488&req=5

toxins-08-00262-f002: HPLC analysis of ustiloxins A and B in rice FSBs. (a) HPLC profile of the crude extract from rice FSBs at the early maturity stage. Arabic numerals 1 and 2 in the figure represent ustiloxins A and B, respectively; (b) HPLC profile of the standard ustiloxins B and A. The retention times of ustiloxins B and A were 8.4 and 16.0 min, respectively.
Mentions: The method validation including precision, accuracy, limits of detection, and quantification on HPLC analysis of ustiloxins A and B in samples has been described in our previous report [14]. The HPLC conditions in this study were consistent with those reported previously [14]. The calibration curves for ustiloxin A was Y = 1478.4X + 207,672, R2 = 0.9998, and, for ustiloxin B, Y = 1165.9X + 387,106, R2 = 0.9996, where Y was the peak area, and X was the injection quality (µg) of analyte. The results showed good linearity for the range of 0.045–7.50 µg in the sample injected. Figure 2 shows the HPLC profiles of the crude extract of rice FSBs and standard ustiloxins A and B, respectively. The main ustiloxins A and B in the crude extract were identified by comparison of their retention times with the authentic ustiloxins as well as the UV absorption spectra. HPLC analysis was completed in 25 min.

View Article: PubMed Central - PubMed

ABSTRACT

Ustiloxins are cyclopeptide mycotoxins isolated from rice false smut balls (FSBs), the ball-like colonies transformed from the individual grains through the filament infection by the fungal pathogen Villosiclava virens. There were no obvious relations between ustiloxin content and any of the collection areas, collection times, or average weight of each FSB. The rice false smut balls at early, middle, and late maturity stages were respectively divided into different parts (glume, chlamydospores, mycelia, and pseudoparenchyma). The highest content of ustiloxins A and B of rice FSBs was found at the early maturity stage. Both ustiloxins A and B were mainly distributed in the middle layer containing mycelia and immature chlamydospores of the FSBs. When the rice FSBs were at the early maturity stage, the total yield of ustiloxins A and B in the middle layer of each ball was 48.3 µg, which was 3.20-fold of the yield (15.1 µg) of the inner part of the ball. The rice FSBs at the early maturity stage are the appropriate materials for the production of ustiloxins A and B.

No MeSH data available.