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The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons

View Article: PubMed Central - HTML - PubMed

ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.


Spontaneous activity in OSNs from I7-GFP WT and KO mice for TMEM16B. (A and B) Loose-patch recordings (A; 60-s recordings) and raster plots (B) of the spontaneous firing activity from three WT OSNs (top) and three KO mice (bottom). (C and E) Cumulative plot of spontaneous mean (C) and instantaneous (E) firing frequency, bin of 0.1 Hz, in control (black lines) and TMEM16B KO mice (blue lines). Mann-Whitney U test, one-tail: **, P < 0.01 in C; and ***, P < 0.001 in E. (D and F) Box and scatter plots of spontaneous mean (D) and instantaneous (F) firing activity in OSNs in WT and KO mice. In the box plots, the inner squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. (G) ISI distribution histogram (bin = 5 ms) normalized to the number of recorded neurons in control (black line, n = 34 from 19 mice) and KO mice (blue line, n = 22 from 9 mice).
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fig8: Spontaneous activity in OSNs from I7-GFP WT and KO mice for TMEM16B. (A and B) Loose-patch recordings (A; 60-s recordings) and raster plots (B) of the spontaneous firing activity from three WT OSNs (top) and three KO mice (bottom). (C and E) Cumulative plot of spontaneous mean (C) and instantaneous (E) firing frequency, bin of 0.1 Hz, in control (black lines) and TMEM16B KO mice (blue lines). Mann-Whitney U test, one-tail: **, P < 0.01 in C; and ***, P < 0.001 in E. (D and F) Box and scatter plots of spontaneous mean (D) and instantaneous (F) firing activity in OSNs in WT and KO mice. In the box plots, the inner squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. (G) ISI distribution histogram (bin = 5 ms) normalized to the number of recorded neurons in control (black line, n = 34 from 19 mice) and KO mice (blue line, n = 22 from 9 mice).

Mentions: Data in Figs. 5, 6, 7, and 8 are presented as mean value and boxplots in which the inner square represents the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. Spontaneous and evoked firing frequency data were not in all cases normally distributed (Kolmogorov-Smirnov test or Shapiro-Wilkinson test), and statistical significance was determined using Mann-Whitney U test. P-values <0.05 were considered statistically significant.


The Ca 2+ -activated Cl − channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons
Spontaneous activity in OSNs from I7-GFP WT and KO mice for TMEM16B. (A and B) Loose-patch recordings (A; 60-s recordings) and raster plots (B) of the spontaneous firing activity from three WT OSNs (top) and three KO mice (bottom). (C and E) Cumulative plot of spontaneous mean (C) and instantaneous (E) firing frequency, bin of 0.1 Hz, in control (black lines) and TMEM16B KO mice (blue lines). Mann-Whitney U test, one-tail: **, P < 0.01 in C; and ***, P < 0.001 in E. (D and F) Box and scatter plots of spontaneous mean (D) and instantaneous (F) firing activity in OSNs in WT and KO mice. In the box plots, the inner squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. (G) ISI distribution histogram (bin = 5 ms) normalized to the number of recorded neurons in control (black line, n = 34 from 19 mice) and KO mice (blue line, n = 22 from 9 mice).
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fig8: Spontaneous activity in OSNs from I7-GFP WT and KO mice for TMEM16B. (A and B) Loose-patch recordings (A; 60-s recordings) and raster plots (B) of the spontaneous firing activity from three WT OSNs (top) and three KO mice (bottom). (C and E) Cumulative plot of spontaneous mean (C) and instantaneous (E) firing frequency, bin of 0.1 Hz, in control (black lines) and TMEM16B KO mice (blue lines). Mann-Whitney U test, one-tail: **, P < 0.01 in C; and ***, P < 0.001 in E. (D and F) Box and scatter plots of spontaneous mean (D) and instantaneous (F) firing activity in OSNs in WT and KO mice. In the box plots, the inner squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. (G) ISI distribution histogram (bin = 5 ms) normalized to the number of recorded neurons in control (black line, n = 34 from 19 mice) and KO mice (blue line, n = 22 from 9 mice).
Mentions: Data in Figs. 5, 6, 7, and 8 are presented as mean value and boxplots in which the inner square represents the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. Spontaneous and evoked firing frequency data were not in all cases normally distributed (Kolmogorov-Smirnov test or Shapiro-Wilkinson test), and statistical significance was determined using Mann-Whitney U test. P-values <0.05 were considered statistically significant.

View Article: PubMed Central - HTML - PubMed

ABSTRACT

TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.

No MeSH data available.