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Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus

View Article: PubMed Central - PubMed

ABSTRACT

Genic male sterility (GMS) has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus, however, its mechanisms of GMS remain largely unclear. Histological and Transmission electron microscopy (TEM) analyses of anthers showed that the male gamete development of TE5A was arrested at meiosis prophase I. EdU uptake of S-phase meiocytes revealed that the TE5A mutant could accomplish DNA replication, however, chromosomal and fluorescence in situ hybridization (FISH) analyses of TE5A showed that homologous chromosomes could not pair, synapse, condense and form bivalents. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants through RNA-Seq. A total of 3,841 differentially expressed genes (DEGs) were obtained, some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. Dynamic expression changes of selected candidate DEGs were then analyzed at different anther developmental stages. The present study not only demonstrated that the TE5A mutant had defects in meiotic prophase I via detailed cytological analysis, but also provided a global insight into GMS-associated DEGs and elucidated the mechanisms of GMS in TE5A through RNA-Seq.

No MeSH data available.


Model of GMS in TE5A.The network of GMS in TE5A based on the analysis of cytological observation and RNA-seq. Red boxes indicate no difference between the wild-type and the TE5A mutant.
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f10: Model of GMS in TE5A.The network of GMS in TE5A based on the analysis of cytological observation and RNA-seq. Red boxes indicate no difference between the wild-type and the TE5A mutant.

Mentions: Cytological observation and the differential gene expression data generated from RNA-Seq indicate that TE5A mutants can be utilized as a model for GMS (Fig. 10). Successful male development are related to various important development events, including cell differentiation, meristem specification, communication among cells, mitosis and meiosis5160. Paraffin-cross sections of anthers showed that male gamete development was arrested at the first meiotic division stage, and TEM analysis of cross sections of anthers indicated that PMCs were arrested at prophase I, which eventually resulted in the degeneration of meiotic PMCs in TE5A. Meiotic prophase I in the wild-type is characterized by pairing, chromosome cohesion, and recombination51. Chromosome spreads and FISH showed that homologous chromosomes could not pair, synapse, condense, and form bivalents in the TE5A mutant. RNA-Seq further uncovered DEGs associated with the defects observed during TE5A mutant gametogenesis, including DEGs that were involved in homologous chromosome behavior and the cycle control during meiosis.


Aberrant Meiotic Prophase I Leads to Genic Male Sterility in the Novel TE5A Mutant of Brassica napus
Model of GMS in TE5A.The network of GMS in TE5A based on the analysis of cytological observation and RNA-seq. Red boxes indicate no difference between the wild-type and the TE5A mutant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037387&req=5

f10: Model of GMS in TE5A.The network of GMS in TE5A based on the analysis of cytological observation and RNA-seq. Red boxes indicate no difference between the wild-type and the TE5A mutant.
Mentions: Cytological observation and the differential gene expression data generated from RNA-Seq indicate that TE5A mutants can be utilized as a model for GMS (Fig. 10). Successful male development are related to various important development events, including cell differentiation, meristem specification, communication among cells, mitosis and meiosis5160. Paraffin-cross sections of anthers showed that male gamete development was arrested at the first meiotic division stage, and TEM analysis of cross sections of anthers indicated that PMCs were arrested at prophase I, which eventually resulted in the degeneration of meiotic PMCs in TE5A. Meiotic prophase I in the wild-type is characterized by pairing, chromosome cohesion, and recombination51. Chromosome spreads and FISH showed that homologous chromosomes could not pair, synapse, condense, and form bivalents in the TE5A mutant. RNA-Seq further uncovered DEGs associated with the defects observed during TE5A mutant gametogenesis, including DEGs that were involved in homologous chromosome behavior and the cycle control during meiosis.

View Article: PubMed Central - PubMed

ABSTRACT

Genic male sterility (GMS) has already been extensively utilized for hybrid rapeseed production. TE5A is a novel thermo-sensitive dominant GMS line in Brassica napus, however, its mechanisms of GMS remain largely unclear. Histological and Transmission electron microscopy (TEM) analyses of anthers showed that the male gamete development of TE5A was arrested at meiosis prophase I. EdU uptake of S-phase meiocytes revealed that the TE5A mutant could accomplish DNA replication, however, chromosomal and fluorescence in situ hybridization (FISH) analyses of TE5A showed that homologous chromosomes could not pair, synapse, condense and form bivalents. We then analyzed the transcriptome differences between young floral buds of sterile plants and its near-isogenic fertile plants through RNA-Seq. A total of 3,841 differentially expressed genes (DEGs) were obtained, some of which were associated with homologous chromosome behavior and cell cycle control during meiosis. Dynamic expression changes of selected candidate DEGs were then analyzed at different anther developmental stages. The present study not only demonstrated that the TE5A mutant had defects in meiotic prophase I via detailed cytological analysis, but also provided a global insight into GMS-associated DEGs and elucidated the mechanisms of GMS in TE5A through RNA-Seq.

No MeSH data available.