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Reproductive switching analysis of Daphnia similoides between sexual female and parthenogenetic female by transcriptome comparison

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ABSTRACT

The water flea Daphnia are planktonic crustaceans commonly found in freshwater environment that can switch their reproduction mode from parthenogenesis to sexual reproduction to adapt to the external environment. As such, Daphnia are great model organisms to study the mechanism of reproductive switching, the underlying mechanism of reproduction and development in cladocerans and other animals. However, little is known about the Daphnia’s reproductive behaviour at a molecular level. We constructed a genetic database of the genes expressed in a sexual female (SF) and a parthenogenetic female (PF) of D. similoides using Illumina HiSeq 2500. A total of 1,763 differentially expressed genes (865 up- and 898 down-regulated) were detected in SF. Of the top 30 up-regulated SF unigenes, the top 4 unigenes belonged to the Chitin_bind_4 family. In contrast, of the top down-regulated SF unigenes, the top 3 unigenes belonged to the Vitellogenin_N family. This is the first study to indicate genes that may have a crucial role in reproductive switching of D. similoides, which could be used as candidate genes for further functional studies. Thus, this study provides a rich resource for investigation and elucidation of reproductive switching in D. similoides.

No MeSH data available.


qPCR results of differentially expressed genes in SF and PF.SF: sexual female, PF: parthenogenetic female. The relative expression level is indicated as mean ± SE (N = 3). “*” Means significant difference between SF and PF (P < 0.05, Student t-test).
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f7: qPCR results of differentially expressed genes in SF and PF.SF: sexual female, PF: parthenogenetic female. The relative expression level is indicated as mean ± SE (N = 3). “*” Means significant difference between SF and PF (P < 0.05, Student t-test).

Mentions: In order to validate the transcriptome results, we randomly selected 14 significant DEGs from Table 1 for quantitative real-time PCR (qPCR) conformation. The primers used for qPCR are shown in Supplementary Table S2 available. The results of the qPCR were consistent with the RNA-seq data (Fig. 7).


Reproductive switching analysis of Daphnia similoides between sexual female and parthenogenetic female by transcriptome comparison
qPCR results of differentially expressed genes in SF and PF.SF: sexual female, PF: parthenogenetic female. The relative expression level is indicated as mean ± SE (N = 3). “*” Means significant difference between SF and PF (P < 0.05, Student t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037449&req=5

f7: qPCR results of differentially expressed genes in SF and PF.SF: sexual female, PF: parthenogenetic female. The relative expression level is indicated as mean ± SE (N = 3). “*” Means significant difference between SF and PF (P < 0.05, Student t-test).
Mentions: In order to validate the transcriptome results, we randomly selected 14 significant DEGs from Table 1 for quantitative real-time PCR (qPCR) conformation. The primers used for qPCR are shown in Supplementary Table S2 available. The results of the qPCR were consistent with the RNA-seq data (Fig. 7).

View Article: PubMed Central - PubMed

ABSTRACT

The water flea Daphnia are planktonic crustaceans commonly found in freshwater environment that can switch their reproduction mode from parthenogenesis to sexual reproduction to adapt to the external environment. As such, Daphnia are great model organisms to study the mechanism of reproductive switching, the underlying mechanism of reproduction and development in cladocerans and other animals. However, little is known about the Daphnia&rsquo;s reproductive behaviour at a molecular level. We constructed a genetic database of the genes expressed in a sexual female (SF) and a parthenogenetic female (PF) of D. similoides using Illumina HiSeq 2500. A total of 1,763 differentially expressed genes (865 up- and 898 down-regulated) were detected in SF. Of the top 30 up-regulated SF unigenes, the top 4 unigenes belonged to the Chitin_bind_4 family. In contrast, of the top down-regulated SF unigenes, the top 3 unigenes belonged to the Vitellogenin_N family. This is the first study to indicate genes that may have a crucial role in reproductive switching of D. similoides, which could be used as candidate genes for further functional studies. Thus, this study provides a rich resource for investigation and elucidation of reproductive switching in D. similoides.

No MeSH data available.