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Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus

View Article: PubMed Central - PubMed

ABSTRACT

Genic male sterility (GMS) has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus, however, its mechanisms of GMS remain largely unclear. Histological and Transmission electron microscopy (TEM) analyses of anthers showed that the male gamete development of TE5A was arrested at meiosis prophase I. EdU uptake of S-phase meiocytes revealed that the TE5A mutant could accomplish DNA replication, however, chromosomal and fluorescence in situ hybridization (FISH) analyses of TE5A showed that homologous chromosomes could not pair, synapse, condense and form bivalents. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants through RNA-Seq. A total of 3,841 differentially expressed genes (DEGs) were obtained, some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. Dynamic expression changes of selected candidate DEGs were then analyzed at different anther developmental stages. The present study not only demonstrated that the TE5A mutant had defects in meiotic prophase I via detailed cytological analysis, but also provided a global insight into GMS-associated DEGs and elucidated the mechanisms of GMS in TE5A through RNA-Seq.

No MeSH data available.


Changes in gene expression in A1 and B1 libraries.The number of upregulated and downregulated genes specific to A1 and specific to B1 are summarized.
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f7: Changes in gene expression in A1 and B1 libraries.The number of upregulated and downregulated genes specific to A1 and specific to B1 are summarized.

Mentions: There were 3,841 genes in B. napus that were detected with significant differential expression levels between A1 and B1 (Supplementary file 1). These included both 2,939 upregulated and 902 downregulated genes in B1. Of the 2,939 upregulated DEGs in B1, 511 genes were uniquely expressed in B1, which were postulated to participate in the normal development of male gametes, and only 16 of the 902 downregulated genes in B1 were specially expressed in A1, which were assumed to be involved in the abnormal development of male gametes (Fig. 7).


Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus
Changes in gene expression in A1 and B1 libraries.The number of upregulated and downregulated genes specific to A1 and specific to B1 are summarized.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037387&req=5

f7: Changes in gene expression in A1 and B1 libraries.The number of upregulated and downregulated genes specific to A1 and specific to B1 are summarized.
Mentions: There were 3,841 genes in B. napus that were detected with significant differential expression levels between A1 and B1 (Supplementary file 1). These included both 2,939 upregulated and 902 downregulated genes in B1. Of the 2,939 upregulated DEGs in B1, 511 genes were uniquely expressed in B1, which were postulated to participate in the normal development of male gametes, and only 16 of the 902 downregulated genes in B1 were specially expressed in A1, which were assumed to be involved in the abnormal development of male gametes (Fig. 7).

View Article: PubMed Central - PubMed

ABSTRACT

Genic male sterility (GMS) has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus, however, its mechanisms of GMS remain largely unclear. Histological and Transmission electron microscopy (TEM) analyses of anthers showed that the male gamete development of TE5A was arrested at meiosis prophase I. EdU uptake of S-phase meiocytes revealed that the TE5A mutant could accomplish DNA replication, however, chromosomal and fluorescence in situ hybridization (FISH) analyses of TE5A showed that homologous chromosomes could not pair, synapse, condense and form bivalents. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants through RNA-Seq. A total of 3,841 differentially expressed genes (DEGs) were obtained, some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. Dynamic expression changes of selected candidate DEGs were then analyzed at different anther developmental stages. The present study not only demonstrated that the TE5A mutant had defects in meiotic prophase I via detailed cytological analysis, but also provided a global insight into GMS-associated DEGs and elucidated the mechanisms of GMS in TE5A through RNA-Seq.

No MeSH data available.