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Displacement correlations between a single mesenchymal-like cell and its nucleus effectively link subcellular activities and motility in cell migration analysis

View Article: PubMed Central - PubMed

ABSTRACT

Cell migration is an essential process in organism development and physiological maintenance. Although current methods permit accurate comparisons of the effects of molecular manipulations and drug applications on cell motility, effects of alterations in subcellular activities on motility cannot be fully elucidated from those methods. Here, we develop a strategy termed cell-nuclear (CN) correlation to parameterize represented dynamic subcellular activities and to quantify their contributions in mesenchymal-like migration. Based on the biophysical meaning of the CN correlation, we propose a cell migration potential index (CMPI) to measure cell motility. When the effectiveness of CMPI was evaluated with respect to one of the most popular cell migration analysis methods, Persistent Random Walk, we found that the cell motility estimates among six cell lines used in this study were highly consistent between these two approaches. Further evaluations indicated that CMPI can be determined using a shorter time period and smaller cell sample size, and it possesses excellent reliability and applicability, even in the presence of a wide range of noise, as might be generated from individual imaging acquisition systems. The novel approach outlined here introduces a robust strategy through an analysis of subcellular locomotion activities for single cell migration assessment.

No MeSH data available.


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Specific barcodes identify significant locomotion events during the cell migration.In each row, a barcode, fluorescent image, and the trajectories of the cell and its nucleus are depicted in the left, middle, and right panels, respectively. Left: Each barcode, which displays the analysis results from its cell migration movie, was obtained from 1-min time intervals and possesses a dominant CCD-NCD// relationship in one of the four regions of the CN correlation. The percentage of the individual bars in the barcode representing each region is showed in the right column. The averaged occurrence of a region overall (50 barcodes) is shown inside the parenthesis. Middle: On the left, the cell boundaries from the 1st, 21st, 41st, and 61st fluorescent cell images are overlaid. Black markers represent the nuclear centroid positions. Red arrows indicate the migration directions of individual cells. On the right, heat map marks the distributions of cell territories in all 61 frames of the movie. The hot-to-cold color gradient illustrates the density of cell area occupancies. Right: A logarithm-logarithm plot of the overlapping mean squared displacement of the cells (red) and nuclei (blue) vs. time lag. Inset: one-hour cell and coupled nucleus trajectories of NIH 3T3 fibroblasts, colored in red and blue, respectively.
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f2: Specific barcodes identify significant locomotion events during the cell migration.In each row, a barcode, fluorescent image, and the trajectories of the cell and its nucleus are depicted in the left, middle, and right panels, respectively. Left: Each barcode, which displays the analysis results from its cell migration movie, was obtained from 1-min time intervals and possesses a dominant CCD-NCD// relationship in one of the four regions of the CN correlation. The percentage of the individual bars in the barcode representing each region is showed in the right column. The averaged occurrence of a region overall (50 barcodes) is shown inside the parenthesis. Middle: On the left, the cell boundaries from the 1st, 21st, 41st, and 61st fluorescent cell images are overlaid. Black markers represent the nuclear centroid positions. Red arrows indicate the migration directions of individual cells. On the right, heat map marks the distributions of cell territories in all 61 frames of the movie. The hot-to-cold color gradient illustrates the density of cell area occupancies. Right: A logarithm-logarithm plot of the overlapping mean squared displacement of the cells (red) and nuclei (blue) vs. time lag. Inset: one-hour cell and coupled nucleus trajectories of NIH 3T3 fibroblasts, colored in red and blue, respectively.

Mentions: To test the hypothesis discussed above, we designed a barcode to display the migration information of a single cell (Fig. 1c). Movies of a single cell and its coupled nucleus were simultaneously acquired from RFP-introduced and Hoechst 33342-labeled NIH 3T3 fibroblast cultures at 1-min intervals over one hour, and were analyzed together to extract 60 CN correlation data points. Then, each CN correlation data point was assigned to a bar with a designated color based on the location of the point in the CCD vs. NCD// plot, where Regions I, II, III, and IV were specified as red, yellow, blue, and green, respectively. The bars were plotted horizontally by the time of occurrence and grouped vertically by region to assemble a barcode. Following, we analyzed 50 NIH 3T3 fibroblast movies, and obtained corresponding barcodes of each movie as a barcode collection (Supplementary Information 1). Using this collection, we calculated the overall occurrence rate of each region in all 50 samples, where occurrence percentage rates of Regions I, II, III, and IV were 24%, 42%, 24% and 11%, respectively. Consequently, we selected four individual barcodes in each separate region (Fig. 2, left), which possesses significantly higher percentile of CN correlation occurrence than the average occurrence rate of the corresponding region, and inspected their source movies.


