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Reproductive switching analysis of Daphnia similoides between sexual female and parthenogenetic female by transcriptome comparison

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ABSTRACT

The water flea Daphnia are planktonic crustaceans commonly found in freshwater environment that can switch their reproduction mode from parthenogenesis to sexual reproduction to adapt to the external environment. As such, Daphnia are great model organisms to study the mechanism of reproductive switching, the underlying mechanism of reproduction and development in cladocerans and other animals. However, little is known about the Daphnia’s reproductive behaviour at a molecular level. We constructed a genetic database of the genes expressed in a sexual female (SF) and a parthenogenetic female (PF) of D. similoides using Illumina HiSeq 2500. A total of 1,763 differentially expressed genes (865 up- and 898 down-regulated) were detected in SF. Of the top 30 up-regulated SF unigenes, the top 4 unigenes belonged to the Chitin_bind_4 family. In contrast, of the top down-regulated SF unigenes, the top 3 unigenes belonged to the Vitellogenin_N family. This is the first study to indicate genes that may have a crucial role in reproductive switching of D. similoides, which could be used as candidate genes for further functional studies. Thus, this study provides a rich resource for investigation and elucidation of reproductive switching in D. similoides.

No MeSH data available.


GO enrichment analysis of differentially expressed genes in SF and PF.BP: Biological Process, MF: Molecular Function, CC: Cellular Component, SF: sexual female, PF: parthenogenetic female. (A) SF vs PF up-regulated genes; (B) SF vs PF down-regulated genes.
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f6: GO enrichment analysis of differentially expressed genes in SF and PF.BP: Biological Process, MF: Molecular Function, CC: Cellular Component, SF: sexual female, PF: parthenogenetic female. (A) SF vs PF up-regulated genes; (B) SF vs PF down-regulated genes.

Mentions: To compare the functions of these DEGs in SF and PF, we conducted GO enrichment analysis of the identified 1,763 DEGs with the threshold value for corrected P < 0.05 (Fig. 6). The results of the GO enrichment analysis showed that all up-regulated genes (SF vs. PF) were mostly enriched in the extracellular region, peptidase activity, proteolysis, structural molecule activity, and endopeptidase activity GO processes. However, all down-regulated (SF vs. PF) genes were mostly enriched in the cellular process, cell, cell part, intracellular part, and organic cyclic compound binding GO processes.


Reproductive switching analysis of Daphnia similoides between sexual female and parthenogenetic female by transcriptome comparison
GO enrichment analysis of differentially expressed genes in SF and PF.BP: Biological Process, MF: Molecular Function, CC: Cellular Component, SF: sexual female, PF: parthenogenetic female. (A) SF vs PF up-regulated genes; (B) SF vs PF down-regulated genes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037449&req=5

f6: GO enrichment analysis of differentially expressed genes in SF and PF.BP: Biological Process, MF: Molecular Function, CC: Cellular Component, SF: sexual female, PF: parthenogenetic female. (A) SF vs PF up-regulated genes; (B) SF vs PF down-regulated genes.
Mentions: To compare the functions of these DEGs in SF and PF, we conducted GO enrichment analysis of the identified 1,763 DEGs with the threshold value for corrected P < 0.05 (Fig. 6). The results of the GO enrichment analysis showed that all up-regulated genes (SF vs. PF) were mostly enriched in the extracellular region, peptidase activity, proteolysis, structural molecule activity, and endopeptidase activity GO processes. However, all down-regulated (SF vs. PF) genes were mostly enriched in the cellular process, cell, cell part, intracellular part, and organic cyclic compound binding GO processes.

View Article: PubMed Central - PubMed

ABSTRACT

The water flea Daphnia are planktonic crustaceans commonly found in freshwater environment that can switch their reproduction mode from parthenogenesis to sexual reproduction to adapt to the external environment. As such, Daphnia are great model organisms to study the mechanism of reproductive switching, the underlying mechanism of reproduction and development in cladocerans and other animals. However, little is known about the Daphnia&rsquo;s reproductive behaviour at a molecular level. We constructed a genetic database of the genes expressed in a sexual female (SF) and a parthenogenetic female (PF) of D. similoides using Illumina HiSeq 2500. A total of 1,763 differentially expressed genes (865 up- and 898 down-regulated) were detected in SF. Of the top 30 up-regulated SF unigenes, the top 4 unigenes belonged to the Chitin_bind_4 family. In contrast, of the top down-regulated SF unigenes, the top 3 unigenes belonged to the Vitellogenin_N family. This is the first study to indicate genes that may have a crucial role in reproductive switching of D. similoides, which could be used as candidate genes for further functional studies. Thus, this study provides a rich resource for investigation and elucidation of reproductive switching in D. similoides.

No MeSH data available.