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Reproductive switching analysis of Daphnia similoides between sexual female and parthenogenetic female by transcriptome comparison

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ABSTRACT

The water flea Daphnia are planktonic crustaceans commonly found in freshwater environment that can switch their reproduction mode from parthenogenesis to sexual reproduction to adapt to the external environment. As such, Daphnia are great model organisms to study the mechanism of reproductive switching, the underlying mechanism of reproduction and development in cladocerans and other animals. However, little is known about the Daphnia’s reproductive behaviour at a molecular level. We constructed a genetic database of the genes expressed in a sexual female (SF) and a parthenogenetic female (PF) of D. similoides using Illumina HiSeq 2500. A total of 1,763 differentially expressed genes (865 up- and 898 down-regulated) were detected in SF. Of the top 30 up-regulated SF unigenes, the top 4 unigenes belonged to the Chitin_bind_4 family. In contrast, of the top down-regulated SF unigenes, the top 3 unigenes belonged to the Vitellogenin_N family. This is the first study to indicate genes that may have a crucial role in reproductive switching of D. similoides, which could be used as candidate genes for further functional studies. Thus, this study provides a rich resource for investigation and elucidation of reproductive switching in D. similoides.

No MeSH data available.


Related in: MedlinePlus

Percentage of homologous hits of the D. similoides transcripts to other species.The D. similoides transcripts were searched by Blastx against the non-redundancy protein database with a cutoff E-value 10−5.
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f3: Percentage of homologous hits of the D. similoides transcripts to other species.The D. similoides transcripts were searched by Blastx against the non-redundancy protein database with a cutoff E-value 10−5.

Mentions: Among the 37,385 unigenes, 13,072 were matched by the BLASTX homology search to the entries in NCBI non-redundant (Nr) protein database with a cut-off E-value of 10−5. The highest percentage of matching bases (88.30%) was to the D. pulex sequences, followed by the sequences of Zootermopsis nevadensis (0.80%), D. magna (0.60%), Tribolium castaneum (0.50%), and Trichuris suis (0.40%) (Fig. 3).


Reproductive switching analysis of Daphnia similoides between sexual female and parthenogenetic female by transcriptome comparison
Percentage of homologous hits of the D. similoides transcripts to other species.The D. similoides transcripts were searched by Blastx against the non-redundancy protein database with a cutoff E-value 10−5.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037449&req=5

f3: Percentage of homologous hits of the D. similoides transcripts to other species.The D. similoides transcripts were searched by Blastx against the non-redundancy protein database with a cutoff E-value 10−5.
Mentions: Among the 37,385 unigenes, 13,072 were matched by the BLASTX homology search to the entries in NCBI non-redundant (Nr) protein database with a cut-off E-value of 10−5. The highest percentage of matching bases (88.30%) was to the D. pulex sequences, followed by the sequences of Zootermopsis nevadensis (0.80%), D. magna (0.60%), Tribolium castaneum (0.50%), and Trichuris suis (0.40%) (Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

The water flea Daphnia are planktonic crustaceans commonly found in freshwater environment that can switch their reproduction mode from parthenogenesis to sexual reproduction to adapt to the external environment. As such, Daphnia are great model organisms to study the mechanism of reproductive switching, the underlying mechanism of reproduction and development in cladocerans and other animals. However, little is known about the Daphnia’s reproductive behaviour at a molecular level. We constructed a genetic database of the genes expressed in a sexual female (SF) and a parthenogenetic female (PF) of D. similoides using Illumina HiSeq 2500. A total of 1,763 differentially expressed genes (865 up- and 898 down-regulated) were detected in SF. Of the top 30 up-regulated SF unigenes, the top 4 unigenes belonged to the Chitin_bind_4 family. In contrast, of the top down-regulated SF unigenes, the top 3 unigenes belonged to the Vitellogenin_N family. This is the first study to indicate genes that may have a crucial role in reproductive switching of D. similoides, which could be used as candidate genes for further functional studies. Thus, this study provides a rich resource for investigation and elucidation of reproductive switching in D. similoides.

No MeSH data available.


Related in: MedlinePlus