Displacement correlations between a single mesenchymal-like cell and its nucleus effectively link subcellular activities and motility in cell migration analysis
Specific barcodes identify significant locomotion events during the cell migration.In each row, a barcode, fluorescent image, and the trajectories of the cell and its nucleus are depicted in the left, middle, and right panels, respectively. Left: Each barcode, which displays the analysis results from its cell migration movie, was obtained from 1-min time intervals and possesses a dominant CCD-NCD// relationship in one of the four regions of the CN correlation. The percentage of the individual bars in the barcode representing each region is showed in the right column. The averaged occurrence of a region overall (50 barcodes) is shown inside the parenthesis. Middle: On the left, the cell boundaries from the 1st, 21st, 41st, and 61st fluorescent cell images are overlaid. Black markers represent the nuclear centroid positions. Red arrows indicate the migration directions of individual cells. On the right, heat map marks the distributions of cell territories in all 61 frames of the movie. The hot-to-cold color gradient illustrates the density of cell area occupancies. Right: A logarithm-logarithm plot of the overlapping mean squared displacement of the cells (red) and nuclei (blue) vs. time lag. Inset: one-hour cell and coupled nucleus trajectories of NIH 3T3 fibroblasts, colored in red and blue, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5037420&req=5

f2: Specific barcodes identify significant locomotion events during the cell migration.In each row, a barcode, fluorescent image, and the trajectories of the cell and its nucleus are depicted in the left, middle, and right panels, respectively. Left: Each barcode, which displays the analysis results from its cell migration movie, was obtained from 1-min time intervals and possesses a dominant CCD-NCD// relationship in one of the four regions of the CN correlation. The percentage of the individual bars in the barcode representing each region is showed in the right column. The averaged occurrence of a region overall (50 barcodes) is shown inside the parenthesis. Middle: On the left, the cell boundaries from the 1st, 21st, 41st, and 61st fluorescent cell images are overlaid. Black markers represent the nuclear centroid positions. Red arrows indicate the migration directions of individual cells. On the right, heat map marks the distributions of cell territories in all 61 frames of the movie. The hot-to-cold color gradient illustrates the density of cell area occupancies. Right: A logarithm-logarithm plot of the overlapping mean squared displacement of the cells (red) and nuclei (blue) vs. time lag. Inset: one-hour cell and coupled nucleus trajectories of NIH 3T3 fibroblasts, colored in red and blue, respectively.
Mentions: To test the hypothesis discussed above, we designed a barcode to display the migration information of a single cell (Fig. 1c). Movies of a single cell and its coupled nucleus were simultaneously acquired from RFP-introduced and Hoechst 33342-labeled NIH 3T3 fibroblast cultures at 1-min intervals over one hour, and were analyzed together to extract 60 CN correlation data points. Then, each CN correlation data point was assigned to a bar with a designated color based on the location of the point in the CCD vs. NCD// plot, where Regions I, II, III, and IV were specified as red, yellow, blue, and green, respectively. The bars were plotted horizontally by the time of occurrence and grouped vertically by region to assemble a barcode. Following, we analyzed 50 NIH 3T3 fibroblast movies, and obtained corresponding barcodes of each movie as a barcode collection (Supplementary Information 1). Using this collection, we calculated the overall occurrence rate of each region in all 50 samples, where occurrence percentage rates of Regions I, II, III, and IV were 24%, 42%, 24% and 11%, respectively. Consequently, we selected four individual barcodes in each separate region (Fig. 2, left), which possesses significantly higher percentile of CN correlation occurrence than the average occurrence rate of the corresponding region, and inspected their source movies.

View Article: PubMed Central - PubMed

ABSTRACT

Cell migration is an essential process in organism development and physiological maintenance. Although current methods permit accurate comparisons of the effects of molecular manipulations and drug applications on cell motility, effects of alterations in subcellular activities on motility cannot be fully elucidated from those methods. Here, we develop a strategy termed cell-nuclear (CN) correlation to parameterize represented dynamic subcellular activities and to quantify their contributions in mesenchymal-like migration. Based on the biophysical meaning of the CN correlation, we propose a cell migration potential index (CMPI) to measure cell motility. When the effectiveness of CMPI was evaluated with respect to one of the most popular cell migration analysis methods, Persistent Random Walk, we found that the cell motility estimates among six cell lines used in this study were highly consistent between these two approaches. Further evaluations indicated that CMPI can be determined using a shorter time period and smaller cell sample size, and it possesses excellent reliability and applicability, even in the presence of a wide range of noise, as might be generated from individual imaging acquisition systems. The novel approach outlined here introduces a robust strategy through an analysis of subcellular locomotion activities for single cell migration assessment.

No MeSH data available.


Related in: MedlinePlus