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Biocompatibility and biodistribution of functionalized carbon nano-onions (f-CNOs) in a vertebrate model

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ABSTRACT

Functionalized carbon nano-onions (f-CNOs) are of great interest as platforms for imaging, diagnostic and therapeutic applications due to their high cellular uptake and low cytotoxicity. To date, the toxicological effects of f-CNOs on vertebrates have not been reported. In this study, the possible biological impact of f-CNOs on zebrafish during development is investigated, evaluating different toxicity end-points such as the survival rate, hatching rate, and heart beat rate. Furthermore, a bio-distribution study of boron dipyrromethene (BODIPY) functionalized CNOs in zebrafish larvae is performed by utilizing inverted selective plane illumination microscopy (iSPIM), due to its intrinsic capability of allowing for fast 3D imaging. Our in vivo findings indicate that f-CNOs exhibit no toxicity, good biocompatibility (in the concentration range of 5–100 μg mL−1) and a homogenous biodistribution in zebrafish larvae.

No MeSH data available.


Heart beat rate of zebrafish larvae at 72 hpf exposed to different concentration of benz-CNOs (a) and BODIPY-CNOs (b) and frequency of voluntary movements of the larvae at 72 hpf exposed to different concentration of benz-CNOs (c) and BODIPY-CNOs (d). Data are calculated as means ± S.D., from three independent experiments, n = 80 (*p ≤ 0.01 compared to the control).
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f3: Heart beat rate of zebrafish larvae at 72 hpf exposed to different concentration of benz-CNOs (a) and BODIPY-CNOs (b) and frequency of voluntary movements of the larvae at 72 hpf exposed to different concentration of benz-CNOs (c) and BODIPY-CNOs (d). Data are calculated as means ± S.D., from three independent experiments, n = 80 (*p ≤ 0.01 compared to the control).

Mentions: Furthermore, the heart beat rate and frequency of movements in zebrafish larvae at 72 hpf is monitored for abnormalities induced by the CNOs exposure. The number of heart beats for the larvae treated with benz-CNOs (144–149/min) and BODIPY-CNOs (146–150/min) is comparable to control group (150 beats/min) (Fig. 3a,b). The frequency of movements, as response to touching, is of 4–4.6 /min and of 4.3–4.6 /min for the ones treated with the benz-CNOs and BODIPY-CNOs, respectively, without change compared to that of the control (4.6 /min) (Fig. 3c,d).The results obtained show no significant adverse effects of CNOs on both the parameters.


Biocompatibility and biodistribution of functionalized carbon nano-onions (f-CNOs) in a vertebrate model
Heart beat rate of zebrafish larvae at 72 hpf exposed to different concentration of benz-CNOs (a) and BODIPY-CNOs (b) and frequency of voluntary movements of the larvae at 72 hpf exposed to different concentration of benz-CNOs (c) and BODIPY-CNOs (d). Data are calculated as means ± S.D., from three independent experiments, n = 80 (*p ≤ 0.01 compared to the control).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5037369&req=5

f3: Heart beat rate of zebrafish larvae at 72 hpf exposed to different concentration of benz-CNOs (a) and BODIPY-CNOs (b) and frequency of voluntary movements of the larvae at 72 hpf exposed to different concentration of benz-CNOs (c) and BODIPY-CNOs (d). Data are calculated as means ± S.D., from three independent experiments, n = 80 (*p ≤ 0.01 compared to the control).
Mentions: Furthermore, the heart beat rate and frequency of movements in zebrafish larvae at 72 hpf is monitored for abnormalities induced by the CNOs exposure. The number of heart beats for the larvae treated with benz-CNOs (144–149/min) and BODIPY-CNOs (146–150/min) is comparable to control group (150 beats/min) (Fig. 3a,b). The frequency of movements, as response to touching, is of 4–4.6 /min and of 4.3–4.6 /min for the ones treated with the benz-CNOs and BODIPY-CNOs, respectively, without change compared to that of the control (4.6 /min) (Fig. 3c,d).The results obtained show no significant adverse effects of CNOs on both the parameters.

View Article: PubMed Central - PubMed

ABSTRACT

Functionalized carbon nano-onions (f-CNOs) are of great interest as platforms for imaging, diagnostic and therapeutic applications due to their high cellular uptake and low cytotoxicity. To date, the toxicological effects of f-CNOs on vertebrates have not been reported. In this study, the possible biological impact of f-CNOs on zebrafish during development is investigated, evaluating different toxicity end-points such as the survival rate, hatching rate, and heart beat rate. Furthermore, a bio-distribution study of boron dipyrromethene (BODIPY) functionalized CNOs in zebrafish larvae is performed by utilizing inverted selective plane illumination microscopy (iSPIM), due to its intrinsic capability of allowing for fast 3D imaging. Our in vivo findings indicate that f-CNOs exhibit no toxicity, good biocompatibility (in the concentration range of 5–100 μg mL−1) and a homogenous biodistribution in zebrafish larvae.

No MeSH data available.