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Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus

View Article: PubMed Central - PubMed

ABSTRACT

Genic male sterility (GMS) has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus, however, its mechanisms of GMS remain largely unclear. Histological and Transmission electron microscopy (TEM) analyses of anthers showed that the male gamete development of TE5A was arrested at meiosis prophase I. EdU uptake of S-phase meiocytes revealed that the TE5A mutant could accomplish DNA replication, however, chromosomal and fluorescence in situ hybridization (FISH) analyses of TE5A showed that homologous chromosomes could not pair, synapse, condense and form bivalents. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants through RNA-Seq. A total of 3,841 differentially expressed genes (DEGs) were obtained, some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. Dynamic expression changes of selected candidate DEGs were then analyzed at different anther developmental stages. The present study not only demonstrated that the TE5A mutant had defects in meiotic prophase I via detailed cytological analysis, but also provided a global insight into GMS-associated DEGs and elucidated the mechanisms of GMS in TE5A through RNA-Seq.

No MeSH data available.


Dynamic expression changes of candidate DEGs at different developmental stages based on real-time PCR.A total of six DEGs, Fna021329, Fna087283, Fna048643, Fna063085, Fna016698, and Fna032795 were selected for further analysis at different anther development stages in the A1 and B1 lines. Relative levels of genes in real-time PCR are presented by 2−ΔΔCT.
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f9: Dynamic expression changes of candidate DEGs at different developmental stages based on real-time PCR.A total of six DEGs, Fna021329, Fna087283, Fna048643, Fna063085, Fna016698, and Fna032795 were selected for further analysis at different anther development stages in the A1 and B1 lines. Relative levels of genes in real-time PCR are presented by 2−ΔΔCT.

Mentions: RECQ HELICASES are involved in DNA recombination during later phases of DSB repair and are possibly related to branch migration at the Holliday junction52. In the present study, the differential expression of two DEGs encoding helicase, Fna021329, and Fna087283 were examined during above four anther developmental stages in A1 (sterile) and B1 (fertile) lines by using real-time PCR. Both Fna021329 and Fna087283 were not expressed during the four stages in line A1, whereas these were expressed at the PMC stage, and further upregulated at the meiosis, in the tetrad, and the microspore stage in the B1 line (Fig. 9).


Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus
Dynamic expression changes of candidate DEGs at different developmental stages based on real-time PCR.A total of six DEGs, Fna021329, Fna087283, Fna048643, Fna063085, Fna016698, and Fna032795 were selected for further analysis at different anther development stages in the A1 and B1 lines. Relative levels of genes in real-time PCR are presented by 2−ΔΔCT.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037387&req=5

f9: Dynamic expression changes of candidate DEGs at different developmental stages based on real-time PCR.A total of six DEGs, Fna021329, Fna087283, Fna048643, Fna063085, Fna016698, and Fna032795 were selected for further analysis at different anther development stages in the A1 and B1 lines. Relative levels of genes in real-time PCR are presented by 2−ΔΔCT.
Mentions: RECQ HELICASES are involved in DNA recombination during later phases of DSB repair and are possibly related to branch migration at the Holliday junction52. In the present study, the differential expression of two DEGs encoding helicase, Fna021329, and Fna087283 were examined during above four anther developmental stages in A1 (sterile) and B1 (fertile) lines by using real-time PCR. Both Fna021329 and Fna087283 were not expressed during the four stages in line A1, whereas these were expressed at the PMC stage, and further upregulated at the meiosis, in the tetrad, and the microspore stage in the B1 line (Fig. 9).

View Article: PubMed Central - PubMed

ABSTRACT

Genic male sterility (GMS) has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus, however, its mechanisms of GMS remain largely unclear. Histological and Transmission electron microscopy (TEM) analyses of anthers showed that the male gamete development of TE5A was arrested at meiosis prophase I. EdU uptake of S-phase meiocytes revealed that the TE5A mutant could accomplish DNA replication, however, chromosomal and fluorescence in situ hybridization (FISH) analyses of TE5A showed that homologous chromosomes could not pair, synapse, condense and form bivalents. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants through RNA-Seq. A total of 3,841 differentially expressed genes (DEGs) were obtained, some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. Dynamic expression changes of selected candidate DEGs were then analyzed at different anther developmental stages. The present study not only demonstrated that the TE5A mutant had defects in meiotic prophase I via detailed cytological analysis, but also provided a global insight into GMS-associated DEGs and elucidated the mechanisms of GMS in TE5A through RNA-Seq.

No MeSH data available.