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Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus

View Article: PubMed Central - PubMed

ABSTRACT

Genic male sterility (GMS) has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus, however, its mechanisms of GMS remain largely unclear. Histological and Transmission electron microscopy (TEM) analyses of anthers showed that the male gamete development of TE5A was arrested at meiosis prophase I. EdU uptake of S-phase meiocytes revealed that the TE5A mutant could accomplish DNA replication, however, chromosomal and fluorescence in situ hybridization (FISH) analyses of TE5A showed that homologous chromosomes could not pair, synapse, condense and form bivalents. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants through RNA-Seq. A total of 3,841 differentially expressed genes (DEGs) were obtained, some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. Dynamic expression changes of selected candidate DEGs were then analyzed at different anther developmental stages. The present study not only demonstrated that the TE5A mutant had defects in meiotic prophase I via detailed cytological analysis, but also provided a global insight into GMS-associated DEGs and elucidated the mechanisms of GMS in TE5A through RNA-Seq.

No MeSH data available.


Histogram showing Gene Ontology functional analysis of DEGs.The frequency of GO terms was analyzed by using GO Slim Assignment. The y-axis and x-axis indicated the names of clusters and the ratio of each cluster, respectively.
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f8: Histogram showing Gene Ontology functional analysis of DEGs.The frequency of GO terms was analyzed by using GO Slim Assignment. The y-axis and x-axis indicated the names of clusters and the ratio of each cluster, respectively.

Mentions: GO is a usual standardized gene analysis classification system which categorizes genes and gene products in terms of their biological process, cellular component, and molecular function. A total of 2,339 DEGs belonging to 37 different categories were recognized (Fig. 8). For the biological processes category, genes involved in metabolism were the predominant activity (31.5%), followed by those involved in cellular processes (24%), biological regulation (6.9%), and pigmentation (6.6%). Among those with a molecular function role, 37.3% were assigned to binding, 29.2% to catalytic, and 4.1% to transporter activities. For the cellular component category, 16.1% targeted cells and cell parts, 4.3% were directed toward organelles, and 2.2% to macromolecular complexes.


Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus
Histogram showing Gene Ontology functional analysis of DEGs.The frequency of GO terms was analyzed by using GO Slim Assignment. The y-axis and x-axis indicated the names of clusters and the ratio of each cluster, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037387&req=5

f8: Histogram showing Gene Ontology functional analysis of DEGs.The frequency of GO terms was analyzed by using GO Slim Assignment. The y-axis and x-axis indicated the names of clusters and the ratio of each cluster, respectively.
Mentions: GO is a usual standardized gene analysis classification system which categorizes genes and gene products in terms of their biological process, cellular component, and molecular function. A total of 2,339 DEGs belonging to 37 different categories were recognized (Fig. 8). For the biological processes category, genes involved in metabolism were the predominant activity (31.5%), followed by those involved in cellular processes (24%), biological regulation (6.9%), and pigmentation (6.6%). Among those with a molecular function role, 37.3% were assigned to binding, 29.2% to catalytic, and 4.1% to transporter activities. For the cellular component category, 16.1% targeted cells and cell parts, 4.3% were directed toward organelles, and 2.2% to macromolecular complexes.

View Article: PubMed Central - PubMed

ABSTRACT

Genic male sterility (GMS) has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus, however, its mechanisms of GMS remain largely unclear. Histological and Transmission electron microscopy (TEM) analyses of anthers showed that the male gamete development of TE5A was arrested at meiosis prophase I. EdU uptake of S-phase meiocytes revealed that the TE5A mutant could accomplish DNA replication, however, chromosomal and fluorescence in situ hybridization (FISH) analyses of TE5A showed that homologous chromosomes could not pair, synapse, condense and form bivalents. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants through RNA-Seq. A total of 3,841 differentially expressed genes (DEGs) were obtained, some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. Dynamic expression changes of selected candidate DEGs were then analyzed at different anther developmental stages. The present study not only demonstrated that the TE5A mutant had defects in meiotic prophase I via detailed cytological analysis, but also provided a global insight into GMS-associated DEGs and elucidated the mechanisms of GMS in TE5A through RNA-Seq.

No MeSH data available.