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Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light

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ABSTRACT

Photobiomodulation (PBM) with blue light induces a biphasic dose response curve in proliferation of immortalized human keratinocytes (HaCaT), with a maximum anti-proliferative effect reached with 30min (41.4 J/cm2). The aim of this study was to test the photobiomodulatory effect of 41.4 J/cm2 blue light irradiation on ROS production, apoptosis and gene expression at different time points after irradiation of HaCaT cells in vitro and assess its safety. ROS concentration was increased 30 min after irradiation. However, already 1 h after irradiation, cells were able to reduce ROS and balance the concentration to a normal level. The sudden increase in ROS did not damage the cells, which was demonstrated with FACS analysis where HaCaT cells did not show any sign of apoptosis after blue light irradiation. Furthermore, a time course could be seen in gene expression analysis after blue light, with an early response of stimulated genes already 1 h after blue light irradiation, leading to the discovery of the aryl hydrocarbon receptor as possible target for blue light irradiation.

No MeSH data available.


Gene expression analysis-volcano plot 24 h after blue light irradiation.
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f3: Gene expression analysis-volcano plot 24 h after blue light irradiation.

Mentions: Genes that stand out are cytochrome P450 family 1 subfamily A member 1 (CYP1A1) and CYP1B1, which are both highly upregulated for all three harvesting time points, with significant p-values for 3 h and 24 h (Fig. 3 and Supplementary data 1-2).


Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light
Gene expression analysis-volcano plot 24 h after blue light irradiation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037386&req=5

f3: Gene expression analysis-volcano plot 24 h after blue light irradiation.
Mentions: Genes that stand out are cytochrome P450 family 1 subfamily A member 1 (CYP1A1) and CYP1B1, which are both highly upregulated for all three harvesting time points, with significant p-values for 3 h and 24 h (Fig. 3 and Supplementary data 1-2).

View Article: PubMed Central - PubMed

ABSTRACT

Photobiomodulation (PBM) with blue light induces a biphasic dose response curve in proliferation of immortalized human keratinocytes (HaCaT), with a maximum anti-proliferative effect reached with 30min (41.4 J/cm2). The aim of this study was to test the photobiomodulatory effect of 41.4 J/cm2 blue light irradiation on ROS production, apoptosis and gene expression at different time points after irradiation of HaCaT cells in vitro and assess its safety. ROS concentration was increased 30 min after irradiation. However, already 1 h after irradiation, cells were able to reduce ROS and balance the concentration to a normal level. The sudden increase in ROS did not damage the cells, which was demonstrated with FACS analysis where HaCaT cells did not show any sign of apoptosis after blue light irradiation. Furthermore, a time course could be seen in gene expression analysis after blue light, with an early response of stimulated genes already 1 h after blue light irradiation, leading to the discovery of the aryl hydrocarbon receptor as possible target for blue light irradiation.

No MeSH data available.