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FAMSA: Fast and accurate multiple sequence alignment of huge protein families

View Article: PubMed Central - PubMed

ABSTRACT

Rapid development of modern sequencing platforms has contributed to the unprecedented growth of protein families databases. The abundance of sets containing hundreds of thousands of sequences is a formidable challenge for multiple sequence alignment algorithms. The article introduces FAMSA, a new progressive algorithm designed for fast and accurate alignment of thousands of protein sequences. Its features include the utilization of the longest common subsequence measure for determining pairwise similarities, a novel method of evaluating gap costs, and a new iterative refinement scheme. What matters is that its implementation is highly optimized and parallelized to make the most of modern computer platforms. Thanks to the above, quality indicators, i.e. sum-of-pairs and total-column scores, show FAMSA to be superior to competing algorithms, such as Clustal Omega or MAFFT for datasets exceeding a few thousand sequences. Quality does not compromise on time or memory requirements, which are an order of magnitude lower than those in the existing solutions. For example, a family of 415519 sequences was analyzed in less than two hours and required no more than 8 GB of RAM. FAMSA is available for free at http://sun.aei.polsl.pl/REFRESH/famsa.

No MeSH data available.


Example of how gap columns are inserted during profile alignment.
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f2: Example of how gap columns are inserted during profile alignment.

Mentions: Figure 2 demonstrates an example of the alignment of two profiles, X and Y. A column of gaps is to be inserted into profile X (the left part of the figure). The proper types of gaps together with the corrected gaps in the neighboring column are shown in the right part of the figure. The following situations must be considered during correction of the gaps:


FAMSA: Fast and accurate multiple sequence alignment of huge protein families
Example of how gap columns are inserted during profile alignment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037421&req=5

f2: Example of how gap columns are inserted during profile alignment.
Mentions: Figure 2 demonstrates an example of the alignment of two profiles, X and Y. A column of gaps is to be inserted into profile X (the left part of the figure). The proper types of gaps together with the corrected gaps in the neighboring column are shown in the right part of the figure. The following situations must be considered during correction of the gaps:

View Article: PubMed Central - PubMed

ABSTRACT

Rapid development of modern sequencing platforms has contributed to the unprecedented growth of protein families databases. The abundance of sets containing hundreds of thousands of sequences is a formidable challenge for multiple sequence alignment algorithms. The article introduces FAMSA, a new progressive algorithm designed for fast and accurate alignment of thousands of protein sequences. Its features include the utilization of the longest common subsequence measure for determining pairwise similarities, a novel method of evaluating gap costs, and a new iterative refinement scheme. What matters is that its implementation is highly optimized and parallelized to make the most of modern computer platforms. Thanks to the above, quality indicators, i.e. sum-of-pairs and total-column scores, show FAMSA to be superior to competing algorithms, such as Clustal Omega or MAFFT for datasets exceeding a few thousand sequences. Quality does not compromise on time or memory requirements, which are an order of magnitude lower than those in the existing solutions. For example, a family of 415519 sequences was analyzed in less than two hours and required no more than 8 GB of RAM. FAMSA is available for free at http://sun.aei.polsl.pl/REFRESH/famsa.

No MeSH data available.